LOCUS AI271714 568 bp mRNA linear EST 28-JAN-1999 DEFINITION qj87g04.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1866486 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI271714 VERSION AI271714.1 GI:3890881 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 568) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1163 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 407. FEATURES Location/Qualifiers source 1..568 /db_xref="taxon:9606" /clone="IMAGE:1866486" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 112 a 174 c 189 g 91 t 2 others ORIGIN 1 ttttgcttgt atcacctgct ctttattgat tgccgcgggc tgcgctctca tgggcgaccc 61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc 121 cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc ccaggaggtg 181 tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct ggccggaggt 241 caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg ggagccaggc 301 tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt cgggtctggg 361 acaccctgag accctaagct ctcctggctg ctctctcaaa ccctcattcc atgtgaaaag 421 gtggaaagga aaacagggaa gcaaaggaag acagacaccc cgcccctcag gtgctccccc 481 catgatgggc cagggctgca ggggagccag ccgaggcaat gcctgcttan gacgagggag 541 gccgagtggg gctcntctcc cgcgacac // LOCUS AA452024 549 bp mRNA linear EST 05-JUN-1997 DEFINITION zw57c10.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:774162 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA452024 VERSION AA452024.1 GI:2165693 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 549) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B. , Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie ,T., Waterston,R. and Wilson,R. TITLE WashU-Merck EST Project 1997 JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -41m13 fwd. ET from Amersham High quality sequence stop: 476. FEATURES Location/Qualifiers source 1..549 /db_xref="taxon:9606" /clone="IMAGE:774162" /lab_host="DH10B" /dev_stage="8-9 weeks" /organism="Homo sapiens" /clone_lib="Soares_total_fetus_Nb2HF8_9w" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 111 a 156 c 189 g 93 t ORIGIN 1 tttttgcttg tatcacctgc tgtttattga ttgccgcggg tgcgctctca tgggcgaccc 61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc 121 cgcgagagcc taggtgcaca gaagccccgg ccccacggct tcacgcgccc caggaggtgt 181 gggcagggcg ctcgcggagg gaggctgcag gtgtgtcagg aggcacctgg ccggagtgca 241 ccccctcctt ctggaccctc agcagagttg aggcgcctgc accccagggg agccaggctg 301 ctatctaaag gagtgtgagg tgcggtcact tccgccactg gccggtgtcg gggtctggga 361 caccctgaga ccctaagctc tcctggctgc tctctcaaac cctcattcca tgtgagaagg 421 tggaaaggaa aaaagggaag caaaggaaga cagacacccc gccctcaggg tgctcccccc 481 atgatgggcc agggctgcag gggagccagc cgaggcaatt gctgcttagg acgagggaag 541 ccaggtggg // LOCUS AA743802 516 bp mRNA linear EST 23-JAN-1998 DEFINITION ob01b06.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1322387 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA743802 VERSION AA743802.1 GI:2784618 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 516) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov unknown library type Insert Length: 1221 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 481. FEATURES Location/Qualifiers source 1..516 /db_xref="taxon:9606" /clone="IMAGE:1322387" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 103 a 154 c 175 g 84 t ORIGIN 1 ttttgcttgt atcacctgct gtttattgat tgccgcgggt gcgctctcat gggcgaccca 61 tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag agaagaggcc 121 gcgagaccca ggtgcacaga agccccggcc ccacggcgtc cacgcgcccc aggaggtgtg 181 ggcagggccg cgcgcggagg gaggctgcag gtgtgtcagg aggcacctgt gccggagtca 241 ccccctcctt ctggaccctc agcagagttg aggcgcctgc accccagggg agccaggctg 301 ctatctaaag gagtgtgagg tgcggtcact tccgccactg gccggtgtcg gggtctggga 361 caccctgaga ccctaagctc tcctggctgc tctctcaaac cctcattcca tgtgagaagg 421 tggaaaggaa aaaagggaag caaaggaaga cagacacccc gccctcaggg tgctcccccc 481 atgatgggcc agggctgcag gggagccagc cgaggc // LOCUS AI262258 432 bp mRNA linear EST 28-JAN-1999 DEFINITION qk09h12.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1868519 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI262258 VERSION AI262258.1 GI:3870461 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 432) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 528 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..432 /db_xref="taxon:9606" /clone="IMAGE:1868519" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 84 a 129 c 146 g 72 t 1 others ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgncggg gtctgggaca 361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg 421 gaaaggaaaa aa // LOCUS AA429819 455 bp mRNA linear EST 16-OCT-1997 DEFINITION zw57c10.r1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:774162 5' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA429819 VERSION AA429819.1 GI:2112992 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 455) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B. , Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie ,T., Waterston,R. and Wilson,R. TITLE WashU-Merck EST Project 1997 JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 416. FEATURES Location/Qualifiers source 1..455 /db_xref="taxon:9606" /clone="IMAGE:774162" /lab_host="DH10B" /dev_stage="8-9 weeks" /organism="Homo sapiens" /clone_lib="Soares_total_fetus_Nb2HF8_9w" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 76 a 168 c 117 g 94 t ORIGIN 1 ggcctttttg agtatgacct gctgcccttc ctgtcgtcca ccatcagccc ctactaccgc 61 cagtccctct tccgcaccgt gctggactcc ggcatctctg aggttcgatc cgaccgggac 121 aagttcgtca tcttcctcga tgtgaagcac ttctccccgg agacctcacc gtgaaggtgc 181 aggacgactt tgtggagatc cacggaaagc acaacgagcg ccaggacgac cacggctaca 241 tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag tcggccctct 301 cttgctccct gtctgccgat gcatgctgac cttctgtggc cccaagatcc agactggcct 361 ggatgccacc cacgccgagc gagccatccc cgtgtcgcgg gaggagaagc ccactcggct 421 ccctcgtcct gagcaggcat tgctcggctg gctcc // LOCUS AA886899 557 bp mRNA linear EST 09-JUN-1998 DEFINITION oj26h11.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1493349 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA886899 VERSION AA886899.1 GI:3002007 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 557) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Trace considered overall poor quality Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Trace considered overall poor quality Insert Length: 1118 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..557 /db_xref="taxon:9606" /clone="IMAGE:1493349" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 115 a 165 c 187 g 89 t 1 others ORIGIN 1 ttgaatcacc tgctctttat tgattgccgc gggtgcgctc tcatgggcga cccatgtccc 61 caagagcggc actacataga ggggttggag gaagacggtc tgagagaaga ggccgcgaga 121 cccaaggtgc acagaagccc cggccccacg cgtccacgcg ccccaggagg tgtgggcagg 181 gccgccgcgg agggaggctg caggtgtgtc aggaggcacc tggcggaagt tcaccccctc 241 cttctggacc ctcagcagag ttgaggcgcc tgaaccccag gggagccagg ctgctatcta 301 aaggagtgtg aggtgcggtc acttccgcca ctggccggtg tcggggtctg ggacaccctg 361 agaccctaag ctctcctggc tgctctctca aaccctcatt ccatgtgaga aggtggaaag 421 gaaaacaggg aagcaaagga agacagacac cccgcccttc aggttgctcc cccatgatgg 481 gccagggctg caggggagcc accggaggaa tgctgcttan gacgaggagc cgagtggggc 541 tctctcccgc acacggg // LOCUS AI277590 517 bp mRNA linear EST 29-JAN-1999 DEFINITION qm55e12.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE:1892686 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI277590 VERSION AI277590.1 GI:3899858 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 517) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 803 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 352. FEATURES Location/Qualifiers source 1..517 /db_xref="taxon:9606" /clone="IMAGE:1892686" /lab_host="DH10B (ampicillin resistant)" /dev_stage="two placentae: one from 8 weeks and another from 9 weeks post conception" /organism="Homo sapiens" /clone_lib="Soares_placenta_8to9weeks_2NbHP8to9W" /note="Organ: placenta; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 108 a 134 c 179 g 95 t 1 others ORIGIN 1 ttttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga aggtggaaag 121 gaaaaaaggg aagcaaagga agacagacac cccgccctca gggtgctccc cccatgatgg 181 gccagggctg caggggagcc agccgaggca atgcctgctt aggacgaggg agccgaggtg 241 ggcttctcct cccgcgacac ggggatggct cgctcggcgt gggtggcatc caggccagtc 301 tggatcttgg ggccacagaa ggtcagcatg ccatcggcag acagggagca agagagggcc 361 gactggtcca cgttggacgg cangcggtag cggcggtgga actcacggga aatgtagccg 421 tgtcgtcctg ccgctcgttg tgctttccgt ggatctcaca agtcgttctg cactttaccg 481 gtgagtctcc ggggagaatg cttacatcca ggaaatg // LOCUS AI240847 419 bp mRNA linear EST 28-JAN-1999 DEFINITION qj99e03.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1867612 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI240847 VERSION AI240847.1 GI:3836244 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 419) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 479 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 366. FEATURES Location/Qualifiers source 1..419 /db_xref="taxon:9606" /clone="IMAGE:1867612" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 76 a 126 c 126 g 91 t ORIGIN 1 tttttttttt tttttttttt tttgcttgta tcacctgctg tttattgatt gccgcgggct 61 gcgctctcat gggcgaccca tgtccccaag agcggcacta cataaagggg ttggaggaaa 121 acggtctgaa aaaaaaggcc gcgagagccc aggtgcacaa aagccccggc cccacgggcc 181 tcacgccccc caggaggtgt gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag 241 gaggcacctg gccggaggtc accccctcct tctggaccct cagcaaagtt gaggcgcctg 301 caccccaggg gagccaggct gctatctaaa ggagtgtgag gtgcggtcac ttccgccact 361 ggccggtgtc gggtctgggc accctgaacc ctaactctcc tggctgtctc taaaccctc 421 // LOCUS AA772980 326 bp mRNA linear EST 29-JAN-1998 DEFINITION ab67a07.r1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE:851988 5' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA772980 VERSION AA772980.1 GI:2825822 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 326) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin ,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -28m13 rev1 ET from Amersham High quality sequence stop: 56. FEATURES Location/Qualifiers source 1..326 /db_xref="taxon:9606" /clone="IMAGE:851988" /lab_host="SOLR (kanamycin resistant)" /sex="mixed" /organism="Homo sapiens" /clone_lib="Stratagene fetal retina 937202" /note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Cloned unidirectionally. Primer: Oligo dT. Pooled retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'" BASE COUNT 63 a 99 c 118 g 46 t ORIGIN 1 gaccatgtgc ccaagagcgg cactacatag aggggttgga ggaagacggt ctgagagaag 61 aggccgcgag agccgaggtg cacagaagcc ccggccccac gcgtgcacgc gccccaggag 121 gtgtgggcag ggccgccgcg aagggaggct gcaggtgtgt caggaggcac ctggccggag 181 tcaccccctc cttctggacc ctcagcagag ttgaggcgcc tgcaccccag gggagccagg 241 ctgctatcta aaggagtgtg aggtgcggtc acttccgcca ctggccggtg ttcgggtctg 301 ggacaccctg agaccctaag ctctcc // LOCUS AA931460 320 bp mRNA linear EST 23-JUN-1998 DEFINITION om89d02.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1554339 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains PTR5.b2 TAR1 repetitive element ;, mRNA sequence. ACCESSION AA931460 VERSION AA931460.1 GI:3085846 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 320) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 2167 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham. FEATURES Location/Qualifiers source 1..320 /db_xref="taxon:9606" /clone="IMAGE:1554339" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 53 a 88 c 103 g 76 t ORIGIN 1 tttttttttt tttttttttt tttttttttt ttgcttgtat cacctgctgt ttattgattg 61 ccgcgggtgg cgctctcatg ggcgacccat gtccccaaga gcggcactac atagaggggt 121 tggaggaaga cggtctgaga gaagaggccg cgagacccat ggtgcacaga agccccggcc 181 ccacggctgc acgcgcccca ggaggtgtgg gcagggcgca cgccgaggga ggctgcaggt 241 gtgtcaggag gcacctggcc ggagtcaccc cctccttctg gaccctcagc agagttgagg 301 cgcctgcacc ccaggggagc // LOCUS AA490149 142 bp mRNA linear EST 05-MAR-1998 DEFINITION ab05g09.s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE:839968 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AA490149 VERSION AA490149.1 GI:2221024 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 142) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin ,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 319 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham. FEATURES Location/Qualifiers source 1..142 /db_xref="taxon:9606" /clone="IMAGE:839968" /lab_host="SOLR (kanamycin resistant)" /sex="mixed" /organism="Homo sapiens" /clone_lib="Stratagene fetal retina 937202" /note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Cloned unidirectionally. Primer: Oligo dT. Pooled retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'" BASE COUNT 31 a 36 c 48 g 27 t ORIGIN 1 tgcttgtatc acctgctgtt tattgattgc cgcgggctgc gctctcatgg gcgacccatg 61 tccccaagag cggcactaca tagaggggtt ggaggaagac ggtctgagag aagaggccgc 121 gagagcccag gtgcacagaa gc // LOCUS AI961742 499 bp mRNA linear EST 09-MAR-2000 DEFINITION wt66f11.x1 Soares_thymus_NHFTh Homo sapiens cDNA clone IMAGE:2512461 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI961742 VERSION AI961742.1 GI:5754455 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 499) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 893 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 410. FEATURES Location/Qualifiers source 1..499 /db_xref="taxon:9606" /clone="IMAGE:2512461" /lab_host="DH10B (phage-resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="Soares_thymus_NHFTh" /note="Organ: thymus, pooled; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAACGTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 99 a 151 c 167 g 80 t 2 others ORIGIN 1 tgcttgtatc acctgctgtt tattgattgc cgcgggctgc gctctcatgg gcgacccatg 61 tccccaagag cggcactaca tagaggggtt ggaggaagac ggtctgagag aagaggccgc 121 gagagcccag gtgcacagaa gccccggccc cacgggcctc acgcgcccca ggaggtgtgg 181 gcagggcccg ccgcggaggg aggctgcagg tgtgtcagga ggcacctggc cggaggtcac 241 cccctccttc tggaccctca gcagagttga ggcgcctgca ccccagggga gccaggctgc 301 tatctaaagg agtgtgaggt gcggtcactt ccgccactgg ccggtgtcgg ngtctgggac 361 accctgagac cctaagctct cctggctgct ctctcaaacc ctcattccat gtgagaaggt 421 ggaaaggaaa aaagggaagc anaggaagac agacaccccg ccctcagggt gctcccccca 481 tgatgggcca gggctgcag // LOCUS AI640776 496 bp mRNA linear EST 16-DEC-1999 DEFINITION wa15d09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2298161 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI640776 VERSION AI640776.1 GI:4703885 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 496) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 733 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 434. FEATURES Location/Qualifiers source 1..496 /db_xref="taxon:9606" /clone="IMAGE:2298161" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 105 a 150 c 161 g 80 t ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaaaagc ggcactacat aaaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacataag ccccggcccc acgggcctca cgcgccccat gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaagag gcacctgtcc ggaggtcacc 241 cactccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggacacttc cgccactggc cggagtcggg gtctgggaca 361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg 421 gaaaggaaaa aagggaagca aaggaagaca gacaccccgc cctcagggtg ctccccccat 481 gatgggccag ggctgc // LOCUS AI671484 393 bp mRNA linear EST 17-DEC-1999 DEFINITION wc30g10.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2316738 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI671484 VERSION AI671484.1 GI:4851215 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 393) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 489 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..393 /db_xref="taxon:9606" /clone="IMAGE:2316738" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 67 a 122 c 137 g 67 t ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtcggg gtctgggaca 361 ccctgagacc ctaagctctc ctggctgctc tct // LOCUS AI472821 447 bp mRNA linear EST 13-APR-1999 DEFINITION tj70f11.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:2146893 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI472821 VERSION AI472821.1 GI:4325866 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 447) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1029 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 295. FEATURES Location/Qualifiers source 1..447 /db_xref="taxon:9606" /clone="IMAGE:2146893" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="Soares_NSF_F8_9W_OT_PA_P_S1" /note="Organ: pooled; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from five normalized libraries were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 5 libraries. The pools consisted of the following libraries and cloneIDs: Soares NbHSF pool 1: 309384-310919, 323208-325895 Soares Nb2HP pool 1: 145032-147335, 147720-148103, 148872-149255, 15002 - 150407, 151176-152327 Soares Nb2HF8-9W pool 1: 758280-760583, 772104-774407 Soares NbHPA pool 1: 304776-306311, 320136-322823, 326280-326663 Soares NbHOT pool 1: 723720-726407, 739080-740999 Subtraction by Bento Soares and M. Fatima Bonaldo." BASE COUNT 91 a 127 c 150 g 79 t ORIGIN 1 ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca tgggcgaccc 61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc 121 cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc ccaggaggtg 181 tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct ggccggaggt 241 caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg ggagccaggc 301 tgctatctaa aggagtgtga ggtgcggtca cttccccact gacccgtgtc ggggtctggg 361 acaccctgag accctagctc ttctggctgc tctttaaacc tcttcatgtg gaaagtggga 421 aggaaaaaag ggagcaaaga agacaga // LOCUS AI765910 303 bp mRNA linear EST 20-DEC-1999 DEFINITION wh69a11.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2385980 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI765910 VERSION AI765910.1 GI:5232419 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 303) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 371 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..303 /db_xref="taxon:9606" /clone="IMAGE:2385980" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 54 a 95 c 108 g 46 t ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atc // LOCUS AI470866 340 bp mRNA linear EST 13-APR-1999 DEFINITION ti90c03.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2139268 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI470866 VERSION AI470866.1 GI:4332956 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 340) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1164 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 300. FEATURES Location/Qualifiers source 1..340 /db_xref="taxon:9606" /clone="IMAGE:2139268" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 59 a 99 c 124 g 52 t 6 others ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcangt tgtgtcagga ggcacctggc cgggaggtca 241 ccccctcctt ctggacccta gnnnagagtt gagggcgcct gcaccccagg ggagccaggc 301 tgctttaaag ggagtgnnga ggggggcggt cacttccgcc // LOCUS AI927886 329 bp mRNA linear EST 08-MAR-2000 DEFINITION wp03a09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2463736 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI927886 VERSION AI927886.1 GI:5663850 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 329) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 606 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..329 /db_xref="taxon:9606" /clone="IMAGE:2463736" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 59 a 89 c 119 g 57 t 5 others ORIGIN 1 ttgcttgttt cacctgctgt ttattgattg ccgggggctg cgctctcatg ggcgacccat 61 gtccccaaga gcggcactac atagaggggt tggaggaaga cggtctgaga gaagaggccg 121 cgagagccca ggtgcacaga agccccggcc ccacgggcct cacgcgcccc aggaggtgtg 181 ggcagggccc gccgcggagg gaggctgcag gtgtgtcagg aggcacctgg nnggaggtna 241 tnttctcctt ccccagagtn agcgagttga ggcgcctgca ccccagggga gccaggctgc 301 tatctaaagg agtgtgaggt gcggtcact // LOCUS AI928222 219 bp mRNA linear EST 08-MAR-2000 DEFINITION wo95e12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2463118 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI928222 VERSION AI928222.1 GI:5664186 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 219) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Trace considered overall poor quality Insert Length: 489 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..219 /db_xref="taxon:9606" /clone="IMAGE:2463118" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 37 a 71 c 77 g 34 t ORIGIN 1 acgcccccca ggaggtgtgg gcagggcccc ccccggaagg gaggctgcag gtgtgtcagg 61 aggcacctgg ccggaggtca ccccctcctt ctggaccctc agcaaagttg aggcgcctgc 121 accccagggg agccaggctg ctatctaaag gagtgtgagg tgcggtcact tccgccactg 181 gccggtgtcg gggtctggga caccctgaga ccctaagct // LOCUS AI671483 166 bp mRNA linear EST 18-MAY-1999 DEFINITION wc30g09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2316736 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AI671483 VERSION AI671483.1 GI:4851214 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 166) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Trace considered overall poor quality Seq primer: -40UP from Gibco High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..166 /db_xref="taxon:9606" /clone="IMAGE:2316736" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 35 a 51 c 53 g 27 t ORIGIN 1 ggatcacctg ctgtttattg attggcgcgg gctgggctct catgggcgac ccatgtcccc 61 aagagcggca ctacatacag gggttggagg aagacggtct gagagaagag gccgcgagag 121 cccatgtgca cagaaccccc tgccccacgg gcctatacgc gcccca // LOCUS AW299882 616 bp mRNA linear EST 18-JAN-2000 DEFINITION xs54g01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2773488 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains TAR1.t1 TAR1 repetitive element ;, mRNA sequence. ACCESSION AW299882 VERSION AW299882.1 GI:6709559 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 616) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco High quality sequence stop: 452. FEATURES Location/Qualifiers source 1..616 /db_xref="taxon:9606" /clone="IMAGE:2773488" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 117 a 180 c 216 g 100 t 3 others ORIGIN 1 gtttgtatca cctgctgttt attgattggc gggggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtcggg gtctgggaca 361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg 421 gaaaggaaaa aagggaagca aaggaagaca gacaccccgc cctcagggtg ctccccccat 481 gatgggccag ggctgcaggg gagccagccg aggcaatgcc tgcttaggac gagggagccg 541 aggtgggctt ctnctcccgc gacacggnga tggctcgctc ggcgtgngtg gcatccaggc 601 cagtctggat cttggg // LOCUS AW024358 620 bp mRNA linear EST 09-MAR-2000 DEFINITION wv03c02.