LOCUS AI271714 568 bp mRNA linear EST 28-JAN-1999
DEFINITION qj87g04.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1866486 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI271714
VERSION AI271714.1 GI:3890881
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 568)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1163 Std
Error: 0.00 Seq primer: -40UP from Gibco High quality sequence
stop: 407.
FEATURES Location/Qualifiers
source 1..568
/db_xref="taxon:9606"
/clone="IMAGE:1866486"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 112 a 174 c 189 g 91 t 2 others
ORIGIN
1 ttttgcttgt atcacctgct ctttattgat tgccgcgggc tgcgctctca tgggcgaccc
61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc
121 cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc ccaggaggtg
181 tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct ggccggaggt
241 caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg ggagccaggc
301 tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt cgggtctggg
361 acaccctgag accctaagct ctcctggctg ctctctcaaa ccctcattcc atgtgaaaag
421 gtggaaagga aaacagggaa gcaaaggaag acagacaccc cgcccctcag gtgctccccc
481 catgatgggc cagggctgca ggggagccag ccgaggcaat gcctgcttan gacgagggag
541 gccgagtggg gctcntctcc cgcgacac
//
LOCUS AA452024 549 bp mRNA linear EST 05-JUN-1997
DEFINITION zw57c10.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
IMAGE:774162 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AA452024
VERSION AA452024.1 GI:2165693
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 549)
AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
Moore,B. , Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
White,Y., Wylie ,T., Waterston,R. and Wilson,R.
TITLE WashU-Merck EST Project 1997
JOURNAL Unpublished (1997)
COMMENT Contact: Wilson RK Washington University School of Medicine 4444
Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286
1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is
available royalty-free through LLNL ; contact the IMAGE Consortium
(info@image.llnl.gov) for further information. Seq primer: -41m13
fwd. ET from Amersham High quality sequence stop: 476.
FEATURES Location/Qualifiers
source 1..549
/db_xref="taxon:9606"
/clone="IMAGE:774162"
/lab_host="DH10B"
/dev_stage="8-9 weeks"
/organism="Homo sapiens"
/clone_lib="Soares_total_fetus_Nb2HF8_9w"
/note="Vector: pT7T3D-Pac (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand
cDNA was prepared from mRNA obtained from pooled 8-9 week
(total) fetus material with a Not I - oligo(dT) primer
[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT
3']. Double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization, and was
constructed by Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 111 a 156 c 189 g 93 t
ORIGIN
1 tttttgcttg tatcacctgc tgtttattga ttgccgcggg tgcgctctca tgggcgaccc
61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc
121 cgcgagagcc taggtgcaca gaagccccgg ccccacggct tcacgcgccc caggaggtgt
181 gggcagggcg ctcgcggagg gaggctgcag gtgtgtcagg aggcacctgg ccggagtgca
241 ccccctcctt ctggaccctc agcagagttg aggcgcctgc accccagggg agccaggctg
301 ctatctaaag gagtgtgagg tgcggtcact tccgccactg gccggtgtcg gggtctggga
361 caccctgaga ccctaagctc tcctggctgc tctctcaaac cctcattcca tgtgagaagg
421 tggaaaggaa aaaagggaag caaaggaaga cagacacccc gccctcaggg tgctcccccc
481 atgatgggcc agggctgcag gggagccagc cgaggcaatt gctgcttagg acgagggaag
541 ccaggtggg
//
LOCUS AA743802 516 bp mRNA linear EST 23-JAN-1998
DEFINITION ob01b06.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1322387 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AA743802
VERSION AA743802.1 GI:2784618
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 516)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
unknown library type Insert Length: 1221 Std Error: 0.00 Seq
primer: -40m13 fwd. ET from Amersham High quality sequence stop:
481.