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:2528450 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW024358 VERSION AW024358.1 GI:5877888 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 620) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 757 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 472. FEATURES Location/Qualifiers source 1..620 /db_xref="taxon:9606" /clone="IMAGE:2528450" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 118 a 186 c 216 g 99 t 1 others ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggagggcaac 241 cccctccttc tggaccctca gcagagttga ggcgcctgca ccccagggga gccaggctgc 301 tatctaaagg agtgtgaggt gcggtcactt ccgccactgg ccggtgtcgg ggtctgggac 361 accctgagac cctaagctct cctggctgct ctctcaaacc ctcattccat gtgagaaggt 421 ggaaaggaaa aaagggaagc aaaggaagac agacaccccg ccctcagggt gctcccccca 481 tgatgggcca gggctgcagg ggagccagcc gaggcaatgc ctgcttagga cgagggagcc 541 gaggtgggct tctcctcccg cgacacgggg atggctcgct tcgccgtggg tggcatccan 601 gcccagtctg gatcttgggg // LOCUS AW299550 484 bp mRNA linear EST 18-JAN-2000 DEFINITION xs51b12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2773151 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains TAR1.t1 TAR1 repetitive element ;, mRNA sequence. ACCESSION AW299550 VERSION AW299550.1 GI:6709227 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 484) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco High quality sequence stop: 449. FEATURES Location/Qualifiers source 1..484 /db_xref="taxon:9606" /clone="IMAGE:2773151" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 98 a 147 c 162 g 77 t ORIGIN 1 gcttgatcac ctgctgttta ttgattgccg cgggctgcgc tctcatgggc gacccatgtc 61 cccaagagcg gcactacata gaggggttgg aggaagacgg tctgagagaa gaggccgcga 121 gagcccaggt gcacagaagc cccggcccca cgggcctcac gcgccccagg aggtgtgggc 181 agggcccgcc gcggagggag gctgcaggtg tgtcaggagg cacctggccg gaggtcaccc 241 cctccttctg gaccctcagc agagttgagg cgcctgcacc ccaggggagc caggctgcta 301 tctaaaggag tgtgaggtgc ggtcacttcc gccactggcc ggtgtcgggg tctgggacac 361 cctgagaccc taagctctcc tggctgctct ctcaaaccct cattccatgt gagaaggtgg 421 aaaggaaaaa agggaagcaa aggaagacag acaccccgcc ctcagggtgc tccccccatg 481 atgg // LOCUS AW139076 417 bp mRNA linear EST 29-OCT-1999 DEFINITION UI-H-BI1-adz-d-12-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE:2718407 3', mRNA sequence. ACCESSION AW139076 VERSION AW139076.1 GI:6143394 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 417) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..417 /db_xref="taxon:9606" /clone="IMAGE:2718407" /lab_host="DH10B (Life Technologies)" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Sub3" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; The NCI_CGAP_Sub3 library is a subtracted library derived from the NCI_CGAP_Sub1 library, which is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4 , NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775 , 1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342 , 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806. TAG_LIB=NCI_CGAP_Kid3 TAG_TISSUE=kidney TAG_SEQ=AATGC" BASE COUNT 67 a 127 c 139 g 84 t ORIGIN 1 tttttttttt tttttttgct tgtatcacct gctctttatt gattgccgcg ggctgcgctc 61 tcatgggcga cccatgtccc caagagcggc actacataga ggggttggag gaagacggtc 121 tgagagaaga ggccgcgaga gcccaggtgc acagaagccc cggccccacg ggcctcacgc 181 gccccaggag gtgtgggcag ggcccgccgc ggagggaggc tgcaggtgtg tcaggaggca 241 cctggccgga ggtcaccccc tccttctgga ccctcagcag agttgaggcg cctgcacccc 301 aggggagcca ggctgctatc taaaggagtg tgaggtgcgg tcacttccgc cactggccgg 361 tgtcggggtc tgggacaccc tgagacccta agctctcctg gctgctccct cgtgccg 421 // LOCUS AW004863 397 bp mRNA linear EST 09-MAR-2000 DEFINITION wv02f10.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:2528395 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW004863 VERSION AW004863.1 GI:5853726 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 397) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 426 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..397 /db_xref="taxon:9606" /clone="IMAGE:2528395" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid3" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 68 a 125 c 137 g 67 t ORIGIN 1 tgcttgtatc acctgctctt tattgattgc cgcgggctgc ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtaggg gtctgggaca 361 ccctgagacc ctaagctctc ctggctgccc tcgtgcc // LOCUS AV653452 405 bp mRNA linear EST 15-JAN-2002 DEFINITION AV653452 GLC Homo sapiens cDNA clone GLCDLA10 3', mRNA sequence. ACCESSION AV653452 VERSION AV653452.1 GI:9874466 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 405) AUTHORS Xu,X., Huang,J., Xu,Z., Qian,B., Zhu,Z., Yan,Q., Cai,T., Zhang,X., Xiao,H., Qu,J., Liu,F., Huang,Q., Cheng,Z., Li,N., Du,J., Hu,W., Shen,K., Lu,G., Fu,G., Zhong,M., Xu,S., Gu,W., Huang,W., Zhao,X., Hu,G., Gu,J., Chen,Z. and Han,Z. TITLE Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver JOURNAL Proc. Natl. Acad. Sci. U.S.A. 98 (26), 15089-15094 (2001) MEDLINE 21625106 COMMENT Contact: Zeguang Han Chinese National Human Genome Center at Shanghai 351 Guo Shoujing Road, Zhangjiang Hi-Tech Park, Pudong, Shanghai 201203, P. R. China Tel: 86-21-50801919(ex.45) Fax: 86-21-50801922 Email: hanzg@chgc.sh.cn This clone is available at CHGC in Shanghai. FEATURES Location/Qualifiers source 1..405 /db_xref="taxon:9606" /tissue_type="corresponding non cancerous liver tissue" /clone="GLCDLA10" /lab_host="SOLR" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="GLC" /note="Vector: pBluescript sk(-); Site_1: EcoRI; Site_2: XhoI" BASE COUNT 64 a 134 c 124 g 83 t ORIGIN 1 gggaggagaa gcccacctcg gctccctcgt gctaagcagg cattgcctcg gctggctccc 61 ctgcagccct ggcccatcat ggggggagca ccctgagggc ggggtgtctg tcttcctttg 121 cttccctttt tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg 181 agagcttagg gtctcaaggt gtcccagacc ccgacaccgg ccagtggcgg aagtgaccgc 241 acctcacact cctttagata gcagcctggc tcccctgggg tgcaagcgcc tcaactctgc 301 tgagggtcca gaaggagggg gtgacctccg gccaggtgcc ttctgacaca cctgcagcct 361 cccttcgcgg cgggccctgt ccacaccttc tggggcgcgt gaggc // LOCUS AW612482 348 bp mRNA linear EST 23-MAR-2000 DEFINITION hh02g12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2953990 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW612482 VERSION AW612482.1 GI:7317668 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 348) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: image.llnl.gov/image/html/iresources.shtml Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..348 /db_xref="taxon:9606" /clone="IMAGE:2953990" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 61 a 105 c 124 g 58 t ORIGIN 1 tttgcttgta tcacctgctg tttattgatt gccgcgggct gcgctctcat gggcgaccca 61 tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag agaagaggcc 121 gcgagagccc aggtgcacag aagccccggc cccacgggcc tcacgcgccc caggaggtgt 181 gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag gaggcacctg gccggaggtc 241 accccctcct tctggaccct cagcagagtt gaggcgcctg caccccaggg gagccaggct 301 gctatctaaa ggagtgtgag gtgcggtcac ttccgccact ggccggtg // LOCUS AW242752 362 bp mRNA linear EST 14-DEC-1999 DEFINITION xm90f07.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2691493 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW242752 VERSION AW242752.1 GI:6576599 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 362) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..362 /db_xref="taxon:9606" /clone="IMAGE:2691493" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 65 a 111 c 128 g 56 t 2 others ORIGIN 1 gcttgtatca cctgctcnna taagattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg cggtcacttt ccccacttgc ccggtgtcgg ggtctgggac 361 ac // LOCUS AW139622 351 bp mRNA linear EST 30-OCT-1999 DEFINITION UI-H-BI1-adr-a-03-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE:2717477 3', mRNA sequence. ACCESSION AW139622 VERSION AW139622.1 GI:6144340 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 351) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..351 /db_xref="taxon:9606" /clone="IMAGE:2717477" /lab_host="DH10B (Life Technologies)" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Sub3" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; The NCI_CGAP_Sub3 library is a subtracted library derived from the NCI_CGAP_Sub1 library, which is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4 , NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775 , 1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342 , 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806. TAG_LIB=NCI_CGAP_Kid3 TAG_TISSUE=kidney TAG_SEQ=AATGC" BASE COUNT 59 a 102 c 121 g 69 t ORIGIN 1 tttttttttt tttttttgct tgtatcacct gctctttatt gattgccgcg ggctgcgctc 61 tcatgggcga cccatgtccc caagagcggc actacataga ggggttggag gaagacggtc 121 tgagagaaga ggccgcgaga gcccaggtgc acagaagccc cgggcccacg ggcctcacgc 181 gccccaggag gtgtgggcag ggcccgccgc ggagggaggc tgcaggtgtg tcaggaggca 241 cctggccgga ggtcaccccc tccttctgga ccctcagcag agttgaggcg cctgcacccc 301 aggggagcca ggctgctatc taaaggagtg tgaggtgcgg ccctcgtgcc g 361 // LOCUS AW771922 285 bp mRNA linear EST 04-MAY-2000 DEFINITION hn66f01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3032857 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW771922 VERSION AW771922.1 GI:7703983 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 285) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL, send email to: info@image.llnl.gov Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..285 /db_xref="taxon:9606" /clone="IMAGE:3032857" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 50 a 86 c 97 g 52 t ORIGIN 1 tttttttttg cttgtatcac ctgctcttta ttgattgccg cgggctgcgc tctcatgggc 61 gacccatgtc cccaagagcg gcactacata gaggggttgg aggaagacgg tctgagagaa 121 gaggccgcga gagcccaggt gcacagaagc cccggcccca cgggcctcac gcgccccagg 181 aggtgtgggc agggcccgcc gcggagggag gctgcaggtg tgtcaagagg cacctggccg 241 gaggtcaccc cctccttctg gaccctcagc agagttgagg cgcct // LOCUS AW269189 264 bp mRNA linear EST 03-JAN-2000 DEFINITION xs33e06.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2771458 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION AW269189 VERSION AW269189.1 GI:6656219 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 264) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..264 /db_xref="taxon:9606" /clone="IMAGE:2771458" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 49 a 85 c 96 g 34 t ORIGIN 1 attgattgcc gcgggctgcg ctctcatggg cgacccatgt ccccaagagc ggcactacat 61 agaggggttg gaggaagacg gtctgagaga agaggccgcg agagcccagg tgcacagaag 121 ccccggcccc acgggcctca cgcgccccag gaggtgtggg cagggcccgc cgcggaggga 181 ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc ccctccttct ggaccctcag 241 cagagttgag gcgcctgcac ccca // LOCUS BE048679 321 bp mRNA linear EST 08-JUN-2000 DEFINITION hr52c09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3132112 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA sequence. ACCESSION BE048679 VERSION BE048679.1 GI:8365732 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 321) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL, send email to: info@image.llnl.gov Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..321 /db_xref="taxon:9606" /clone="IMAGE:3132112" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 59 a 96 c 116 g 50 t ORIGIN 1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt 61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg 121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg 181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc 241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct 301 atctaaagga gtgtgaggtg c // LOCUS BE552434 474 bp mRNA linear EST 10-AUG-2000 DEFINITION hw26e12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3184078 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains TAR1.t1 TAR1 repetitive element ;, mRNA sequence. ACCESSION BE552434 VERSION BE552434.1 GI:9794126 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 474) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL, send email to: info@image.llnl.gov Seq primer: -40UP from Gibco High quality sequence stop: 452. FEATURES Location/Qualifiers source 1..474 /db_xref="taxon:9606" /clone="IMAGE:3184078" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NCI_CGAP_Kid11" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 95 a 138 c 155 g 86 t ORIGIN 1 tttttttttt tttgcttgta tcacctgctg tttattgatt gccgcgggct gcgctctcat 61 gggcgaccca tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag 121 agaagaggcc gcgagagccc aggtgcacag aagccccggc cccacgggcc tcacgcgccc 181 caggaggtgt gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag gaggcacctg 241 gccggaggtc accccctcct tctggaccct cagcagagtt gaggcgcctg caccccaggg 301 gagccaggct gctatctaaa ggagtgtgag gtgcggtcac ttccgccact ggccggtgtc 361 ggggtctggg acaccctgag accctaagct ctcctggctg ctctctcaaa ccctcattcc 421 atgtgagaag gtggaaagga aaaaagggaa gcaaaggaag acagacaccc cgcc 481 // LOCUS BF727324 698 bp mRNA linear EST 05-JAN-2001 DEFINITION by19h12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by19h12 5', mRNA sequence. ACCESSION BF727324 VERSION BF727324.1 GI:12043235 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 698) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 19 row: h column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..698 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by19h12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 112 a 256 c 179 g 150 t 1 others ORIGIN 1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac 61 gtgaccatcc agcacccctg gttcaagcgc accctggggc ccttctaccc cagccggctg 121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc 181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct 241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg 301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag 361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc 421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt 481 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg 541 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc 601 cctgcagccc tggcccatca tggggggagc accctgaggg cggngtgtct gtcttccttt 661 gcttcccttt tttcctttcc accttctcac atggaatg // LOCUS BF727028 629 bp mRNA linear EST 05-JAN-2001 DEFINITION by15g05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by15g05 5', mRNA sequence. ACCESSION BF727028 VERSION BF727028.1 GI:12042939 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 629) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 15 row: g column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..629 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by15g05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 105 a 234 c 164 g 126 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg 541 agaagcccac ctcggctccc tcgtcctaag caggcattgc ctcggctggc tcccctgcag 601 ccctggccca tcatgggggg agcaccctg // LOCUS BF726180 600 bp mRNA linear EST 05-JAN-2001 DEFINITION by02b10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by02b10 5', mRNA sequence. ACCESSION BF726180 VERSION BF726180.1 GI:12042091 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 600) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 02 row: b column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..600 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by02b10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 93 a 209 c 182 g 116 t ORIGIN 1 gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg aggagaagcc 61 cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg cagccctggc 121 ccatcatggg gggagcaccc tgagggcggg gtgtctgtct tcctttgctt cccttttttc 181 ctttccacct tctcacatgg aatgagggtt tgagagagca gccaggagag cttagggtct 241 cagggtgtcc cagaccccga caccggccag tggcggaagt gaccgcacct cacactcctt 301 tagatagcag cctggctccc ctggggtgca ggcgcctcaa ctctgctgag ggtccagaag 361 gagggggtga cctccggcca ggtgcctcct gacacacctg cagcctccct ccgcggcggg 421 ccctgcccac acctcctggg gcgcgtgagg cccgtggggc cggggcttct gtgcacctgg 481 gctctcgcgg cctcttctct cagaccgtct tcctccaacc cctctatgta gtgccgctct 541 tggggacatg ggtcgcccat gagagcgcag cccgcggcaa tcaataaaca gcaggtgata 601 // LOCUS BF726330 597 bp mRNA linear EST 05-JAN-2001 DEFINITION by05b01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by05b01 5', mRNA sequence. ACCESSION BF726330 VERSION BF726330.1 GI:12042241 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 597) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 05 row: b column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..597 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by05b01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 97 a 216 c 161 g 123 t ORIGIN 1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag 61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt 241 ctccccggag gacttcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca 301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct 361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgat 421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc 481 cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca ttgcctcggc 541 tggctcccct gcagccctgg cccatcatgg ggggagcacc ctgagggcgg ggtgtct 601 // LOCUS BF726422 587 bp mRNA linear EST 05-JAN-2001 DEFINITION by06d05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by06d05 5', mRNA sequence. ACCESSION BF726422 VERSION BF726422.1 GI:12042333 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 587) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 06 row: d column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..587 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by06d05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 98 a 217 c 154 g 117 t 1 others ORIGIN 1 gctgaacatg gacgtgacca tccagcaccc ctggttcaag cgcaccctgg ggcccttcta 61 ccccagccgg ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc 121 cttcctgtcg tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga 181 ctccggcatc tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa 241 gcacttctcc ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg 301 aaagcacaac gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta 361 ccgcctgccg tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat 421 gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc 481 catccncgtg tcgcgggagg agaagcccac ctcggctccc tcgtcctaag caggcattgc 541 ctcggctggc tcccctgcag ccctggccca tcatgggggg agcaccc // LOCUS BF727295 592 bp mRNA linear EST 05-JAN-2001 DEFINITION by19e10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by19e10 5', mRNA sequence. ACCESSION BF727295 VERSION BF727295.1 GI:12043206 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 592) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 19 row: e column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..592 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by19e10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 96 a 220 c 157 g 119 t ORIGIN 1 gccgctcgcg atggatgtga ccatccagca cccctggttc aagcgcaccc tggggccctt 61 ctaccccagc cggctgttcg accagttttt cggcgagggc ctttttgagt atgacctgct 121 gcccttcctg tcgtccacca tcagccccta ctaccgccag tccctcttcc gcaccgtgct 181 ggactccggc atctctgagg ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt 241 gaagcacttc tccccggagg acctcaccgt gaaggtgcag gacgactttg tggagatcca 301 cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt tccaccgccg 361 ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt ctgccgatgg 421 catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc acgccgagcg 481 agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct aagcaggcat 541 tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc tg 601 // LOCUS BF726236 576 bp mRNA linear EST 05-JAN-2001 DEFINITION by03f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by03f01 5', mRNA sequence. ACCESSION BF726236 VERSION BF726236.1 GI:12042147 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 576) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 03 row: f column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..576 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by03f01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 100 a 214 c 147 g 115 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg 541 agaagcccac ctcggctccc tcgtcctaag caggca // LOCUS BF727002 577 bp mRNA linear EST 05-JAN-2001 DEFINITION by15c11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by15c11 5', mRNA sequence. ACCESSION BF727002 VERSION BF727002.1 GI:12042913 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 577) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 15 row: c column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..577 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by15c11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 95 a 215 c 150 g 116 t 1 others ORIGIN 1 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg 61 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc 121 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct 181 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg 241 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag 301 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc 361 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt 421 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg 481 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc 541 cctgcagccc tggcccatca tggggggagc accctga // LOCUS BF726253 577 bp mRNA linear EST 05-JAN-2001 DEFINITION by03h05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by03h05 5', mRNA sequence. ACCESSION BF726253 VERSION BF726253.1 GI:12042164 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 577) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 03 row: h column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..577 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by03h05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 100 a 213 c 147 g 116 t 1 others ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc catccncgtg tcgcgggagg 541 agaagcccac ctcggctccc tcgtcctaag caggcat // LOCUS BF726679 558 bp mRNA linear EST 05-JAN-2001 DEFINITION by10d02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by10d02 5', mRNA sequence. ACCESSION BF726679 VERSION BF726679.1 GI:12042590 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 558) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 10 row: d column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..558 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by10d02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 92 a 209 c 142 g 114 t 1 others ORIGIN 1 catccagcac ccctggttca agcgcaccct gnggcccttc taccccagcc ggctgttcga 61 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat 121 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt 181 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga 241 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca 301 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt 361 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc 421 caagatccag actggcctgg atgcaaccca cgccgagcga gccatccccg tgtcgcggga 481 ggagaagccc acctcggctc cctcgtccta agcaggcatt gcctcggctg gctcccctgc 541 agccctggcc catcatgg // LOCUS BF726363 544 bp mRNA linear EST 05-JAN-2001 DEFINITION by05e10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by05e10 5', mRNA sequence. ACCESSION BF726363 VERSION BF726363.1 GI:12042274 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 544) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 05 row: e column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..544 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by05e10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 93 a 187 c 152 g 112 t ORIGIN 1 gcaccgtgct ggactccggc atctctgagg ttcgatccga ccgggacaag ttcgtcatct 61 tcctcgatgt gaagcacttc tccccggagg acctcaccgt gaaggtgcag gacgactttg 121 tggagatcca cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt 181 tccaccgccg ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt 241 ctgccgatgg catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc 301 acgccgagcg agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct 361 aagcaggcat tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc 421 tgagggcggg gtgtctgtct tcctttgctt cccttttttc ctttccacct tctcacatgg 481 aatgagggtt tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga 541 cacc // LOCUS BF726381 536 bp mRNA linear EST 05-JAN-2001 DEFINITION by05g11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by05g11 5', mRNA sequence. ACCESSION BF726381 VERSION BF726381.1 GI:12042292 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 536) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 05 row: g column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..536 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by05g11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 197 c 137 g 111 t ORIGIN 1 gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg atgtgaccat 61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca 121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag 181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg 241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct 301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga 361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga 421 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa 481 gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcggg 541 // LOCUS BF726880 527 bp mRNA linear EST 05-JAN-2001 DEFINITION by13d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by13d10 5', mRNA sequence. ACCESSION BF726880 VERSION BF726880.1 GI:12042791 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 527) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 13 row: d column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..527 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by13d10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 194 c 135 g 107 t ORIGIN 1 gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg acgtgaccat ccagcacccc 61 tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca gtttttcggc 121 gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag cccctactac 181 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg 241 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag 301 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc 361 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc 421 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact 481 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcggga // LOCUS BF726441 524 bp mRNA linear EST 05-JAN-2001 DEFINITION by06f04.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by06f04 5', mRNA sequence. ACCESSION BF726441 VERSION BF726441.1 GI:12042352 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 524) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 06 row: f column: 04 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..524 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by06f04" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 194 c 131 g 108 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc catc // LOCUS BF726358 532 bp mRNA linear EST 05-JAN-2001 DEFINITION by05d12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by05d12 5', mRNA sequence. ACCESSION BF726358 VERSION BF726358.1 GI:12042269 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 532) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 05 row: d column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..532 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by05d12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 194 c 136 g 111 t ORIGIN 1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag 61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt 241 ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca 301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct 361 gccgtccaac gtggaccagt tggccctctc ttgctccctg tctgccgatg gcatgctgac 421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc 481 cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca tt 541 // LOCUS BF726890 523 bp mRNA linear EST 05-JAN-2001 DEFINITION by13f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by13f01 5', mRNA sequence. ACCESSION BF726890 VERSION BF726890.1 GI:12042801 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 523) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 13 row: f column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..523 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by13f01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 84 a 190 c 141 g 108 t ORIGIN 1 cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca gcccctacta 61 ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc gatccgaccg 121 ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa 181 ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg acgaccacgg 241 ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg accagtcggc 301 cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca agatccagac 361 tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg agaagcccac 421 ctcggctccc tcgtcctaag caggcattgc ctcggctggc tcccctgcag ccctggccca 481 tcatgggggg agcaccctga gggcggggtg tctgtcttcc ttt // LOCUS BF726860 519 bp mRNA linear EST 05-JAN-2001 DEFINITION by13b07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by13b07 5', mRNA sequence. ACCESSION BF726860 VERSION BF726860.1 GI:12042771 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 519) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 13 row: b column: 07 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..519 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by13b07" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 90 a 191 c 131 g 107 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgag // LOCUS BF726399 536 bp mRNA linear EST 05-JAN-2001 DEFINITION by06a09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by06a09 5', mRNA sequence. ACCESSION BF726399 VERSION BF726399.1 GI:12042310 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 536) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 06 row: a column: 09 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..536 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by06a09" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 90 a 198 c 138 g 109 t 1 others ORIGIN 1 ggccgctcgc catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct 61 tctaccccag ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc 121 tgcccttcct gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc 181 tggactccgg catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg 241 tgaagcactt ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc 301 acggaaagca caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgnc 361 gctaccgcct gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg 421 gcatgctgac cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc 481 gagccatccc cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagca 541 // LOCUS BF726109 522 bp mRNA linear EST 05-JAN-2001 DEFINITION by01b12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by01b12 5', mRNA sequence. ACCESSION BF726109 VERSION BF726109.1 GI:12042020 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 522) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 01 row: b column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..522 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by01b12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 192 c 131 g 108 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca 61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc ca // LOCUS BF726854 518 bp mRNA linear EST 05-JAN-2001 DEFINITION by13a10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by13a10 5', mRNA sequence. ACCESSION BF726854 VERSION BF726854.1 GI:12042765 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 518) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 13 row: a column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..518 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by13a10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 88 a 192 c 132 g 106 t ORIGIN 1 catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc ggctgttcga 61 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat 121 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt 181 tcgatccgac cgggacaagt tcgtcatctt tctcgatgtg aagcacttct ccccggagga 241 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca 301 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt 361 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc 421 caagatccag actggcctgg atgccaccca cgccgagcga gccatccccg tgtcgcggga 481 ggagaagccc acctcggctc cctcgtccta agcaggca // LOCUS BF727140 519 bp mRNA linear EST 05-JAN-2001 DEFINITION by17d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by17d03 5', mRNA sequence. ACCESSION BF727140 VERSION BF727140.1 GI:12043051 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 519) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 17 row: d column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..519 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by17d03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 90 a 191 c 131 g 107 t ORIGIN 1 gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg atgtgaccat 61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca 121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag 181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg 241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct 301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga 361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga 421 ccagtcggcc ctctctcgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa 481 gatccagact ggcctggatg ccacccacgc cgagcgagc // LOCUS BF727010 516 bp mRNA linear EST 05-JAN-2001 DEFINITION by15d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by15d10 5', mRNA sequence. ACCESSION BF727010 VERSION BF727010.1 GI:12042921 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 516) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 15 row: d column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..516 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by15d10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 90 a 190 c 128 g 107 t 1 others ORIGIN 1 ctcctgccag cctccaggtc cccgtggtac caaagctgaa catggatgtg accatccagc 61 acccctggtt caagcgcacc ctgnggccct tctaccccag ccggctgttc gaccagtttt 121 tcggcgaggg cctttttgag tatgacctgc tgcccttcct gtcgtccacc atcagcccct 181 actaccgcca gtccctcttc cgcaccgtgc tggactccgg catctctgag gttcgatccg 241 accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag gacctcaccg 301 tgaaggtgca ggacgacttt gtggagatcc acggaaagca caacgagcgc caggacgacc 361 acggctacat ttcccgtgag ttccaccgcc gctaccgcct gccgtccaac gtggaccagt 421 cggccctctc ttgctccctg tctgccgatg gcatgctgac cttctgtggc cccaagatcc 481 agactggcct ggatgccacc cacgccgagc gagcca // LOCUS BF726595 522 bp mRNA linear EST 05-JAN-2001 DEFINITION by09a09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by09a09 5', mRNA sequence. ACCESSION BF726595 VERSION BF726595.1 GI:12042506 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 522) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 09 row: a column: 09 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..522 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by09a09" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 192 c 129 g 109 t 1 others ORIGIN 1 cgctgactcc tgccagcctc caggtcctcc tggtaccaaa gctgaacatg gatgtgacca 61 tccagcaccc ctggttcaag cgcaccctgn ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg 421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca 481 agatccagac tggcctggat gccacccacg ccgagcgagc ca // LOCUS BF726438 523 bp mRNA linear EST 05-JAN-2001 DEFINITION by06f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by06f01 5', mRNA sequence. ACCESSION BF726438 VERSION BF726438.1 GI:12042349 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 523) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 06 row: f column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..523 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by06f01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 89 a 192 c 139 g 103 t ORIGIN 1 cgcgagcggg agcggccatg gacgtgacca tccagcaccc ctggttcaag cgcaccctgg 61 ggcccttcta ccccagccgg ctgttcgacc agtttttcgg cgagggcctt tttgagtatg 121 acctgctgcc cttcctgtcg tccaccatca gcccctacta ccgccagtcc ctcttccgca 181 ccgtgctgga ctccggcatc tctgaggttc gatccgaccg ggacaagttc gtcatcttcc 241 tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg 301 agatccacgg aaagcacaac gagcgccagg acgaccacgg ctacatttcc cgtgagttcc 361 accgccgcta ccgcctgccg tccaacgtgg accagtcggc cctctcttgc tccctgtctg 421 ccgatggcat gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg 481 ccgagcgagc catccccgtg tcgcgggagg agaagcccac ctc // LOCUS BF726928 521 bp mRNA linear EST 05-JAN-2001 DEFINITION by14b12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by14b12 5', mRNA sequence. ACCESSION BF726928 VERSION BF726928.1 GI:12042839 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 521) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 14 row: b column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..521 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by14b12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 91 a 193 c 130 g 107 t ORIGIN 1 ccccgctgac tcctgccagc ctccaggtcc ccgtggtacc aaagctgaac atggatgtga 61 ccatccagca cccctggttc aagcgcaccc tggggccctt ctaccccagc cggctgttcg 121 accagttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat 181 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt 241 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccagagga 301 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca 361 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt 421 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc 481 caagatccag actggcctgg atgccaccca cgccgagcga g // LOCUS BF727221 506 bp mRNA linear EST 05-JAN-2001 DEFINITION by18e03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18e03 5', mRNA sequence. ACCESSION BF727221 VERSION BF727221.1 GI:12043132 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 506) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: e column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..506 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18e03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 88 a 183 c 128 g 106 t 1 others ORIGIN 1 gtggtaccaa agctgaacat ggatgtgacc atccagcacc cctggttcaa gcgcaccctg 61 nggcccttct accccagccg gctgttcgac cagtttttcg gcgagggcct ttttgagtat 121 gacctgctgc ccttcctgtc gtccaccatc agcccctact accgccagtc cctcttccgc 181 accgtgctgg actccggcat ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc 241 ctcgatgtga agcacttctc cccggaggac ctcaccgtga aggtgcagga cgactttgtg 301 gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc ccgtgagttc 361 caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct 421 gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac 481 gccgagcgag ccatccccgt gtcgcg // LOCUS BF727170 499 bp mRNA linear EST 05-JAN-2001 DEFINITION by17g05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by17g05 5', mRNA sequence. ACCESSION BF727170 VERSION BF727170.1 GI:12043081 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 499) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 17 row: g column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..499 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by17g05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 88 a 172 c 140 g 99 t ORIGIN 1 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga 61 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga 121 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa 181 gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga 241 gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc 301 cctggcccat catgggggga gcaccctgag ggcggggtgt ctgtcttcct ttgcttccct 361 tttttccttt ccaccttctc acatggaatg agggtttgag agagcagcca ggagagctta 421 gggtctcagg gtgtcccaga ccccgacacc ggccagtggc ggaagtgacc gcacctcaca 481 ctcctttaga tagcagcct // LOCUS BF727239 500 bp mRNA linear EST 05-JAN-2001 DEFINITION by18g02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18g02 5', mRNA sequence. ACCESSION BF727239 VERSION BF727239.1 GI:12043150 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 500) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: g column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..500 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18g02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 