FEATURES Location/Qualifiers
source 1..516
/db_xref="taxon:9606"
/clone="IMAGE:1322387"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 103 a 154 c 175 g 84 t
ORIGIN
1 ttttgcttgt atcacctgct gtttattgat tgccgcgggt gcgctctcat gggcgaccca
61 tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag agaagaggcc
121 gcgagaccca ggtgcacaga agccccggcc ccacggcgtc cacgcgcccc aggaggtgtg
181 ggcagggccg cgcgcggagg gaggctgcag gtgtgtcagg aggcacctgt gccggagtca
241 ccccctcctt ctggaccctc agcagagttg aggcgcctgc accccagggg agccaggctg
301 ctatctaaag gagtgtgagg tgcggtcact tccgccactg gccggtgtcg gggtctggga
361 caccctgaga ccctaagctc tcctggctgc tctctcaaac cctcattcca tgtgagaagg
421 tggaaaggaa aaaagggaag caaaggaaga cagacacccc gccctcaggg tgctcccccc
481 atgatgggcc agggctgcag gggagccagc cgaggc
//
LOCUS AI262258 432 bp mRNA linear EST 28-JAN-1999
DEFINITION qk09h12.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1868519 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI262258
VERSION AI262258.1 GI:3870461
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 432)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 528 Std
Error: 0.00 Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..432
/db_xref="taxon:9606"
/clone="IMAGE:1868519"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 84 a 129 c 146 g 72 t 1 others
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgncggg gtctgggaca
361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg
421 gaaaggaaaa aa
//
LOCUS AA429819 455 bp mRNA linear EST 16-OCT-1997
DEFINITION zw57c10.r1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
IMAGE:774162 5' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AA429819
VERSION AA429819.1 GI:2112992
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 455)
AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
Moore,B. , Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
White,Y., Wylie ,T., Waterston,R. and Wilson,R.
TITLE WashU-Merck EST Project 1997
JOURNAL Unpublished (1997)
COMMENT Contact: Wilson RK Washington University School of Medicine 4444
Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286
1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is
available royalty-free through LLNL ; contact the IMAGE Consortium
(info@image.llnl.gov) for further information. Seq primer: -28m13
rev2 ET from Amersham High quality sequence stop: 416.
FEATURES Location/Qualifiers
source 1..455
/db_xref="taxon:9606"
/clone="IMAGE:774162"
/lab_host="DH10B"
/dev_stage="8-9 weeks"
/organism="Homo sapiens"
/clone_lib="Soares_total_fetus_Nb2HF8_9w"
/note="Vector: pT7T3D-Pac (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand
cDNA was prepared from mRNA obtained from pooled 8-9 week
(total) fetus material with a Not I - oligo(dT) primer
[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT
3']. Double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization, and was
constructed by Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 76 a 168 c 117 g 94 t
ORIGIN
1 ggcctttttg agtatgacct gctgcccttc ctgtcgtcca ccatcagccc ctactaccgc
61 cagtccctct tccgcaccgt gctggactcc ggcatctctg aggttcgatc cgaccgggac
121 aagttcgtca tcttcctcga tgtgaagcac ttctccccgg agacctcacc gtgaaggtgc
181 aggacgactt tgtggagatc cacggaaagc acaacgagcg ccaggacgac cacggctaca
241 tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag tcggccctct
301 cttgctccct gtctgccgat gcatgctgac cttctgtggc cccaagatcc agactggcct
361 ggatgccacc cacgccgagc gagccatccc cgtgtcgcgg gaggagaagc ccactcggct
421 ccctcgtcct gagcaggcat tgctcggctg gctcc
//
LOCUS AA886899 557 bp mRNA linear EST 09-JUN-1998
DEFINITION oj26h11.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1493349 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AA886899
VERSION AA886899.1 GI:3002007
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 557)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Trace considered overall
poor quality Tissue Procurement: Christopher Moskaluk, M.D.,
Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library
Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg
Lennon, Ph.D. DNA Sequencing by: Washington University Genome
Sequencing Center Clone distribution: NCI-CGAP clone distribution
information can be found through the I.M.A.G.E. Consortium/LLNL
at: www-bio.llnl.gov/bbrp/image/image.html Trace considered
overall poor quality Insert Length: 1118 Std Error: 0.00 Seq
primer: -40m13 fwd. ET from Amersham High quality sequence stop:
1.
FEATURES Location/Qualifiers
source 1..557
/db_xref="taxon:9606"
/clone="IMAGE:1493349"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 115 a 165 c 187 g 89 t 1 others
ORIGIN
1 ttgaatcacc tgctctttat tgattgccgc gggtgcgctc tcatgggcga cccatgtccc
61 caagagcggc actacataga ggggttggag gaagacggtc tgagagaaga ggccgcgaga
121 cccaaggtgc acagaagccc cggccccacg cgtccacgcg ccccaggagg tgtgggcagg
181 gccgccgcgg agggaggctg caggtgtgtc aggaggcacc tggcggaagt tcaccccctc
241 cttctggacc ctcagcagag ttgaggcgcc tgaaccccag gggagccagg ctgctatcta
301 aaggagtgtg aggtgcggtc acttccgcca ctggccggtg tcggggtctg ggacaccctg
361 agaccctaag ctctcctggc tgctctctca aaccctcatt ccatgtgaga aggtggaaag
421 gaaaacaggg aagcaaagga agacagacac cccgcccttc aggttgctcc cccatgatgg
481 gccagggctg caggggagcc accggaggaa tgctgcttan gacgaggagc cgagtggggc
541 tctctcccgc acacggg
//
LOCUS AI277590 517 bp mRNA linear EST 29-JAN-1999
DEFINITION qm55e12.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA
clone IMAGE:1892686 3' similar to gb:U05569 ALPHA CRYSTALLIN A
CHAIN (HUMAN);, mRNA sequence.