180 c 131 g 107 t 1 others ORIGIN 1 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg 61 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cagaggacct caccgtgaag 121 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc 181 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc 241 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact 301 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc 361 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat 421 catgggggga gcaccctgag ggcggngtgt ctgtcttcct ttgcttccct tttttccttt 481 ccaccttctc acatggaatg // LOCUS BF726856 492 bp mRNA linear EST 05-JAN-2001 DEFINITION by13b01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by13b01 5', mRNA sequence. ACCESSION BF726856 VERSION BF726856.1 GI:12042767 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 492) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 13 row: b column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..492 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by13b01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 78 a 174 c 140 g 100 t ORIGIN 1 acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc 61 cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct 121 tctgtggccc caagatccag actggcctgg atgccaccca cgccgagcga gccatccccg 181 tgtcgcggga ggagaagccc acctcggctc cctcgtccta agcaggcatt gcctcggctg 241 gctcccctgc agccctggcc catcatgggg ggagcaccct gagggcgggg tgtctgtctt 301 cctttgcttc ccttttttcc tttccacctt ctcacatgga atgagggttt gagagagcag 361 ccaggagagc ttagggtctc agggtgtccc agaccccgac accggccagt ggcggaagtg 421 accgcacctc acactccttt agatagcagc ctggctcccc tggggtgcag gcgcctcaac 481 tctgctgagg gt // LOCUS BF727205 503 bp mRNA linear EST 05-JAN-2001 DEFINITION by18b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18b11 5', mRNA sequence. ACCESSION BF727205 VERSION BF727205.1 GI:12043116 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 503) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: b column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..503 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18b11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 89 a 183 c 128 g 103 t ORIGIN 1 catggatgtg accatccagc acccctggtt caagcgcacc ctgaggccct tctaccccag 61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt 241 ctccccagag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca 301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct 361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac 421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc 481 cgtgtcgcgg gaggagaagc cca // LOCUS BF727264 485 bp mRNA linear EST 05-JAN-2001 DEFINITION by19b05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by19b05 5', mRNA sequence. ACCESSION BF727264 VERSION BF727264.1 GI:12043175 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 485) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 19 row: b column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..485 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by19b05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 180 c 122 g 101 t 1 others ORIGIN 1 gacgtgacca tccagcaccc ctggttcaag cgcaccctgn ggcccttcta ccccagccgg 61 ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg 121 tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc 181 tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc 241 ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac 301 gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg 361 tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc 421 tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg 481 tcgcg // LOCUS BF727252 477 bp mRNA linear EST 05-JAN-2001 DEFINITION by18h10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18h10 5', mRNA sequence. ACCESSION BF727252 VERSION BF727252.1 GI:12043163 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 477) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: h column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..477 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18h10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 176 c 118 g 101 t 1 others ORIGIN 1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac 61 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg 121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc 181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct 241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg 301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag 361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc 421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttc 481 // LOCUS BF727348 480 bp mRNA linear EST 05-JAN-2001 DEFINITION by20c08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by20c08 5', mRNA sequence. ACCESSION BF727348 VERSION BF727348.1 GI:12043259 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 480) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 20 row: c column: 08 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..480 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by20c08" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 80 a 179 c 120 g 101 t ORIGIN 1 ggccgctcgc cccgctgact cctgccagcc tccaggtccc cgtggtacca aagctgaaca 61 tggacgtgac catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc 121 ggctgttcga ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt 181 cgtccaccat cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca 241 tctctgaggt tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct 301 ccccggagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca 361 acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc 421 cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct 481 // LOCUS BF727230 486 bp mRNA linear EST 05-JAN-2001 DEFINITION by18e12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18e12 5', mRNA sequence. ACCESSION BF727230 VERSION BF727230.1 GI:12043141 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 486) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: e column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..486 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18e12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 83 a 169 c 142 g 92 t ORIGIN 1 gcgagcggcc ccccagagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac 61 ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc 121 taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc 181 atgctgacct tctgtggccc caagatccag actggcctgg atgccaccca cgccgagcga 241 gccatccccg tgtcgcggga ggagaagccc acctcggctc cctcgtccta agcaggcatt 301 gcctcggctg gctcccctgc agccctggcc catcatgggg ggagcaccct gagggcgggg 361 tgtctgtctt cctttgcttc ccttttttcc tttccacctt ctcacatgga atgagggttt 421 gagagagcag ccaggagagc ttagggtctc agggtgtccc agaccccgac accggccagt 481 ggcgga // LOCUS BF727402 479 bp mRNA linear EST 05-JAN-2001 DEFINITION by20h11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by20h11 5', mRNA sequence. ACCESSION BF727402 VERSION BF727402.1 GI:12043313 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 479) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 20 row: h column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..479 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by20h11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 82 a 179 c 118 g 100 t ORIGIN 1 gaccatccag cacccctggt tcaagcgcac cctgaggccc ttctacccca gccggctgtt 61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac 121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga 181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccaga 241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg 301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa 361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg 421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgc 481 // LOCUS BF726931 473 bp mRNA linear EST 05-JAN-2001 DEFINITION by14c03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by14c03 5', mRNA sequence. ACCESSION BF726931 VERSION BF726931.1 GI:12042842 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 473) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 14 row: c column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..473 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by14c03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 173 c 120 g 99 t ORIGIN 1 ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct accccagccg 61 gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc ccttcctgtc 121 gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg actccggcat 181 ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc ctcgatgtga agcacttctc 241 cccggaggac ctcaccgtga aggtgcagga cgactttgtg gagatccacg gaaagcacaa 301 cgagcgccag gacgaccacg gctacatttc ccgtgagttc caccgccgct accgcctgcc 361 gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct gccgatggca tgctgacctt 421 ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag cca 481 // LOCUS BF726645 489 bp mRNA linear EST 05-JAN-2001 DEFINITION by09g09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by09g09 5', mRNA sequence. ACCESSION BF726645 VERSION BF726645.1 GI:12042556 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 489) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 09 row: g column: 09 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..489 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by09g09" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 83 a 179 c 122 g 104 t 1 others ORIGIN 1 ggccgctcga gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct 61 gaacatggat gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc 121 cagccggctg ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt 181 cctgtcgtcc accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc 241 cggcatctct gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca 301 cttctcccca gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa 361 gcacaacgag cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg 421 cctgccgtcc aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct 481 gaccttctg // LOCUS BF727399 474 bp mRNA linear EST 05-JAN-2001 DEFINITION by20h07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by20h07 5', mRNA sequence. ACCESSION BF727399 VERSION BF727399.1 GI:12043310 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 474) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 20 row: h column: 07 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..474 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by20h07" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 173 c 119 g 100 t 1 others ORIGIN 1 catggatgtg accatccagc acccctggtt caagcgcacc ctgnggccct tctaccccag 61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt 241 ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca 301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct 361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac 421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagc 481 // LOCUS BF726920 471 bp mRNA linear EST 05-JAN-2001 DEFINITION by14b03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by14b03 5', mRNA sequence. ACCESSION BF726920 VERSION BF726920.1 GI:12042831 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 471) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 14 row: b column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..471 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by14b03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 80 a 171 c 117 g 103 t ORIGIN 1 gctgactcct gccagcctcc aggtcctcgt ggtaccaaag ctgaacatgg acgtgaccat 61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca 121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag 181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg 241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct 301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga 361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga 421 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct g 481 // LOCUS BF727220 466 bp mRNA linear EST 05-JAN-2001 DEFINITION by18d12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18d12 5', mRNA sequence. ACCESSION BF727220 VERSION BF727220.1 GI:12043131 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 466) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: d column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..466 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18d12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 80 a 174 c 126 g 86 t ORIGIN 1 gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg actccggcat 61 ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc ctcgatgtga agcacttctc 121 cccggaggac ctcaccgtga aggtgcagga cgactttgtg gagatccacg gaaagcacaa 181 cgagcgccag gacgaccacg gctacatttc ccgtgagttc caccgccgct accgcctgcc 241 gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct gccgatggca tgctgacctt 301 ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag ccatccccgt 361 gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa gcaggcattg cctcggctgg 421 ctcccctgca gccctggccc atcatggggg gagcaccctg agggcg // LOCUS BF726480 462 bp mRNA linear EST 05-JAN-2001 DEFINITION by07b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by07b11 5', mRNA sequence. ACCESSION BF726480 VERSION BF726480.1 GI:12042391 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 462) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 07 row: b column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..462 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by07b11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 79 a 159 c 127 g 97 t ORIGIN 1 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag 61 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc 121 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc 181 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact 241 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc 301 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat 361 catgggggga gcaccctgag ggcggggtgt ctgtcttcct ttgcttccct tttttccttt 421 ccaccttctc acatggaatg agggtttgag agagcagcca gg // LOCUS BF727255 472 bp mRNA linear EST 05-JAN-2001 DEFINITION by19a03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by19a03 5', mRNA sequence. ACCESSION BF727255 VERSION BF727255.1 GI:12043166 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 472) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 19 row: a column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..472 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by19a03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 80 a 173 c 117 g 101 t 1 others ORIGIN 1 ggccccgctg actcctgcca gcctccaggt ccccgtggta ccaaagctga acatggatgt 61 gaccatccag cacccctggt tcaagcgcac cctgnggccc ttctacccca gccggctgtt 121 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgttgtccac 181 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga 241 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga 301 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg 361 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa 421 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga cc 481 // LOCUS BF726623 466 bp mRNA linear EST 05-JAN-2001 DEFINITION by09d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by09d10 5', mRNA sequence. ACCESSION BF726623 VERSION BF726623.1 GI:12042534 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 466) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 09 row: d column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..466 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by09d10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 74 a 158 c 137 g 96 t 1 others ORIGIN 1 caccctgagg gcggngtgtc tgtcttcctt tgcttccctt ttttcctttc caccttctca 61 catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg tgtcccagac 121 cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat agcagcctgg 181 ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg ggtgacctcc 241 ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gcgggccctg cccacacctc 301 ctggggcgcg tgaggcccgt ggggccgggg cttctgtgca cctgggctct cgcggcctct 361 tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg acatgggtcg 421 cccatgagag cacagcccgc ggcaatcaat aaacagcagg tgatac // LOCUS BF727464 460 bp mRNA linear EST 05-JAN-2001 DEFINITION by21h03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by21h03 5', mRNA sequence. ACCESSION BF727464 VERSION BF727464.1 GI:12043375 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 460) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 21 row: h column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..460 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by21h03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 79 a 170 c 126 g 85 t ORIGIN 1 tcagccccta ctaccgccag tccctcttcc gcaccgtgct ggactccggc atctctgagg 61 ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccagagg 121 acctcaccgt gaaggtgcag gacgactttg tggagatcca cggaaagcac aacgagcgcc 181 aggacgacca cggctacatt tcccgtgagt tccaccgccg ctaccgcctg ccgtccaacg 241 tggaccagtc ggccctctct tgctccctgt ctgccgatgg catgctgacc ttctgtggcc 301 ccaagatcca gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg 361 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg 421 cagccctggc ccatcatggg gggagcaccc tgagggcggg // LOCUS BF726725 457 bp mRNA linear EST 05-JAN-2001 DEFINITION by11a06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by11a06 5', mRNA sequence. ACCESSION BF726725 VERSION BF726725.1 GI:12042636 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 457) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 11 row: a column: 06 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..457 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by11a06" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 79 a 166 c 114 g 98 t ORIGIN 1 tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca tccagcaccc 61 ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc agtttttcgg 121 cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca gcccctacta 181 ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc gatccgaccg 241 ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa 301 ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg acgaccacgg 361 ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg accagtcggc 421 cctctcttgc tccctgtctg ccgatggcat gctgacc // LOCUS BF726315 466 bp mRNA linear EST 05-JAN-2001 DEFINITION by04g12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by04g12 5', mRNA sequence. ACCESSION BF726315 VERSION BF726315.1 GI:12042226 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 466) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 04 row: g column: 12 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..466 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by04g12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 90 a 153 c 131 g 92 t ORIGIN 1 gtcttccttt gcttcccttt tttcctttcc accttctcac atggaatgag ggtttgagag 61 agcagccagg agagcttagg gtctcagggt gtcccagacc ccgacaccgg ccagtggcgg 121 aagtgaccgc acctcacact cctttagata gcagcctggc tcccctgggg tgcaggcgcc 181 tcaactctgc tgagggtcca gaaggagggg gtgacctccg gccaggtgcc tcctgacaca 241 cctgcagcct ccctccgcgg cgggccctgc ccacacctcc tggggcgcgt gaggcccgtg 301 gggccggggc ttctgtgcac ctgggctctc gcggcctctt ctctcagacc gtcttcctcc 361 aacccctcta tgtagtgccg ctcttgggga catgggtcgc ccatgagagc acagcccgcg 421 gcaatcaata aacagcaggt gatacaagca aaaaaaaaaa aaaaag // LOCUS BF726515 451 bp mRNA linear EST 05-JAN-2001 DEFINITION by07g02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by07g02 5', mRNA sequence. ACCESSION BF726515 VERSION BF726515.1 GI:12042426 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 451) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 07 row: g column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..451 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by07g02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 77 a 162 c 112 g 100 t ORIGIN 1 cgtggtacca aagctgaaca tggatgtgac catccagcac ccctggttca agcgcaccct 61 gtggcccttc taccccagcc ggctgttcga ccagtttttc ggcgagggcc tttttgagta 121 tgacctgctg cccttcctgt cgtccaccat cagcccctac taccgccagt ccctcttccg 181 caccgtgctg gactccggca tctctgaggt tcgatccgac cgggacaagt tcgtcatctt 241 cctcgatgtg aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt 301 ggagatccac ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt 361 ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc 421 tgccgatggc atgctgacct tctgtggccc c // LOCUS BF727355 438 bp mRNA linear EST 05-JAN-2001 DEFINITION by20d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by20d03 5', mRNA sequence. ACCESSION BF727355 VERSION BF727355.1 GI:12043266 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 438) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 20 row: d column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..438 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by20d03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 74 a 162 c 120 g 82 t ORIGIN 1 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg 61 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag 121 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc 181 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc 241 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact 301 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc 361 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat 421 catgggggga gcaccctg // LOCUS BF727245 432 bp mRNA linear EST 05-JAN-2001 DEFINITION by18h02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by18h02 5', mRNA sequence. ACCESSION BF727245 VERSION BF727245.1 GI:12043156 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 432) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 18 row: h column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..432 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by18h02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 74 a 146 c 128 g 84 t ORIGIN 1 cttttttcct ttccaccttc tcacatggaa tgagggtttg agagagcagc caggagagct 61 tagggtctca gggtgtccca gaccccgaca ccggccagtg gcggaagtga ccgcacctca 121 cactccttta gatagcagcc tggctcccct ggggtgcagg cgcctcaact ctgctgaggg 181 tccagaagga gggggtgacc tccggccagg tgcctcctga cacacctgca gcctccctcc 241 gcggcgggcc ctgcccacac ctcctggggc gcgtgaggcc cgtggggccg gggcttctgt 301 gcacctgggc tctcgcggcc tcttctctca gaccgtcttc ctccaacccc tctatgtagt 361 gccgctcttg gggacatggg tcgcccatga gagcacagcc cgcggcaatc aataaacagc 421 aggtgataca ag // LOCUS BF726487 428 bp mRNA linear EST 05-JAN-2001 DEFINITION by07c08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by07c08 5', mRNA sequence. ACCESSION BF726487 VERSION BF726487.1 GI:12042398 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 428) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 07 row: c column: 08 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..428 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by07c08" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 65 a 151 c 123 g 89 t ORIGIN 1 cgctaccgcc tgccgtccaa cgtggaccag tcggccctct cttgctccct gtctgccgat 61 ggcatgctga ccttctgtgg ccccaagatc cagactggcc tggatgccac ccacgccgag 121 cgagccatcc ccgtgtcgcg ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc 181 attgcctcgg ctggctcccc tgcagccctg gcccatcatg gggggagcac cctgagggcg 241 gggtgtctgt cttcctttgc ttcccttttt tcctttccac cttctcacat ggaatgaggg 301 tttgagagag cagccaggag agcttagggt ctcagggtgt cccagacccc gacaccggcc 361 agtggcggaa gtgaccgcac cttacactcc tttagatagc agcctggctc ccctggggtg 421 caggcgcc // LOCUS BF726678 412 bp mRNA linear EST 05-JAN-2001 DEFINITION by10d01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by10d01 5', mRNA sequence. ACCESSION BF726678 VERSION BF726678.1 GI:12042589 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 412) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 10 row: d column: 01 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..412 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by10d01" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 55 a 143 c 128 g 86 t ORIGIN 1 tgcctcggct ggctcccctg catccctggc ccatcatggg gggagcaccc tgagggcggg 61 gtgtctgtct tcctttgctt cccttttttc ctttccacct tctcacatgg aatgagggtt 121 tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga caccggccag 181 tggcggaagt gaccgcacct cacactcctt tagatagcag cctggctccc ctggggtgca 241 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct 301 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg 361 cccgtggggc cggggcttct gtgcacctgg gctctcgcgg cctcttctct ca 421 // LOCUS BF726250 407 bp mRNA linear EST 05-JAN-2001 DEFINITION by03h02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by03h02 5', mRNA sequence. ACCESSION BF726250 VERSION BF726250.1 GI:12042161 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 407) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 03 row: h column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..407 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by03h02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 64 a 143 c 114 g 86 t ORIGIN 1 ccgctaccgc ctgccgtcca acgtggacca gtcggccctc tcttgctccc tgtctgccga 61 tggcatgctg accttctgtg gccccaagat ccagactggc ctggatgcca cccacgccga 121 gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg gctccctcgt cctaagcagg 181 cattgcctcg gctggctccc ctgcagccct ggcccatcat ggggggagca ccctgagggc 241 ggggtgtctg tcttcctttg gtttcccttt tttcctttcc accttctcac atggaatgag 301 ggtttgagag agcagccagg agagcttagg gtctcagggt gtcccagacc ccgacaccgg 361 ccagtggcgg aagtgaccgc acctcacact cctttagata gcagcct // LOCUS BF726707 390 bp mRNA linear EST 05-JAN-2001 DEFINITION by10g06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by10g06 5', mRNA sequence. ACCESSION BF726707 VERSION BF726707.1 GI:12042618 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 390) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 10 row: g column: 06 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..390 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by10g06" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 71 a 139 c 98 g 82 t ORIGIN 1 ggccccgctg actcctgcca gcctccaggt ccccgtggta ccaaagctga acatggatgt 61 gaccatccag cacccctggt tcaagcgcac cctggggccc ttctacccca gccggctgtt 121 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac 181 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga 241 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga 301 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg 361 ccaggacgac cacggctaca tttcccgtga // LOCUS BF726516 388 bp mRNA linear EST 05-JAN-2001 DEFINITION by07g03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by07g03 5', mRNA sequence. ACCESSION BF726516 VERSION BF726516.1 GI:12042427 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 388) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 07 row: g column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..388 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by07g03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 71 a 137 c 96 g 84 t ORIGIN 1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca 61 tccagcaccc ctggttcaag cgcaccctgt ggcccttcta ccccagccgg ctgttcgacc 121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca 181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc 241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc 301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg 361 acgaccacgg ctacatttcc cgtgagtt // LOCUS BF726527 402 bp mRNA linear EST 05-JAN-2001 DEFINITION by07h08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by07h08 5', mRNA sequence. ACCESSION BF726527 VERSION BF726527.1 GI:12042438 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 402) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 07 row: h column: 08 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..402 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by07h08" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 68 a 141 c 102 g 91 t ORIGIN 1 ccttctaccc cagccggctg ttcgaccagt ttttcggcga gggccttttt gagtatgacc 61 tgctgccctt cctgtcgtcc accatcagcc cctactaccg ccagtccctc ttccgcaccg 121 tgctggactc cggcatctct gaggttcgat ccgaccggga caagtttgtc atcttcctcg 181 atgtgaagca cttctccccg gaggacctca ccgtgaaggt gcaggacgac tttgtggaga 241 tccacggaaa gcacaacgag cgccaggacg accacggcta catttcccgt gagttccacc 301 gacggtaccg cctgccgtcc aacgtggacc agtcggccct ctcttgctcc ctgtctgccg 361 atgggatgct gaccttctgt gggcccaaga tccagactgg cc // LOCUS BF727001 406 bp mRNA linear EST 05-JAN-2001 DEFINITION by15c10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by15c10 5', mRNA sequence. ACCESSION BF727001 VERSION BF727001.1 GI:12042912 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 406) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 15 row: c column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..406 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by15c10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 72 a 147 c 103 g 82 t 2 others ORIGIN 1 gcgagcggcc ggccgctcgc ctcctgccag cctccaggtc cccgtggtac caaagctgaa 61 catggacgtg accatccagc acccctggtt caagcgcacc ctgnggccct tctaccccag 121 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 181 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 241 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt 301 ctccccggag gacctcaccg tgaaggtgca ggacgactnt gtggagatcc acggaaagca 361 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccac // LOCUS BF726268 394 bp mRNA linear EST 05-JAN-2001 DEFINITION by04b05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by04b05 5', mRNA sequence. ACCESSION BF726268 VERSION BF726268.1 GI:12042179 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 394) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 04 row: b column: 05 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..394 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by04b05" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 72 a 138 c 101 g 83 t ORIGIN 1 gcgagcggcc gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggatg 61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt 121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca 181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg 241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg 301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc 361 gccaggacga ccacggctac atttcccgtg agtt // LOCUS BF726942 369 bp mRNA linear EST 05-JAN-2001 DEFINITION by14d08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by14d08 5', mRNA sequence. ACCESSION BF726942 VERSION BF726942.1 GI:12042853 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 369) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 14 row: d column: 08 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..369 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by14d08" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 60 a 127 c 107 g 75 t ORIGIN 1 tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag 61 ccatccccgt gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa gcaggcattg 121 cctcggctgg ctcccctgca gccctggccc atcatggggg gagcaccctg agggcggggt 181 gtctgtcttc ctttgcttcc cttttttcct ttccaccttc tcacatggaa tgagggtttg 241 agagagcagc caggagagct tagggtctca gggtgtccca gaccccgaca ccggccagtg 301 gcggaagtga ccgcacctca cactccttta gatagcagcc tggctcccct ggggtgcagg 361 cgcctcaac // LOCUS BF726599 338 bp mRNA linear EST 05-JAN-2001 DEFINITION by09b02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by09b02 5', mRNA sequence. ACCESSION BF726599 VERSION BF726599.1 GI:12042510 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 338) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 09 row: b column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..338 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by09b02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 61 a 118 c 83 g 74 t 2 others ORIGIN 1 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg 61 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc 121 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct 181 gaggttcgat ccgaccggga caagttcgtc atctttctcg atgtgaagca cttctccccg 241 gaggacctca ccgtgaaggt gcaggacgac tntgtggaga tccacggaaa gcacaacgag 301 cgccaggacg accacggcta catttcccgt gagttcca // LOCUS BF726132 331 bp mRNA linear EST 05-JAN-2001 DEFINITION by01e10.x1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by01e10 3', mRNA sequence. ACCESSION BF726132 VERSION BF726132.1 GI:12042043 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 331) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 01 row: e column: 10 Seq primer: -21M13 forward primer (ABI). FEATURES Location/Qualifiers source 1..331 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by01e10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 58 a 95 c 113 g 65 t ORIGIN 1 cttttttttt ttttttgctt gtatcacctg ctgtttattg attgccgcgg gctgtgctct 61 catgggcgac ccatgtcccc aagagcggca ctacatagag gggttggagg aagacggtct 121 gagagaagag gccgcgagag cccaggtgca cagaagcccc ggccccacgg gcctcacgcg 181 ccccaggagg tgtgggcagg gcccgccgcg gagggaggct gcaggtgtgt caggaggcac 241 ctggccggag gtcaccccct ccttctggac cctcagcaga gttgaggcgc ctgcacccca 301 ggggagccag gctgctatct aaaggagtgt g // LOCUS BF726108 337 bp mRNA linear EST 05-JAN-2001 DEFINITION by01b12.x1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by01b12 3', mRNA sequence. ACCESSION BF726108 VERSION BF726108.1 GI:12042019 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 337) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 01 row: b column: 12 Seq primer: -21M13 forward primer (ABI). FEATURES Location/Qualifiers source 1..337 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by01b12" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 58 a 95 c 113 g 71 t ORIGIN 1 cttttttttt tttttttttt ttgcttgtat cacctgctgt ttattgattg ccgcgggctg 61 tgctctcatg ggcgacccat gtccccaaga gcggcactac atagaggggt tggaggaaga 121 cggtctgaga gaagaggccg cgagagccca ggtgcacaga agccccggcc ccacgggcct 181 cacgcgcccc aggaggtgtg ggcagggccc gccgcggagg gaggctgcag gtgtgtcagg 241 aggcacctgg ccggaggtca ccccctcctt ctggaccctc agcagagttg aggcgcctgc 301 accccagggg agccaggctg ctatctaaag gagtgtg // LOCUS BF726318 308 bp mRNA linear EST 05-JAN-2001 DEFINITION by04h03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by04h03 5', mRNA sequence. ACCESSION BF726318 VERSION BF726318.1 GI:12042229 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 308) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 04 row: h column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..308 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by04h03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 50 a 115 c 72 g 71 t ORIGIN 1 cgcgatctag cccagaggcc ccgctgactc ctgccagcct ccaggtcccc gtggtaccaa 61 agctgaacat ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct 121 accccagccg gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc 181 ccttcctgtc gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg 241 actccggcat ctctgaggtt cgatccgacc tggacaagtt cgtcatcttc ctcgatgtga 301 agcacttc // LOCUS BF727103 350 bp mRNA linear EST 05-JAN-2001 DEFINITION by16g06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by16g06 5', mRNA sequence. ACCESSION BF727103 VERSION BF727103.1 GI:12043014 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 350) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 16 row: g column: 06 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..350 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by16g06" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 109 a 101 c 89 g 50 t 1 others ORIGIN 1 ggtgcaggcg cctcaactct gctgagggtc cagaaggagg gggtgacctc cggccaggtg 61 cctcctgaca cacctgcagc ctccctccgc ggcgggccct gcccacacct cctggggcgc 121 gtgaggcccg tggggccggg gcttctgtgc acctgggctc tcgcggcctc ttctctcaga 181 ccgtcttcct ccaacccctc tatgtagtgc cgctcttggg gacatgggtc gcccatgaga 241 gcgcagcccg cggcaatcaa taaacagcag gtgatacang ccaaaaaaaa aaaaaaaaaa 301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag // LOCUS BF727421 312 bp mRNA linear EST 05-JAN-2001 DEFINITION by21b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by21b11 5', mRNA sequence. ACCESSION BF727421 VERSION BF727421.1 GI:12043332 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 312) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 21 row: b column: 11 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..312 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by21b11" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 78 a 99 c 86 g 49 t ORIGIN 1 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct 61 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg 121 cccgtggggc cggggcttct gtgcacctgg gctctcgcgg cctcttctct cagaccgtct 181 tcctccaacc cctctatgta gtgccgctct tggggacatg ggtcgcccat gagagcgcag 241 cccgcggcaa tcaataaaca gcaggtgata caagcaaaaa aaaaaaaaaa aaaaaaaaaa 301 aaaaaaaaaa ag // LOCUS BF726810 289 bp mRNA linear EST 05-JAN-2001 DEFINITION by12d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by12d03 5', mRNA sequence. ACCESSION BF726810 VERSION BF726810.1 GI:12042721 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 289) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 12 row: d column: 03 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..289 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by12d03" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 81 a 90 c 72 g 44 t 2 others ORIGIN 1 gtgacctccg gccaggtgcc tcctgacaca cctgcagcct ccctccgcgg cgggccctgc 61 ccacacctcc tggggcgcgt gaggcccgtg gggccggggc ttctgtgcac ctgggctctc 121 gcggcctctt ctctcagacc gtcttcctcc aacccctcta tgtagtgccg ctcttgggga 181 catgggtcgc ccatgagagc gcagcccgcg gcaatcaata aacagcaggt gatacangcn 241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagg // LOCUS BF726796 218 bp mRNA linear EST 05-JAN-2001 DEFINITION by12b02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by12b02 5', mRNA sequence. ACCESSION BF726796 VERSION BF726796.1 GI:12042707 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 218) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 12 row: b column: 02 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..218 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by12b02" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 34 a 85 c 49 g 50 t ORIGIN 1 cccagaggcc ccgctgactc ctgccagcct ccaggtcccc gtggtaccaa agctgaacat 61 ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct accccagccg 121 gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc ccttcctgtc 181 gtccaccatc agcccctact accgccagtc cctttttc // LOCUS BF726222 559 bp mRNA linear EST 05-JAN-2001 DEFINITION by03c10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by03c10 5', mRNA sequence. ACCESSION BF726222 VERSION BF726222.1 GI:12042133 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 559) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 03 row: c column: 10 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..559 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by03c10" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 87 a 188 c 161 g 123 t ORIGIN 1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag 61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct 121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg 181 catctctgag gtaagacgtg gcccgtggtg ctggcctctc ctcgctgctc agagggtggt 241 ggcctcggtg ggtggagagc gatggactct ggtcttgctc cgtcaggcag gtggcctcgt 301 cccacttcat ccccttgcag aggctgggcg agagcctgtg tccccactgc agccacgtgg 361 cagagcttcc cctggcactg gggagagggt ggacaaggga gcagcctgaa tccacctttg 421 ctttcctcca tcagctcatg acccatgtgt gctttgtaag gcaccagcca catactggaa 481 accccaagag cagcccatcc aggcatgcgt ggtggcgaat gccagctccc gggttcctct 541 ggtctcctga gtcccggag // LOCUS BF726205 155 bp mRNA linear EST 05-JAN-2001 DEFINITION by02h06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by02h06 5', mRNA sequence. ACCESSION BF726205 VERSION BF726205.1 GI:12042116 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 155) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 02 row: h column: 06 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..155 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by02h06" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 59 a 41 c 31 g 24 t ORIGIN 1 cgcggcctct tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg 61 acatgggtcg cccatgagag cgcagcccgc ggcaatcaat aaacagcagg tgatacaagc 121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaag // LOCUS BF726322 474 bp mRNA linear EST 05-JAN-2001 DEFINITION by04h07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo sapiens cDNA clone by04h07 5', mRNA sequence. ACCESSION BF726322 VERSION BF726322.1 GI:12042233 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 474) AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D. TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press COMMENT Contact: Wistow G Section on Molecular Structure and Function National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov Plate: 04 row: h column: 07 Seq primer: M13RP1 reverse primer (ABI). FEATURES Location/Qualifiers source 1..474 /db_xref="taxon:9606" /tissue_type="Lens" /clone="by04h07" /lab_host="EMDH10B" /dev_stage="Adult" /organism="Homo sapiens" /clone_lib="Human Lens cDNA (Un-normalized, unamplified): BY" /note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses from different adults (both approximately 40 years old) together yielded 20ug of total RNA and 150ng mRNA for cDNA library synthesis. A directionally cloned cDNA library in the pCMVSPORT6 vector was constructed at Life Technologies , essentially following the protocols of the SuperScript Plasmid System full details of which are contained in the manufacturer's Instruction manual (http://www.lifetech.com/). First strand synthesis was carried out using a Not I primer-adapter [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt end inserts were cloned into the Not I/EcoR V sites in the vector. EST analysis was performed on the unamplified library at the NIH Intramural Sequencing Center (NISC)." BASE COUNT 238 a 80 c 86 g 70 t ORIGIN 1 cgcggcctct tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg 61 acatgggtcg cccatgagag cacagcccgc ggcaatcaat aaacagcagg tgatacaaaa 