ACCESSION AI277590
VERSION AI277590.1 GI:3899858
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 517)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 803 Std Error: 0.00 Seq primer: -40UP from Gibco
High quality sequence stop: 352.
FEATURES Location/Qualifiers
source 1..517
/db_xref="taxon:9606"
/clone="IMAGE:1892686"
/lab_host="DH10B (ampicillin resistant)"
/dev_stage="two placentae: one from 8 weeks and another
from 9 weeks post conception"
/organism="Homo sapiens"
/clone_lib="Soares_placenta_8to9weeks_2NbHP8to9W"
/note="Organ: placenta; Vector: pT7T3D (Pharmacia) with a
modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer
[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGATTTTTTTTTTTTTTTTTTT
3'], double-stranded cDNA was size selected, ligated to
Eco RI adapters (Pharmacia), digested with Not I and
cloned into the Not I and Eco RI sites of a modified
pT7T3 vector (Pharmacia). Library constructed by Bento
Soares and M.Fatima Bonaldo."
BASE COUNT 108 a 134 c 179 g 95 t 1 others
ORIGIN
1 ttttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga aggtggaaag
121 gaaaaaaggg aagcaaagga agacagacac cccgccctca gggtgctccc cccatgatgg
181 gccagggctg caggggagcc agccgaggca atgcctgctt aggacgaggg agccgaggtg
241 ggcttctcct cccgcgacac ggggatggct cgctcggcgt gggtggcatc caggccagtc
301 tggatcttgg ggccacagaa ggtcagcatg ccatcggcag acagggagca agagagggcc
361 gactggtcca cgttggacgg cangcggtag cggcggtgga actcacggga aatgtagccg
421 tgtcgtcctg ccgctcgttg tgctttccgt ggatctcaca agtcgttctg cactttaccg
481 gtgagtctcc ggggagaatg cttacatcca ggaaatg
//
LOCUS AI240847 419 bp mRNA linear EST 28-JAN-1999
DEFINITION qj99e03.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1867612 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI240847
VERSION AI240847.1 GI:3836244
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 419)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 479 Std
Error: 0.00 Seq primer: -40UP from Gibco High quality sequence
stop: 366.
FEATURES Location/Qualifiers
source 1..419
/db_xref="taxon:9606"
/clone="IMAGE:1867612"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 76 a 126 c 126 g 91 t
ORIGIN
1 tttttttttt tttttttttt tttgcttgta tcacctgctg tttattgatt gccgcgggct
61 gcgctctcat gggcgaccca tgtccccaag agcggcacta cataaagggg ttggaggaaa
121 acggtctgaa aaaaaaggcc gcgagagccc aggtgcacaa aagccccggc cccacgggcc
181 tcacgccccc caggaggtgt gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag
241 gaggcacctg gccggaggtc accccctcct tctggaccct cagcaaagtt gaggcgcctg
301 caccccaggg gagccaggct gctatctaaa ggagtgtgag gtgcggtcac ttccgccact
361 ggccggtgtc gggtctgggc accctgaacc ctaactctcc tggctgtctc taaaccctc
421
//
LOCUS AA772980 326 bp mRNA linear EST 29-JAN-1998
DEFINITION ab67a07.r1 Stratagene fetal retina 937202 Homo sapiens cDNA clone
IMAGE:851988 5' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AA772980
VERSION AA772980.1 GI:2825822
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 326)
AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin
,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
White,Y., Wylie,T., Waterston,R. and Wilson,R.
TITLE WashU-NCI human EST Project
JOURNAL Unpublished (1997)
COMMENT Contact: Wilson RK Washington University School of Medicine 4444
Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286
1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is
available royalty-free through LLNL ; contact the IMAGE Consortium
(info@image.llnl.gov) for further information. Seq primer: -28m13
rev1 ET from Amersham High quality sequence stop: 56.
FEATURES Location/Qualifiers
source 1..326
/db_xref="taxon:9606"
/clone="IMAGE:851988"
/lab_host="SOLR (kanamycin resistant)"
/sex="mixed"
/organism="Homo sapiens"
/clone_lib="Stratagene fetal retina 937202"
/note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2:
XhoI; Cloned unidirectionally. Primer: Oligo dT. Pooled
retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR
Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3'
adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'"
BASE COUNT 63 a 99 c 118 g 46 t
ORIGIN
1 gaccatgtgc ccaagagcgg cactacatag aggggttgga ggaagacggt ctgagagaag
61 aggccgcgag agccgaggtg cacagaagcc ccggccccac gcgtgcacgc gccccaggag
121 gtgtgggcag ggccgccgcg aagggaggct gcaggtgtgt caggaggcac ctggccggag
181 tcaccccctc cttctggacc ctcagcagag ttgaggcgcc tgcaccccag gggagccagg
241 ctgctatcta aaggagtgtg aggtgcggtc acttccgcca ctggccggtg ttcgggtctg
301 ggacaccctg agaccctaag ctctcc
//
LOCUS AA931460 320 bp mRNA linear EST 23-JUN-1998
DEFINITION om89d02.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:1554339 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains
PTR5.b2 TAR1 repetitive element ;, mRNA sequence.
ACCESSION AA931460
VERSION AA931460.1 GI:3085846
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 320)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 2167 Std
Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham.
FEATURES Location/Qualifiers
source 1..320
/db_xref="taxon:9606"
/clone="IMAGE:1554339"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 53 a 88 c 103 g 76 t
ORIGIN
1 tttttttttt tttttttttt tttttttttt ttgcttgtat cacctgctgt ttattgattg
61 ccgcgggtgg cgctctcatg ggcgacccat gtccccaaga gcggcactac atagaggggt
121 tggaggaaga cggtctgaga gaagaggccg cgagacccat ggtgcacaga agccccggcc
181 ccacggctgc acgcgcccca ggaggtgtgg gcagggcgca cgccgaggga ggctgcaggt
241 gtgtcaggag gcacctggcc ggagtcaccc cctccttctg gaccctcagc agagttgagg
301 cgcctgcacc ccaggggagc
//
LOCUS AA490149 142 bp mRNA linear EST 05-MAR-1998
DEFINITION ab05g09.s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone
IMAGE:839968 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AA490149
VERSION AA490149.1 GI:2221024
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 142)
AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin
,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
White,Y., Wylie,T., Waterston,R. and Wilson,R.
TITLE WashU-NCI human EST Project
JOURNAL Unpublished (1997)
COMMENT Contact: Wilson RK Washington University School of Medicine 4444
Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286
1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is
available royalty-free through LLNL ; contact the IMAGE Consortium
(info@image.llnl.gov) for further information. Insert Length: 319
Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham.
FEATURES Location/Qualifiers
source 1..142
/db_xref="taxon:9606"
/clone="IMAGE:839968"
/lab_host="SOLR (kanamycin resistant)"
/sex="mixed"
/organism="Homo sapiens"
/clone_lib="Stratagene fetal retina 937202"
/note="Vector: pBluescript SK-; Site_1: EcoRI; Site_2:
XhoI; Cloned unidirectionally. Primer: Oligo dT. Pooled
retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR
Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3'
adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'"
BASE COUNT 31 a 36 c 48 g 27 t
ORIGIN
1 tgcttgtatc acctgctgtt tattgattgc cgcgggctgc gctctcatgg gcgacccatg
61 tccccaagag cggcactaca tagaggggtt ggaggaagac ggtctgagag aagaggccgc
121 gagagcccag gtgcacagaa gc
//
LOCUS AI961742 499 bp mRNA linear EST 09-MAR-2000
DEFINITION wt66f11.x1 Soares_thymus_NHFTh Homo sapiens cDNA clone
IMAGE:2512461 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AI961742
VERSION AI961742.1 GI:5754455
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 499)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 893 Std Error: 0.00 Seq primer: -40UP from Gibco
High quality sequence stop: 410.