121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaggggg ggccctttta aaaaaacccc 301 cgggggggcc aaatttttcc cccccccctt ttttttgaaa aaagggcccc ctttgggggg 361 ggtatataaa accagggcca ggccccggtt taaaaaaccc ttggggggga aaaaattttt 421 tgggggaatt tttggaaaaa accccttttt tgggggggga aaaaaatggg aaaa 481 // LOCUS BI762427 927 bp mRNA linear EST 25-SEP-2001 DEFINITION 603048814F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5189115 5', mRNA sequence. ACCESSION BI762427 VERSION BI762427.1 GI:15754005 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 927) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11472 row: k column: 04 High quality sequence stop: 809. FEATURES Location/Qualifiers source 1..927 /db_xref="taxon:9606" /clone="IMAGE:5189115" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NIH_MGC_116" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 168 a 310 c 278 g 171 t ORIGIN 1 gcaaacaacc agccggccac cagtagcatt cagcccctca gctggccctg agggttggag 61 acaggtcgag ggtcatgcct gtctgtccag gagacagtca caggcccccg aaagctctgc 121 cccacttggt gtgtgggaga agaggccggc aggtgaccga agcatctctg ttctgataac 181 cgggacccgc cctgtctctg ccaaccccag cagggacggc accctctggg cagctccaca 241 tggcacgttg gatttcaggt tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg 301 aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac 361 ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccggcgc 421 taccgctgcg tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat 481 gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc 541 catccccgtg tcgcgggagg agaagcccac ctcggctcct cgtcctaagc aggcattgcc 601 tcggctggtc ccctgcagcc cctggcccat catgggggga gcaccctgag ggcggggtgt 661 ctgtcttcct ttgcttccct tttttccttt ccaccttctc acatggaatg agggttgaga 721 gagcagcagg agagcttaag gtctcagggt gtcccagacc cgacacggca gggcggaagt 781 gaccgcacct cacactcctt tagatagcag ctggtcccct ggggtgcagg gcctcaactc 841 tgctgagggt ccggaagagg gggtgacctc cggcagggcc tccgacacac cgcggccccc 901 gcgggggggc ctgccacacc tctgggc // LOCUS BI762060 811 bp mRNA linear EST 25-SEP-2001 DEFINITION 603049231F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5189551 5', mRNA sequence. ACCESSION BI762060 VERSION BI762060.1 GI:15753638 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 811) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11473 row: m column: 08 High quality sequence stop: 746. FEATURES Location/Qualifiers source 1..811 /db_xref="taxon:9606" /clone="IMAGE:5189551" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NIH_MGC_116" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 147 a 260 c 238 g 166 t ORIGIN 1 cagctggcct gagggtttgg agacaggtcg agggtcatgc ctgtctgtcc aggagacagt 61 cacaggcccc cgaaagctct gccccacttg gtgtgtggga gaagaggccg gcaggtgacc 121 gaagcatctc tgttctgata accgggaccc gccctgtctc tgccaacccc agcagggacg 181 gcaccctctg ggcagctcca catggcacgt ttggatttca ggttcgatcc gaccgggaca 241 agttcgtcat cttcctcgat gtgaagcact tctccccgga ggacctcacc gtgaaggtgc 301 aggacgactt tgtggagatc cacggaaagc acaacgagcg ccaggacgac cacggctaca 361 tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag tcggccctct 421 cttgctccct gtctgccgat ggcattgctg accttctgtg gccccaagat ccagactggc 481 ctggatgcca cccacgccga gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg 541 gctccctcgt cctaagcagg cattgcctcg gctggctccc ctgcaagccc tgtgccatca 601 ttggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc 661 caccttctca cattggaatg aggggttgag agagcagcca ggagagctta gggtctcagg 721 ggtgtccaga cccgacacgg ccagtggcgg aagtgaacgg acctcacatc ctttagatag 781 cagcttggtc ccttggggtt caaggcgccc a // LOCUS BI765128 862 bp mRNA linear EST 25-SEP-2001 DEFINITION 603051343F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5191307 5', mRNA sequence. ACCESSION BI765128 VERSION BI765128.1 GI:15756706 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 862) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11478 row: f column: 12 High quality sequence stop: 456. FEATURES Location/Qualifiers source 1..862 /db_xref="taxon:9606" /clone="IMAGE:5191307" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NIH_MGC_116" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 209 a 282 c 271 g 100 t ORIGIN 1 cctgagggcg gggtgtctgt cttcctttgc ttcccttttt tcctttccac cttctcacag 61 tggaatgagg gttgagagag cagccaggag agcttagggt ctcagggtgt cccagacccc 121 gacaccggcc agtggcggaa gtgaccgcac ctcacactcc tttagatagc agcctggctc 181 ccctggggtg caggcgcctc aactctgctg agggtccaga aggagggggt gacctccggc 241 caggtgcctc ctgacacacc tgcagcctcc ctccgcggcg ggccctgccc acacctcctg 301 gggcgcgtga ggcccgtggg gccggggctt ctgtgcacct gggctctcgc ggcctcttct 361 ctcagaccgt cttcctccaa cccctctatg tagtgccgct cttggggaca tgggtcgccc 421 atgagagcgc agccgcggaa tcaataaaca gcaggtgact acaaggcaaa ggcaccacga 481 gacacaaaga agacagacaa caaacagaag aagaaggagc aaccacgcag cagagagccg 541 ggcagaggcg gggcccccag acagcaccgc cccccggggg acacaagtaa gaacgatggc 601 acgagaagca cgcggcagac accgcgagga acaaaatggg gagcagggag cgagaagcca 661 tagacacaga ccagagacgc cgcagagcga gccgccccgg gcgacgcccg cgcccgcacg 721 gcggcaagac aggggcacag acgagaagac gagacgagag acgggaacac acgggcgacc 781 gcgcccccgg atccgaacac cgaccaacga aggcgcgcag cagcgacgaa gacgaaaaca 841 aaagccccag cgccagacac ca // LOCUS BI761339 753 bp mRNA linear EST 25-SEP-2001 DEFINITION 603043909F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5184266 5', mRNA sequence. ACCESSION BI761339 VERSION BI761339.1 GI:15752917 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 753) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11460 row: a column: 03 High quality sequence stop: 723. FEATURES Location/Qualifiers source 1..753 /db_xref="taxon:9606" /clone="IMAGE:5184266" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NIH_MGC_116" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 138 a 255 c 207 g 153 t ORIGIN 1 cttatctata accagccacc tgaccatagc caaacaacca gccggcccac cagtagcatt 61 cagcccctca gctggccctg agggtttgga gacaggtcga gggtcatgcc tgtctgtcca 121 ggagacagtc acaggccccc gaaagctctg ccccacttgg tgtgtgggag aagaggccgg 181 caggtgaccg aagcatctct gttctgataa ccgggacccg ccctgtctct gccaacccca 241 gcagggacgg caccctctgg gcagctccac atggcacgtt tggatttcag gttcgatccg 301 accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag gacctcaccg 361 tgaaggtgca ggacgacttt gtggagatcc acgggaaagc acgaacgagc gccaggacga 421 ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca acgtggacca 481 gtcggtcctc tcttgctccc tgtctgccga tggcatgctg accttctgtg gccccaagat 541 ccagactggc ctggatgcca tccacgccga gcgagccatc cccgtgtcgc gggagggaga 601 agcccagctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc 661 tggcccatca tggggggagc acccttgatg ggcggggtgt ctgtcttcct ttgcttcccg 721 ttttgacctt tccaccttct cacattggaa ctg // LOCUS BM924704 1171 bp mRNA linear EST 12-MAR-2002 DEFINITION AGENCOURT_6653675 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5761132 5', mRNA sequence. ACCESSION BM924704 VERSION BM924704.1 GI:19375083 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1171) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Agencourt Bioscience Corporation Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM12809 row: e column: 05 High quality sequence start: 8 High quality sequence stop: 760. FEATURES Location/Qualifiers source 1..1171 /db_xref="taxon:9606" /clone="IMAGE:5761132" /lab_host="DH10B" /organism="Homo sapiens" /clone_lib="NIH_MGC_116" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 230 a 394 c 323 g 222 t 2 others ORIGIN 1 gtccggaatt ctccggatgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc 61 cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg 121 agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt 181 ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct 241 gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt 301 cgcgggagga gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct 361 cccctgcagc cctggcccat catgggggga gcaccctgag ggcggggtgt ctgtcttcct 421 ttgcttccct tttttccttt ccaccttctc acatggaatg agggtttgag agagcagcca 481 ggagagctta gggtctcagg gtgtcccaga ccccgacacc ggccagtggc ggaagtgacc 541 gcacctcaca ctcctttaga tagcagcctg gctcccctgg ggtgcaggcg cctcaactct 601 gctgagggtc cagaaggagg gggtgacctc cggccaggtg cctcctgaca cacctgcagc 661 ctccctccgc ggcgggccct gcccacacct cctggggcgc gtgaggcccg tggggccggg 721 gcttctgtgc acctgggctc tcgcggcctc ttctctcaga ccgtcttcct ccaacccctc 781 tatgtagtgc cgctcttggg gacatgggtc gcccatgaga gcgcagcccg cggcaatcaa 841 taaacagcag gttgatacaa ggcaaanaga cccaacaaca aacgaaactg cgttcagctc 901 acgtccgatc ccaagcgaat ctactgcccc actcctttat agcancaaag gaggggcggc 961 cccctttaaa aaatcccccc agaggggggc ccagacttta acgggaaacc ccggttttct 1021 ttggaacaaa agggggcccc tcataaggag agcgcgtata ttaaaacaag ggaacgcggc 1081 cccgccgttc tttatacaaa ccccgcggca tggggggaaa accatccttt gcccttgcga 1141 gacactcttt cgcggagaaa gacgcccctc c // LOCUS BM679127 810 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-ahy-o-03-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-o-03-0-UI 3', mRNA sequence. ACCESSION BM679127 VERSION BM679127.1 GI:18989023 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 810) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..810 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-o-03-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 152 a 231 c 276 g 149 t 2 others ORIGIN 1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct 61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt 121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg 181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc 241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc 301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg 361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc 421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc 481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg 541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg 601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg 661 cagacaggga gcaagagagg gccgactggt ccacgttgga cggcaggcgg tancggcggt 721 ggaactcacg ggaaatgtan ccgtggtcgt cctggcgctc gttgtgcttt ccgtggatct 781 ccacaaagtc gtcctgcact tcacggtgag // LOCUS BM671973 775 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-ahz-g-22-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahz-g-22-0-UI 3', mRNA sequence. ACCESSION BM671973 VERSION BM671973.1 GI:18981871 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 775) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..775 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahz-g-22-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 144 a 220 c 268 g 141 t 2 others ORIGIN 1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct 61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt 121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg 181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc 241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc 301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg 361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc 421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc 481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg 541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg 601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg 661 cagacaggga gcaagagagg gccgactggt ccacgttgga cggcaggcgg tancggcggt 721 ggaactcacg ggaaatgtan ccgtggtcgt cctggcgctc gttgtgcttt ccgtg 781 // LOCUS BM722336 724 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahx-i-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahx-i-06-0-UI 5', mRNA sequence. ACCESSION BM722336 VERSION BM722336.1 GI:19042842 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 724) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..724 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahx-i-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 120 a 259 c 189 g 155 t 1 others ORIGIN 1 ccccgctgac tcctgccagc ctccaggtcc ccgtggtacc aaagctgaac atggacgtga 61 ccatccagca cccctggttc aagcgcaccc tggggccctt ctaccccagc cggctgttcg 121 accagttttt cggcgagggc ctttttgagt atgacctgct gcccttcctg tcgtccacca 181 tcagccccta ctaccgccag tccctcttcc gcaccgtgct ggactccggc atctctgagg 241 ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccggagg 301 acctcaccgt gaaggtgcag gacgactttg tggagatcca cggaaagcac aacgagcgcc 361 aggacgacca cggctacatt tcccgtgagt tccaccgccg ctaccgcctg ccgtccaacg 421 tggaccagtc ggccctctct tgctcnctgt ctgccgatgg catgctgacc ttctgtggcc 481 ccaagatcca gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg 541 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg 601 cagccctggc ccatcatggg gggagcaccc tgagggcggg gtgtctgtct tcctttgctt 661 cccttttttc ctttccacct tctcacatgg aatgagggtt tgagagagca gccaggagag 721 ctta // LOCUS BM721044 703 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-aia-i-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-aia-i-06-0-UI 5', mRNA sequence. ACCESSION BM721044 VERSION BM721044.1 GI:19040715 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 703) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..703 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-aia-i-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 115 a 247 c 206 g 133 t 2 others ORIGIN 1 ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccnggag gacctcaccg 61 tgaaggtgca ggacgacttt ggtggagatc cacggaaagc acaacgagcg ccaggacgac 121 cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag 181 tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg ccccaagatc 241 cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgcg ggaggagaag 301 cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg ctggctcccc tgcagccctg 361 gcccatcatg gggggagcac cctgagggcg gggtgtctgt cttcctttgc ttcccttttt 421 tcctttccac cttctcacat ggaatgaggg tttgagagag cagccaggag agcttagggt 481 ctcagggtgt cccagacccc gacaccggcc agtggcggaa gtgaccgcac ctcacactcc 541 tttagatagc agcctggctc ccctggggtg caggcgcctc aactctgctg agggtccaga 601 aggagggggt gacctccggc caggtgcctc ctgacacacc tgcagcctcc ctccgcggcg 661 ggccctgccc acacctcctg nggcgcgtga ggcccgtggg gcc // LOCUS BM671946 702 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-ahz-c-10-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahz-c-10-0-UI 3', mRNA sequence. ACCESSION BM671946 VERSION BM671946.1 GI:18981844 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 702) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..702 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahz-c-10-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 134 a 202 c 241 g 124 t 1 others ORIGIN 1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct 61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt 121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg 181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc 241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc 301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg 361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc 421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc 481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg 541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg 601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg 661 cagacaggga gcangagagg gccgactggt ccacgttgga cg // LOCUS BM696617 697 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-e-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-e-20-0-UI 5', mRNA sequence. ACCESSION BM696617 VERSION BM696617.1 GI:19009875 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 697) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..697 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-e-20-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 105 a 250 c 203 g 137 t 2 others ORIGIN 1 cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt tccaccgccg 61 ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt ctgccgatgg 121 catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc acgccgagcg 181 agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct aagcaggcat 241 tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc tgagggcggg 301 gtgtctgtct tcctttgctt ccctgttttc ctttccacct tctcacatgg aatgagggtt 361 tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga caccggccag 421 tggcggaagt gaccgcacct cacactcctt tagatagcag cctggctccc ctggggtgca 481 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct 541 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg 601 cccgtggggc cggggcttct gtgcacctgn gctctcgcgg cctcttctct cagnacgtct 661 tcctcaaccc ctctatgtag tgcgctcttg gggacat // LOCUS BM696995 664 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agl-h-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agl-h-20-0-UI 5', mRNA sequence. ACCESSION BM696995 VERSION BM696995.1 GI:19010253 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 664) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..664 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agl-h-20-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 103 a 235 c 198 g 126 t 2 others ORIGIN 1 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct 61 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc 121 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg 181 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct 241 cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc ctggcccatc 301 atggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc 361 caccttctca catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg 421 tgtcccagac cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat 481 agcagcctgg ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg 541 ggtgacctcc ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gcgggccctg 601 cccacacctc ctgnggcgcg tgaggcccgt ggggccgggg cttctgtgca cctgngctct 661 cgcg // LOCUS BM706237 667 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-c-17-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-c-17-0-UI 5', mRNA sequence. ACCESSION BM706237 VERSION BM706237.1 GI:19019495 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 667) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..667 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-c-17-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 108 a 236 c 198 g 123 t 2 others ORIGIN 1 tctccccgga ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc 61 acaacgagcg ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc 121 tgccgtccaa cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga 181 ccttctgtgg ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc 241 ccgtgtcgcg ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg 301 ctggctcccc tgcagccctg gcccatcatg gggggagcac cctgagggcg gggtgtctgt 361 cttcctttgc ttcccttttt tcctttccac cttctcacat ggaatgaggg tttgagagag 421 cagccaggag agcttagggt ctcagggtgt cccagacccc gacaccggcc agtggcggaa 481 gtgaccgcac ctcacactcc tttanatanc agcctggctc ccctggggtg caggcgcctc 541 aactctgctg agggtccaaa aggagggggt gacctccggc caggtgcctc ctgacacacc 601 tgcagcctcc ctccgcggcg ggccctgccc acacctcctg gggcgcgtga ggcccgtggg 661 gccgggg // LOCUS BM706012 653 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agg-i-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agg-i-06-0-UI 5', mRNA sequence. ACCESSION BM706012 VERSION BM706012.1 GI:19019270 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 653) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..653 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agg-i-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 101 a 231 c 196 g 125 t ORIGIN 1 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc 61 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg 121 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga 181 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc 241 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg 301 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc 361 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca 421 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc 481 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc 541 tccggccagg tgcctcctga cacacctgca gcctccctcc gcggggggcc ctgcccacac 601 ctcctggggc gcgtgaggcc cgtggggccg gggcttctgt gcacctgggc tct 661 // LOCUS BM697160 661 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-i-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-i-06-0-UI 5', mRNA sequence. ACCESSION BM697160 VERSION BM697160.1 GI:19010418 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 661) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..661 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-i-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 107 a 235 c 174 g 144 t 1 others ORIGIN 1 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt 61 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca 121 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg 181 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg 241 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc 301 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca 361 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg 421 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc 481 gggaggagaa gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc 541 ctgcagccct ggcccatcat ggggggagca ccctgagggc ggngtgtctg tcttcctttg 601 cttccctttt ttcctttcac cttctcacat ggaatgaggg tttgagagag cagccaggga 661 g // LOCUS BM722793 650 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahy-n-05-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-n-05-0-UI 5', mRNA sequence. ACCESSION BM722793 VERSION BM722793.1 GI:19043627 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 650) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..650 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-n-05-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 105 a 227 c 195 g 121 t 2 others ORIGIN 1 cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc gccaggacga 61 ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca acgtggacca 121 gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg gccccaagat 181 ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc gggaggagaa 241 gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc ctgcaaccct 301 ggcccatcat ggggggagca ccctgagggc ggggtgtctg tcttcctttg cttccctttt 361 ttcctttcca ccttctcaca tggaatgagg gtttgagaga gcagccagga gagcttaggg 421 tctcagggtg tcccagaccc cgacaccggc cagtggcgga agtgaccgca cctcacactc 481 ctttagatag cagcctggct cccctggggt gcaggcgcct caactctgct gagggtccag 541 aaggaggggg tgacctccgg ncaggtgcct cctgacacac ctgcagcctc cctccgcggc 601 gggccctgcc cacacctcct gnggcgcgtg aggcccgtgg ggccggggct // LOCUS BM686748 663 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-CQ0-adq-b-11-0-UI.r1 UI-E-CQ0 Homo sapiens cDNA clone UI-E-CQ0-adq-b-11-0-UI 5', mRNA sequence. ACCESSION BM686748 VERSION BM686748.1 GI:19000006 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 663) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..663 /db_xref="taxon:9606" /tissue_type="optic nerve" /clone="UI-E-CQ0-adq-b-11-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-CQ0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-CQ0 is a cDNA library containing the following tissue(s): optic nerve. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CCATTAAGTG. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 107 a 233 c 176 g 145 t 2 others ORIGIN 1 gcacgagggg acgtgaccat ccagcacccc tggttcaagc gcaccctggg gcccttctac 61 cccagccggc tgttcgacca gtttttcggc gagggccttt ttgagtatga cctgctgccc 121 ttcctgtcgt ccaccatcag cccctactac cgccagtccc tcttccgcac cgtgctggac 181 tccggcatct ctgaggttcg atccgaccgg gacaagttcg tcatcttcct cgatgtgaag 241 cacttctccc cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga 301 aagcacaacg agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac 361 cgcctgccgt ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg 421 ctgaccttct gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc 481 atccccgtgt cgcgggagga gaagccacct cggctccctc gtcctaagca ggcattgcct 541 cggctggctc ncctgcagcc ctggcccatc atggggggag caccctgagg gcggngtgtc 601 tgtcttcctt tgctttcctt ttttcctttc caccttctca catggaatga gggtttgaga 661 gag // LOCUS BM679139 674 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-ahy-a-10-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-a-10-0-UI 3', mRNA sequence. ACCESSION BM679139 VERSION BM679139.1 GI:18989035 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 674) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..674 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-a-10-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 129 a 194 c 228 g 122 t 1 others ORIGIN 1 tttttttttt ttttttttgc gggttgcttg tatcacctgc tgtttattga ttgccgcggg 61 ctgcgctctc atgggcgacc catgtcccca agagcggcac tacataaagg ggttggagga 121 agacggtctg agagaagagg ccgcgagagc ccaggtgcac agaagccccg gccccacggg 181 cctcacgcgc cccaggaggt gtgggcaggg cccgccgcgg agggaggctg caggtgtgtc 241 aggaggcacc tggccggagg tcaccccctc cttctggacc ctcagcagag ttgaggcgcc 301 tgcaccccag gggagccagg ctgctatcta aaggagtgtg aggtgcggtc acttccgcca 361 ctggccggtg tcggggtctg ggacaccctg agaccctaag ctctcctggc tgctctctca 421 aaccctcatt ccatgtgaga aggtggaaag gaaaaaaggg aagcaaagga agacagacac 481 cccgccctca gggtgctccc cccatgatgg gccagggctg caggggagcc agccgaggca 541 atgcctgctt aagacgaggg agccgaggtg ggcttctcct cncgcgacac ggggatggct 601 cgctcggcgt gggtggcatc caggccagtc tggatcttgg ggccacagaa ggtcagcatg 661 ccatcggcag acag // LOCUS BM706270 659 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-k-09-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-k-09-0-UI 5', mRNA sequence. ACCESSION BM706270 VERSION BM706270.1 GI:19019528 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 659) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..659 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-k-09-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 108 a 241 c 175 g 134 t 1 others ORIGIN 1 cagaggcccc gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg 61 atgtgaccat ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc 121 tgttcgacca gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt 181 ccaccatcag cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct 241 ctgaggttcg atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc 301 cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg 361 agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt 421 ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct 481 gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt 541 cgcgggagga gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct 601 ccctgcagcc cttggccatc atggggggag caccctgagg gcggngtgtc tgtcttcct 661 // LOCUS BM696872 638 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agl-o-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agl-o-20-0-UI 5', mRNA sequence. ACCESSION BM696872 VERSION BM696872.1 GI:19010130 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 638) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..638 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agl-o-20-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 109 a 221 c 181 g 126 t 1 others ORIGIN 1 cgtcatcttc ctcgatgtga agcacttctc cccggaggac ctcaccgtga aggtgcagga 61 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc 121 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg 181 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga 241 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc 301 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg 361 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc 421 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtcnca 481 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc 541 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc 601 tccggccagg tgcctcctga cacacctgca gcctccct // LOCUS BM706251 631 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-g-05-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-g-05-0-UI 5', mRNA sequence. ACCESSION BM706251 VERSION BM706251.1 GI:19019509 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 631) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..631 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-g-05-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 106 a 225 c 170 g 130 t ORIGIN 1 tgacctgctg cccttcctgt cgtccaccat cagcccctac taccgccagt ccctcttccg 61 caccgtgctg gactccggca tctctgaggt tcgatccgac cgggacaagt tcgtcatctt 121 cctcgatgtg aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt 181 ggagatccac ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt 241 ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc 301 tgccgatggc atgctgacct tctgtggccc caagatccag actggcctgg atgccaccca 361 cgccgagcga gccatccccg tgtcgcggga ggagaagccc acctcggctc cctcgtccta 421 agcaggcatt gcctcggctg gctcccctgc agccctggcc catcatgggg ggagcaccct 481 gagggcgggg tgtctgtctt cctttgcttc ccttttttcc tttccacctt ctcacatgga 541 atgagggttt gagagagcag ccaggagagc ttagggtctc agggtgtccc agaccccgac 601 accggccagt ggcggaagtg accgcacctc a // LOCUS BM697066 635 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-g-03-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-g-03-0-UI 5', mRNA sequence. ACCESSION BM697066 VERSION BM697066.1 GI:19010324 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 635) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..635 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-g-03-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 99 a 233 c 160 g 141 t 2 others ORIGIN 1 gatgtgacca tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg 61 ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg 121 tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc 181 tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc 241 ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac 301 gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg 361 tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc 421 tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg 481 tcgcgggagg agaagcccac ctcggctccc tcgtcctaag caggcattgc ctcggctggc 541 tcccctgcag ccctggccca tcatggnggg agcaccctga gggcggngtg tctgtcttcc 601 tttgcttccc ttttttcctt tccaccttct cacat // LOCUS BM696651 630 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-k-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-k-24-0-UI 5', mRNA sequence. ACCESSION BM696651 VERSION BM696651.1 GI:19009909 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 630) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..630 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-k-24-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 106 a 233 c 166 g 125 t ORIGIN 1 aggccccgct gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggatg 61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt 121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca 181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg 241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg 301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc 361 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca 421 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg 481 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc 541 gggaggagaa gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc 601 ctgcagccct gggccatcat ggggggagca // LOCUS BM696679 615 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-b-21-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-b-21-0-UI 5', mRNA sequence. ACCESSION BM696679 VERSION BM696679.1 GI:19009937 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 615) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..615 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-b-21-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 99 a 220 c 179 g 117 t ORIGIN 1 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc 61 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg 121 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga 181 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc 241 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg 301 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc 361 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca 421 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc 481 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc 541 tccggccagg tgcctcctga cacacctgca gcctccctcc gcggcgggcc ctgcccacac 601 ctcctggggc gcgtg // LOCUS BM721028 642 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-aia-e-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-aia-e-06-0-UI 5', mRNA sequence. ACCESSION BM721028 VERSION BM721028.1 GI:19040678 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 642) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..642 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-aia-e-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 102 a 229 c 167 g 143 t 1 others ORIGIN 1 accatccagc acccctggtt caagcgcacc ctgggggccc ttctacccca gccggctgtt 61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac 121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga 181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga 241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg 301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa 361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg 421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagcatccc cgtgtcgcgg 481 gaggagaagc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg 541 cagccctggc ccatcatggg gggagaccct gagggcgggg tgtctgtctt cctttgcttc 601 ncttttttcc tttccacctt ctcacatgga atgagggttt ga // LOCUS BM697176 618 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-k-18-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-k-18-0-UI 5', mRNA sequence. ACCESSION BM697176 VERSION BM697176.1 GI:19010434 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 618) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..618 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-k-18-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 106 a 209 c 174 g 126 t 3 others ORIGIN 1 ccggcatctc tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc 61 acttctcccc ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa 121 agcacaacga gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc 181 gcctgccgtc caacgtggac cagtcggccc tctcttgctc cctgtctgcc gatggcatgc 241 tgaccttctg tggccccaag atccagactg gcctggatgc cacccacgcc gagcgagcca 301 tccccgtgtc gcgggaggag aagcccacct cggctccctc gtcctaagca ggcattgcct 361 cggctggctc ccctgcagcc ctggcccatc atggggggag caccctgagg gcggngtgtc 421 tgtcttcctt tgcttcnctt ttttcctttc caccttctca catggaatga gggtttgaga 481 gagcagccag gagagcttag ggtctcaggg tgtcncagac cccgacaccg gccagtggcg 541 gaagtgaccg cacctcacac tcctttagat agcagcctgg ctcccctggg gtgcaggcgc 601 ctcaactctg ctgagggt // LOCUS BM679458 622 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-aia-e-06-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-aia-e-06-0-UI 3', mRNA sequence. ACCESSION BM679458 VERSION BM679458.1 GI:18989354 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 622) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..622 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-aia-e-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 116 a 182 c 213 g 111 t ORIGIN 1 tttttttttt ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca 61 tgggcgaccc atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga 121 gagaagaggc cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc 181 ccaggaggtg tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct 241 ggccggaggt caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg 301 ggagccaggc tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt 361 cggggtctgg gacaccctga gaccctaagc tctcctggct gctctctcaa accctcattc 421 catgtgagaa ggtggaaagg aaaaaaggga agcaaaggaa gacagacacc ccgccctcag 481 ggtgctcccc ccatgatggg ccagggctgc aggggagcca gccgaggcaa tgcctgctta 541 ggacgaggga gccgaggtgg gcttctcctc tcgcgacacg gggatggctc gctcggcgtg 601 ggtggcatcc aggccagtct gg // LOCUS BM678406 616 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-EO0-ahw-c-15-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahw-c-15-0-UI 3', mRNA sequence. ACCESSION BM678406 VERSION BM678406.1 GI:18988302 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 616) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..616 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahw-c-15-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye TAG_SEQ=CGCGTATACC" BASE COUNT 116 a 182 c 210 g 108 t ORIGIN 1 tttttttttt ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca 61 tgggcgaccc atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga 121 gagaagaggc cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc 181 ccaggaggtg tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct 241 ggccggaggt caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg 301 ggagccaggc tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt 361 cggggtctgg gacaccctga gaccctaagc tctcctggct gctctctcaa accctcattc 421 catgtgagaa ggtggaaagg aaaaaaggga agcaaaggaa gacagacacc ccgccctcag 481 ggtgctcccc ccatgatggg ccagggctgc aggggagcca gccgaggcaa tgcctgctta 541 ggacgaggga gccgaggtgg gcttctcctc ccgcgacacg gggatggctc gctcggcgtg 601 ggtggcatcc aggcca // LOCUS BM696976 599 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agl-f-04-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agl-f-04-0-UI 5', mRNA sequence. ACCESSION BM696976 VERSION BM696976.1 GI:19010234 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 599) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..599 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agl-f-04-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 98 a 208 c 161 g 131 t 1 others ORIGIN 1 ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat cagcccctac 61 taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt tcgatccgac 121 cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga cctcaccgtg 181 aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca ggacgaccac 241 ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg 301 gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc caagatccag 361 actggcctgg atgccaccca cgccgagcga gccatccccg tgtcgcggga ggagaagccc 421 acctcggctc cctcgtccta agcaggcatt gcctcggctg gctcccctgc agccctggcc 481 catcatgggg ggagcaccct gagggcggng tgtctgtctt cctttgcttc ccttttttcc 541 tttccacctt ctcacatgga atgagggttt gagagagcag ccaggagagc ttagggtct 601 // LOCUS BM696670 607 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-o-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-o-16-0-UI 5', mRNA sequence. ACCESSION BM696670 VERSION BM696670.1 GI:19009928 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 607) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..607 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-o-16-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 100 a 211 c 160 g 135 t 1 others ORIGIN 1 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca 61 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg 121 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg 181 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc 241 gccaggacga ccacggctac atttcccgtg aagttccacc gccgctaccg cctgccgtcc 301 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt 361 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg 421 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc 481 cctgcagccc tggcccatca tggggggagc accctgaggg cggngtgtct gtcttccttt 541 gcttcccttt tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg 601 agagctt // LOCUS BM697101 593 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-m-13-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-m-13-0-UI 5', mRNA sequence. ACCESSION BM697101 VERSION BM697101.1 GI:19010359 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 593) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..593 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-m-13-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 93 a 218 c 159 g 123 t ORIGIN 1 cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag 61 tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc 121 ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga 181 tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc 241 accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac 301 gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac 361 cagtcggccc tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag 421 atccagactg gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag 481 aagcccacct cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc 541 ctggcccatc atggggggag caccctgagg gcggggtgtc tgtcttcctt tgc 601 // LOCUS BM705926 592 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-f-18-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-f-18-0-UI 5', mRNA sequence. ACCESSION BM705926 VERSION BM705926.1 GI:19019184 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 592) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..592 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-f-18-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 100 a 221 c 152 g 119 t ORIGIN 1 gccccgctga ctcctgccag cctccaggtc cccgtggtac caaagctgaa catggacgtg 61 accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag ccggctgttc 121 gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct gtcgtccacc 181 atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg catctctgag 241 gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag 301 gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca caacgagcgc 361 caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct gccgtccaac 421 gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac cttctgtggc 481 cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc cgtgtcgcgg 541 gaggagaagc ccacctcggc tccctcgtcc taagcaggca ttgcctcggc tg 601 // LOCUS BM696581 596 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-o-15-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-o-15-0-UI 5', mRNA sequence. ACCESSION BM696581 VERSION BM696581.1 GI:19009839 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 596) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..596 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-o-15-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 102 a 220 c 154 g 120 t ORIGIN 1 agaggccccg ctgactcctg ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga 61 tgtgaccatc cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct 121 gttcgaccag tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc 181 caccatcagc ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc 241 tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc 301 ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga 361 gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc 421 caacgtggac cagtcggccc tctcttgctc cctgtctgcc gatggcatgc tgaccttctg 481 tggccccaag atccagactg gcctggatgc cacccacgcc gagcgagcca tccccgtgtc 541 gcgggaggag aagcccacct cggctccctc gtcctaagca ggcattgcct cggctg 601 // LOCUS BM705919 598 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-d-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-d-20-0-UI 5', mRNA sequence. ACCESSION BM705919 VERSION BM705919.1 GI:19019177 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 598) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..598 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-d-20-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 90 a 208 c 181 g 117 t 2 others ORIGIN 1 tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg agaagcccac 61 ctcggctccc tcgtcctaag caggctttgc ctcggctggc tcccctgcag ccctggccca 121 tcatgggggg agcaccctga gggcggggtg tctgtcttcc tttgcttccc ttttttcctt 181 tccaccttct cacatggaat gagggtttga gagagcagcc aggagagctt agggtctcag 241 ggtgtcccag accccgacac cggccagtgg cggaagtgac cgcacctcac actcctttag 301 atagcagcct ggctcccctg gggtgcaggc gcctcaactc tgctgagggt ccagaaggag 361 ggggtgacct ccggccaggt gcctcctgac acacctgcag cctccctccg cggcgggccc 421 tgcccacacc tcctggggcg cgtgaggccc gtggggccgg ggcttctgtg cacctgggct 481 ctcgcggnct cttctctcag accgtcttcc tccancccct ctatgtagtg ccgctcttgg 541 ggacatgggt cgcccatgag agcgcagccc gcggcaatca ataaacagca ggtgatac 601 // LOCUS BM697434 585 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-p-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-p-20-0-UI 5', mRNA sequence. ACCESSION BM697434 VERSION BM697434.1 GI:19010692 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 585) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..585 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-p-20-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 102 a 199 c 170 g 114 t ORIGIN 1 tgaagcactt ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc 61 acggaaagca caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc 121 gctaccgcct gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg 181 gcatgctgac cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc 241 gagccatccc cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca 301 ttgcctcggc tggctcccct gcagccctgg cccatcatgg ggggagcacc ctgagggcgg 361 ggtgtctgtc ttcctttgct tccctttttt cctttccacc ttctcacatg gaatgagggt 421 ttgagagagc agccaggaga gcttagggtc tcagggtgtc ccagaccccg acaccggcca 481 gtggcggaag tgaccgcacc tcacactcct ttagatagca gcctggctcc cctggggtgc 541 aggcgcctca actctgctga gggtccagaa ggagggggtg acctc // LOCUS BM721893 578 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahw-c-15-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahw-c-15-0-UI 5', mRNA sequence. ACCESSION BM721893 VERSION BM721893.1 GI:19042101 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 578) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..578 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahw-c-15-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 99 a 216 c 148 g 115 t ORIGIN 1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac 61 gtgaccatcc agcacccctg gttcaagcgc accctggggc ccttctaccc cagccggctg 121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc 181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct 241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg 301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag 361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc 421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt 481 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg 541 cgggaggaga agcccacctc ggctccctcg tcctaagc // LOCUS BM706029 585 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agg-m-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agg-m-06-0-UI 5', mRNA sequence. ACCESSION BM706029 VERSION BM706029.1 GI:19019287 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 585) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..585 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agg-m-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 84 a 206 c 177 g 117 t 1 others ORIGIN 1 gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac 61 gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa 121 gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg gagcaccctg 181 agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc tcacatggaa 241 tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca gaccccgaca 301 ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc tggctcccct 361 ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc tccggccagg 421 tgcctcctga cacacctgca gcctccctcc gcggcgggcc ctgcccacac ctcctggggc 481 gcgtgaggcc cgtggggccg gggcttctgt gcacctgggc tctcgcggnc tcttctctca 541 gaccgtcttc ctccaacccc tctatgtagt ggcgctcttg gggac // LOCUS BM696799 588 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-p-02-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-p-02-0-UI 5', mRNA sequence. ACCESSION BM696799 VERSION BM696799.1 GI:19010057 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 588) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..588 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-p-02-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 101 a 218 c 150 g 118 t 1 others ORIGIN 1 aggccccgct gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggacg 61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt 121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca 181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg 241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg 301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc 361 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca 421 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg 481 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc 541 gggaggagaa gccacctcgg ntccctcgtc ctaagcaggc attgcctc // LOCUS BM722650 586 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahy-a-10-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-a-10-0-UI 5', mRNA sequence. ACCESSION BM722650 VERSION BM722650.1 GI:19043377 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 586) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..586 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-a-10-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 100 a 219 c 148 g 119 t ORIGIN 1 cccgctgact cctgccagcc tccaggtccc cgtggtacca aagctgaaca tggatgtgac 61 catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc ggctgttcga 121 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat 181 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt 241 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga 301 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca 361 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt 421 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc 481 caagatccag actggcctgg atgccacccc acgccgagcg agccatcccc gtgtcgcggg 541 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctc // LOCUS BM697368 569 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-d-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-d-06-0-UI 5', mRNA sequence. ACCESSION BM697368 VERSION BM697368.1 GI:19010626 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 569) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..569 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-d-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 95 a 211 c 148 g 114 t 1 others ORIGIN 1 gaccatccag cacccctggt tcaagcgcac cctggggccc ttctacccca gccggctgtt 61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac 121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga 181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga 241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg 301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa 361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggnatgctga ccttctgtgg 421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgcg 481 ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg ctgactcccc 541 tgcagccctg gcccatcatg gggggagca // LOCUS BM664316 583 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-DW0-agh-b-24-0-UI.s1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-b-24-0-UI 3', mRNA sequence. ACCESSION BM664316 VERSION BM664316.1 GI:18970340 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 583) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..583 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-b-24-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-DW0 TAG_TISSUE=human lens TAG_SEQ=CGATTAGCGA" BASE COUNT 111 a 172 c 193 g 106 t 1 others ORIGIN 1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct 61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt 121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg 181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc 241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc 301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg 361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcanaccctc 421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc 481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg 541 cttaggacga gggagccgag gtgggcttct cctcccgcga cac // LOCUS BM722196 564 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahy-o-03-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-o-03-0-UI 5', mRNA sequence. ACCESSION BM722196 VERSION BM722196.1 GI:19042608 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 564) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..564 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-o-03-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 99 a 194 c 156 g 113 t 2 others ORIGIN 1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca 61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg 121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc 181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga 241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga 301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc 361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt 421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg 481 gtctcagggt gtcncagacc ccgacaccgg ncagtggcgg aagtgaccgc acctcacact 541 cctttagata gcagcctggc tccc // LOCUS BM706139 580 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agg-b-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agg-b-16-0-UI 5', mRNA sequence. ACCESSION BM706139 VERSION BM706139.1 GI:19019397 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 580) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..580 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agg-b-16-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 101 a 213 c 149 g 116 t 1 others ORIGIN 1 agaggccccg ctgactcctg ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga 61 tgtgaccatc cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct 121 gttcgaccag tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc 181 caccatcagc ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc 241 tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc 301 ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga 361 gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc 421 caacgtggac cagtcggccc tctcttgctc nctgtctgcc gatggcatgc tgaccttctg 481 tggccccaag atccagactg ggctggatgc cacccacgcc gagcgagcca tccccgtgtc 541 gcgggaggag aagcccaact cggctcccct cgtcctaagc // LOCUS BM705929 560 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-f-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-f-24-0-UI 5', mRNA sequence. ACCESSION BM705929 VERSION BM705929.1 GI:19019187 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 560) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..560 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-f-24-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 99 a 192 c 156 g 112 t 1 others ORIGIN 1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca 61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg 121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc 181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga 241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga 301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc 361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt 421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg 481 gtctcagggt gtcncagacc ccgacaccgg ccagtggcgg aagtgaccgc acctcacact 541 cctttagata gcagcctggc // LOCUS BM722779 559 bp mRNA linear EST 01-MAR-2002 DEFINITION UI-E-EO0-ahy-l-01-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone UI-E-EO0-ahy-l-01-0-UI 5', mRNA sequence. ACCESSION BM722779 VERSION BM722779.1 GI:19043602 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 559) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..559 /db_xref="taxon:9606" /tissue_type="fetal eye" /clone="UI-E-EO0-ahy-l-01-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-EO0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EO0 is a cDNA library containing the following tissue(s): fetal eye. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCGTATACC. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 97 a 206 c 142 g 113 t 1 others ORIGIN 1 ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga tgtgaccatc cagcacccct 61 ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag tttttcggcg 121 agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc ccctactacc 181 gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga tccgaccggg 241 acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc accgtgaagg 301 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct 361 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc 421 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg 481 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct 541 cggntccctc gtcctaagc // LOCUS BM696667 558 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agk-o-10-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-o-10-0-UI 5', mRNA sequence. ACCESSION BM696667 VERSION BM696667.1 GI:19009925 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 558) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..558 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-o-10-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 97 a 206 c 143 g 112 t ORIGIN 1 ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga cgtgaccatc cagcacccct 61 ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag tttttcggcg 121 agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc ccctactacc 181 gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga tccgaccggg 241 acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc accgtgaagg 301 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct 361 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcgggcc 421 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg 481 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagccacctc 541 ggctccctcg tcctaagc // LOCUS BM705911 549 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agh-b-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agh-b-24-0-UI 5', mRNA sequence. ACCESSION BM705911 VERSION BM705911.1 GI:19019169 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 549) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..549 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agh-b-24-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 97 a 188 c 152 g 111 t 1 others ORIGIN 1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca 61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg 121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc 181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga 241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga 301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc 361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt 421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg 481 gtctcagggt gtcncagacc ccgacaccgg ccagtggcgg aagtgaccgc acctcacact 541 cctttagat // LOCUS BM667923 574 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-DW0-agk-o-15-0-UI.s1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agk-o-15-0-UI 3', mRNA sequence. ACCESSION BM667923 VERSION BM667923.1 GI:18975754 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 574) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..574 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agk-o-15-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-DW0 TAG_TISSUE=human lens TAG_SEQ=CGATTAGCGA" BASE COUNT 110 a 166 c 190 g 106 t 2 others ORIGIN 1 tttttttttt ttttttttgc ntgtatcacc tgctgtttat tgattgccgc gggctgtgct 61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt 121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg 181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc 241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc 301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg 361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc 421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc 481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg 541 cttangacga gggagccgag gtgggcttct cctc // LOCUS BM698588 556 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DX0-agr-e-16-0-UI.r1 UI-E-DX0 Homo sapiens cDNA clone UI-E-DX0-agr-e-16-0-UI 5', mRNA sequence. ACCESSION BM698588 VERSION BM698588.1 GI:19011846 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 556) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..556 /db_xref="taxon:9606" /tissue_type="fetal eyes" /clone="UI-E-DX0-agr-e-16-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="fetal" /organism="Homo sapiens" /clone_lib="UI-E-DX0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DX0 is a cDNA library containing the following tissue(s): fetal eyes. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCAAGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 94 a 193 c 160 g 108 t 1 others ORIGIN 1 ccacggaaag cacaacgagc gccaggacga ccacggctac atttcccgtg agttccaccg 61 ccgctaccgc ctgccgtcca acgtggacca gtcggccctc tcttgctccc tgtctgccga 121 tggcatgctg accttctgtg gcctcaagat ccagactggc ctggatgcca cccacgccga 181 gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg gctccctcgt cctaagcagg 241 cattgcctcg gctggctccc ctgcagccct ggcccatcat ggggggagca ccctgagggc 301 ggggtgtctg tcttcctttg cttccctttt ttcctttcca ccttctcaca tggaatgagg 361 gtttgagaga gcagccagga gagcttaggg tcttagggtg tcncagaccc cgacaccggc 421 cagtggcgga agtgaccgca cctcacactc ctttagatag cagcctggct cccctggggt 481 gcaggcgcct caactctgct gagggtccag aaggaggggg tgacctccgg ccaggtgcct 541 cctgacacac ctgcag // LOCUS BM697422 547 bp mRNA linear EST 28-FEB-2002 DEFINITION UI-E-DW0-agm-n-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agm-n-16-0-UI 5', mRNA sequence. ACCESSION BM697422 VERSION BM697422.1 GI:19010680 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 547) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..547 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agm-n-16-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGATTAGCGA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI)." BASE COUNT 84 a 198 c 156 g 108 t 1 others ORIGIN 1 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc 61 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg 121 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct 181 cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc ctggcccatc 241 atggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc 301 caccttctca catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg 361 tgtcccagac cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat 421 agcagcctgg ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg 481 ggtgacctcc ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gngggccctg 541 cccacac // LOCUS BM668935 571 bp mRNA linear EST 27-FEB-2002 DEFINITION UI-E-DW0-agg-i-06-0-UI.s2 UI-E-DW0 Homo sapiens cDNA clone UI-E-DW0-agg-i-06-0-UI 3', mRNA sequence. ACCESSION BM668935 VERSION BM668935.1 GI:18978832 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 571) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) MEDLINE 97044477 COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping University of Iowa 451 Eckstein Medical Research Building Iowa City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email: msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..571 /db_xref="taxon:9606" /tissue_type="lens" /clone="UI-E-DW0-agg-i-06-0-UI" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /dev_stage="adult" /organism="Homo sapiens" /clone_lib="UI-E-DW0" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-DW0 is a cDNA library containing the following tissue(s): lens. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996.