FEATURES Location/Qualifiers
source 1..499
/db_xref="taxon:9606"
/clone="IMAGE:2512461"
/lab_host="DH10B (phage-resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="Soares_thymus_NHFTh"
/note="Organ: thymus, pooled; Vector: pT7T3D-Pac
(Pharmacia) with a modified polylinker; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCAACGTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 99 a 151 c 167 g 80 t 2 others
ORIGIN
1 tgcttgtatc acctgctgtt tattgattgc cgcgggctgc gctctcatgg gcgacccatg
61 tccccaagag cggcactaca tagaggggtt ggaggaagac ggtctgagag aagaggccgc
121 gagagcccag gtgcacagaa gccccggccc cacgggcctc acgcgcccca ggaggtgtgg
181 gcagggcccg ccgcggaggg aggctgcagg tgtgtcagga ggcacctggc cggaggtcac
241 cccctccttc tggaccctca gcagagttga ggcgcctgca ccccagggga gccaggctgc
301 tatctaaagg agtgtgaggt gcggtcactt ccgccactgg ccggtgtcgg ngtctgggac
361 accctgagac cctaagctct cctggctgct ctctcaaacc ctcattccat gtgagaaggt
421 ggaaaggaaa aaagggaagc anaggaagac agacaccccg ccctcagggt gctcccccca
481 tgatgggcca gggctgcag
//
LOCUS AI640776 496 bp mRNA linear EST 16-DEC-1999
DEFINITION wa15d09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2298161 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI640776
VERSION AI640776.1 GI:4703885
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 496)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 733 Std
Error: 0.00 Seq primer: -40UP from Gibco High quality sequence
stop: 434.
FEATURES Location/Qualifiers
source 1..496
/db_xref="taxon:9606"
/clone="IMAGE:2298161"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 105 a 150 c 161 g 80 t
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaaaagc ggcactacat aaaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacataag ccccggcccc acgggcctca cgcgccccat gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaagag gcacctgtcc ggaggtcacc
241 cactccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggacacttc cgccactggc cggagtcggg gtctgggaca
361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg
421 gaaaggaaaa aagggaagca aaggaagaca gacaccccgc cctcagggtg ctccccccat
481 gatgggccag ggctgc
//
LOCUS AI671484 393 bp mRNA linear EST 17-DEC-1999
DEFINITION wc30g10.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2316738 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI671484
VERSION AI671484.1 GI:4851215
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 393)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 489 Std
Error: 0.00 Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..393
/db_xref="taxon:9606"
/clone="IMAGE:2316738"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 67 a 122 c 137 g 67 t
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtcggg gtctgggaca
361 ccctgagacc ctaagctctc ctggctgctc tct
//
LOCUS AI472821 447 bp mRNA linear EST 13-APR-1999
DEFINITION tj70f11.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone
IMAGE:2146893 3' similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN
(HUMAN);, mRNA sequence.
ACCESSION AI472821
VERSION AI472821.1 GI:4325866
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 447)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1029 Std Error: 0.00 Seq primer: -40UP from Gibco
High quality sequence stop: 295.
FEATURES Location/Qualifiers
source 1..447
/db_xref="taxon:9606"
/clone="IMAGE:2146893"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="Soares_NSF_F8_9W_OT_PA_P_S1"
/note="Organ: pooled; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Equal amounts of plasmid DNA from five normalized
libraries were mixed, and ss circles were made in vitro.
Following HAP purification, this DNA was used as tracer
in a subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from pools of 5,000 clones made from
the same 5 libraries. The pools consisted of the
following libraries and cloneIDs: Soares NbHSF pool 1:
309384-310919, 323208-325895 Soares Nb2HP pool 1:
145032-147335, 147720-148103, 148872-149255, 15002 -
150407, 151176-152327 Soares Nb2HF8-9W pool 1:
758280-760583, 772104-774407 Soares NbHPA pool 1:
304776-306311, 320136-322823, 326280-326663 Soares NbHOT
pool 1: 723720-726407, 739080-740999 Subtraction by
Bento Soares and M. Fatima Bonaldo."
BASE COUNT 91 a 127 c 150 g 79 t
ORIGIN
1 ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca tgggcgaccc
61 atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga gagaagaggc
121 cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc ccaggaggtg
181 tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct ggccggaggt
241 caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg ggagccaggc
301 tgctatctaa aggagtgtga ggtgcggtca cttccccact gacccgtgtc ggggtctggg
361 acaccctgag accctagctc ttctggctgc tctttaaacc tcttcatgtg gaaagtggga
421 aggaaaaaag ggagcaaaga agacaga
//
LOCUS AI765910 303 bp mRNA linear EST 20-DEC-1999
DEFINITION wh69a11.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2385980 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI765910
VERSION AI765910.1 GI:5232419
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 303)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 371 Std
Error: 0.00 Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..303
/db_xref="taxon:9606"
/clone="IMAGE:2385980"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 54 a 95 c 108 g 46 t
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atc
//
LOCUS AI470866 340 bp mRNA linear EST 13-APR-1999
DEFINITION ti90c03.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2139268 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI470866
VERSION AI470866.1 GI:4332956
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 340)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1164 Std
Error: 0.00 Seq primer: -40UP from Gibco High quality sequence
stop: 300.
FEATURES Location/Qualifiers
source 1..340
/db_xref="taxon:9606"
/clone="IMAGE:2139268"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 59 a 99 c 124 g 52 t 6 others
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcangt tgtgtcagga ggcacctggc cgggaggtca
241 ccccctcctt ctggacccta gnnnagagtt gagggcgcct gcaccccagg ggagccaggc
301 tgctttaaag ggagtgnnga ggggggcggt cacttccgcc
//
LOCUS AI927886 329 bp mRNA linear EST 08-MAR-2000
DEFINITION wp03a09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2463736 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI927886
VERSION AI927886.1 GI:5663850
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 329)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 606 Std
Error: 0.00 Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..329
/db_xref="taxon:9606"
/clone="IMAGE:2463736"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 59 a 89 c 119 g 57 t 5 others
ORIGIN
1 ttgcttgttt cacctgctgt ttattgattg ccgggggctg cgctctcatg ggcgacccat
61 gtccccaaga gcggcactac atagaggggt tggaggaaga cggtctgaga gaagaggccg
121 cgagagccca ggtgcacaga agccccggcc ccacgggcct cacgcgcccc aggaggtgtg
181 ggcagggccc gccgcggagg gaggctgcag gtgtgtcagg aggcacctgg nnggaggtna
241 tnttctcctt ccccagagtn agcgagttga ggcgcctgca ccccagggga gccaggctgc
301 tatctaaagg agtgtgaggt gcggtcact
//
LOCUS AI928222 219 bp mRNA linear EST 08-MAR-2000
DEFINITION wo95e12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2463118 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI928222
VERSION AI928222.1 GI:5664186
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 219)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Trace considered overall
poor quality Insert Length: 489 Std Error: 0.00 Seq primer: -40UP
from Gibco High quality sequence stop: 1.
FEATURES Location/Qualifiers
source 1..219
/db_xref="taxon:9606"
/clone="IMAGE:2463118"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 37 a 71 c 77 g 34 t
ORIGIN
1 acgcccccca ggaggtgtgg gcagggcccc ccccggaagg gaggctgcag gtgtgtcagg
61 aggcacctgg ccggaggtca ccccctcctt ctggaccctc agcaaagttg aggcgcctgc
121 accccagggg agccaggctg ctatctaaag gagtgtgagg tgcggtcact tccgccactg
181 gccggtgtcg gggtctggga caccctgaga ccctaagct
//
LOCUS AI671483 166 bp mRNA linear EST 18-MAY-1999
DEFINITION wc30g09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2316736 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AI671483
VERSION AI671483.1 GI:4851214
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 166)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Trace considered overall
poor quality Seq primer: -40UP from Gibco High quality sequence
stop: 1.
FEATURES Location/Qualifiers
source 1..166
/db_xref="taxon:9606"
/clone="IMAGE:2316736"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 35 a 51 c 53 g 27 t
ORIGIN
1 ggatcacctg ctgtttattg attggcgcgg gctgggctct catgggcgac ccatgtcccc
61 aagagcggca ctacatacag gggttggagg aagacggtct gagagaagag gccgcgagag
121 cccatgtgca cagaaccccc tgccccacgg gcctatacgc gcccca
//
LOCUS AW299882 616 bp mRNA linear EST 18-JAN-2000
DEFINITION xs54g01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2773488 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains
TAR1.t1 TAR1 repetitive element ;, mRNA sequence.
ACCESSION AW299882
VERSION AW299882.1 GI:6709559
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 616)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from
Gibco High quality sequence stop: 452.
FEATURES Location/Qualifiers
source 1..616
/db_xref="taxon:9606"
/clone="IMAGE:2773488"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 117 a 180 c 216 g 100 t 3 others
ORIGIN
1 gtttgtatca cctgctgttt attgattggc gggggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtcggg gtctgggaca
361 ccctgagacc ctaagctctc ctggctgctc tctcaaaccc tcattccatg tgagaaggtg
421 gaaaggaaaa aagggaagca aaggaagaca gacaccccgc cctcagggtg ctccccccat
481 gatgggccag ggctgcaggg gagccagccg aggcaatgcc tgcttaggac gagggagccg
541 aggtgggctt ctnctcccgc gacacggnga tggctcgctc ggcgtgngtg gcatccaggc
601 cagtctggat cttggg
//
LOCUS AW024358 620 bp mRNA linear EST 09-MAR-2000
DEFINITION wv03c02.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:2528450 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW024358
VERSION AW024358.1 GI:5877888
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 620)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 757 Std
Error: 0.00 Seq primer: -40UP from Gibco High quality sequence
stop: 472.
FEATURES Location/Qualifiers
source 1..620
/db_xref="taxon:9606"
/clone="IMAGE:2528450"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 118 a 186 c 216 g 99 t 1 others
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggagggcaac
241 cccctccttc tggaccctca gcagagttga ggcgcctgca ccccagggga gccaggctgc
301 tatctaaagg agtgtgaggt gcggtcactt ccgccactgg ccggtgtcgg ggtctgggac
361 accctgagac cctaagctct cctggctgct ctctcaaacc ctcattccat gtgagaaggt
421 ggaaaggaaa aaagggaagc aaaggaagac agacaccccg ccctcagggt gctcccccca
481 tgatgggcca gggctgcagg ggagccagcc gaggcaatgc ctgcttagga cgagggagcc
541 gaggtgggct tctcctcccg cgacacgggg atggctcgct tcgccgtggg tggcatccan
601 gcccagtctg gatcttgggg
//
LOCUS AW299550 484 bp mRNA linear EST 18-JAN-2000
DEFINITION xs51b12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2773151 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains
TAR1.t1 TAR1 repetitive element ;, mRNA sequence.
ACCESSION AW299550
VERSION AW299550.1 GI:6709227
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 484)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from
Gibco High quality sequence stop: 449.
FEATURES Location/Qualifiers
source 1..484
/db_xref="taxon:9606"
/clone="IMAGE:2773151"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 98 a 147 c 162 g 77 t
ORIGIN
1 gcttgatcac ctgctgttta ttgattgccg cgggctgcgc tctcatgggc gacccatgtc
61 cccaagagcg gcactacata gaggggttgg aggaagacgg tctgagagaa gaggccgcga
121 gagcccaggt gcacagaagc cccggcccca cgggcctcac gcgccccagg aggtgtgggc
181 agggcccgcc gcggagggag gctgcaggtg tgtcaggagg cacctggccg gaggtcaccc
241 cctccttctg gaccctcagc agagttgagg cgcctgcacc ccaggggagc caggctgcta
301 tctaaaggag tgtgaggtgc ggtcacttcc gccactggcc ggtgtcgggg tctgggacac
361 cctgagaccc taagctctcc tggctgctct ctcaaaccct cattccatgt gagaaggtgg
421 aaaggaaaaa agggaagcaa aggaagacag acaccccgcc ctcagggtgc tccccccatg
481 atgg
//
LOCUS AW139076 417 bp mRNA linear EST 29-OCT-1999
DEFINITION UI-H-BI1-adz-d-12-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone
IMAGE:2718407 3', mRNA sequence.
ACCESSION AW139076
VERSION AW139076.1 GI:6143394
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 417)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The
sequence contained an oligo-dT track that was present in the
oligonucleotide that was used to prime the synthesis of first
strand cDNA and therefore this may represent a bonafide poly A
tail. cDNA Library Preparation: M.B. Soares Lab Clone
distribution: NCI-CGAP clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..417
/db_xref="taxon:9606"
/clone="IMAGE:2718407"
/lab_host="DH10B (Life Technologies)"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Sub3"
/note="Vector: pT7T3D-Pac (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; The
NCI_CGAP_Sub3 library is a subtracted library derived
from the NCI_CGAP_Sub1 library, which is a subtracted
library derived from BI. BI constitutes a mixture of 21
normalized or subtracted NCI_CGAP libraries:
NCI_CGAP_Co4 , NCI_CGAP_Pr22, NCI_CGAP_Pr28,
NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5,
NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11,
NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8,
NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23,
NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19,
NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21
libraries were pooled and a single-stranded DNA
preparation of the resulting mixture was used as a tracer
in a subtractive hybridization with a driver whose
composition is detailed below: NCI_CGAP_Kid3 pool 1
LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs
1322376-1323911, 1456008-1456775 , 1500552-1502855);
NCI_CGAP_Kid5 pool 1 LLAM 3338-3342 , 3722-3725,
3776-3778 (IMAGE CloneIDs 1323912-1325831,
1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1
LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs
1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1
LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs
1257096-1258631, 1469064-1470983, 1475592-1476743);
NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068
(IMAGE CloneIDs 985608-986759, 1101192-1101959,
1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653,
2871-2872 (IMAGE CloneIDs 1057416-1061255,
1144584-1145351). Subtraction was performed as previously
described [Bonaldo, Lennon & Soares (1996): Normalization
and Subtraction: Two Approaches To Facilitate Gene
Discovery. Genome Research 6, 791-806.
TAG_LIB=NCI_CGAP_Kid3 TAG_TISSUE=kidney TAG_SEQ=AATGC"
BASE COUNT 67 a 127 c 139 g 84 t
ORIGIN
1 tttttttttt tttttttgct tgtatcacct gctctttatt gattgccgcg ggctgcgctc
61 tcatgggcga cccatgtccc caagagcggc actacataga ggggttggag gaagacggtc
121 tgagagaaga ggccgcgaga gcccaggtgc acagaagccc cggccccacg ggcctcacgc
181 gccccaggag gtgtgggcag ggcccgccgc ggagggaggc tgcaggtgtg tcaggaggca
241 cctggccgga ggtcaccccc tccttctgga ccctcagcag agttgaggcg cctgcacccc
301 aggggagcca ggctgctatc taaaggagtg tgaggtgcgg tcacttccgc cactggccgg
361 tgtcggggtc tgggacaccc tgagacccta agctctcctg gctgctccct cgtgccg
421
//
LOCUS AW004863 397 bp mRNA linear EST 09-MAR-2000
DEFINITION wv02f10.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:2528395 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW004863
VERSION AW004863.1 GI:5853726
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 397)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Insert Length: 426 Std
Error: 0.00 Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..397
/db_xref="taxon:9606"
/clone="IMAGE:2528395"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid3"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer,
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not
I and Eco RI sites of the modified pT7T3 vector. mRNA
source: 2 pooled kidneys. Library went through one round
of normalization. Library constructed by Bento Soares and
M. Fatima Bonaldo. "
BASE COUNT 68 a 125 c 137 g 67 t
ORIGIN
1 tgcttgtatc acctgctctt tattgattgc cgcgggctgc ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggtcacttc cgccactggc cggtgtaggg gtctgggaca
361 ccctgagacc ctaagctctc ctggctgccc tcgtgcc
//
LOCUS AV653452 405 bp mRNA linear EST 15-JAN-2002
DEFINITION AV653452 GLC Homo sapiens cDNA clone GLCDLA10 3', mRNA sequence.
ACCESSION AV653452
VERSION AV653452.1 GI:9874466
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 405)
AUTHORS Xu,X., Huang,J., Xu,Z., Qian,B., Zhu,Z., Yan,Q., Cai,T., Zhang,X.,
Xiao,H., Qu,J., Liu,F., Huang,Q., Cheng,Z., Li,N., Du,J., Hu,W.,
Shen,K., Lu,G., Fu,G., Zhong,M., Xu,S., Gu,W., Huang,W., Zhao,X.,
Hu,G., Gu,J., Chen,Z. and Han,Z.
TITLE Insight into hepatocellular carcinogenesis at transcriptome level
by comparing gene expression profiles of hepatocellular carcinoma
with those of corresponding noncancerous liver
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 98 (26), 15089-15094 (2001)
MEDLINE 21625106
COMMENT Contact: Zeguang Han Chinese National Human Genome Center at
Shanghai 351 Guo Shoujing Road, Zhangjiang Hi-Tech Park, Pudong,
Shanghai 201203, P. R. China Tel: 86-21-50801919(ex.45) Fax:
86-21-50801922 Email: hanzg@chgc.sh.cn This clone is available at
CHGC in Shanghai.
FEATURES Location/Qualifiers
source 1..405
/db_xref="taxon:9606"
/tissue_type="corresponding non cancerous liver tissue"
/clone="GLCDLA10"
/lab_host="SOLR"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="GLC"
/note="Vector: pBluescript sk(-); Site_1: EcoRI; Site_2:
XhoI"
BASE COUNT 64 a 134 c 124 g 83 t
ORIGIN
1 gggaggagaa gcccacctcg gctccctcgt gctaagcagg cattgcctcg gctggctccc
61 ctgcagccct ggcccatcat ggggggagca ccctgagggc ggggtgtctg tcttcctttg
121 cttccctttt tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg
181 agagcttagg gtctcaaggt gtcccagacc ccgacaccgg ccagtggcgg aagtgaccgc
241 acctcacact cctttagata gcagcctggc tcccctgggg tgcaagcgcc tcaactctgc
301 tgagggtcca gaaggagggg gtgacctccg gccaggtgcc ttctgacaca cctgcagcct
361 cccttcgcgg cgggccctgt ccacaccttc tggggcgcgt gaggc
//
LOCUS AW612482 348 bp mRNA linear EST 23-MAR-2000
DEFINITION hh02g12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2953990 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW612482
VERSION AW612482.1 GI:7317668
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 348)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
image.llnl.gov/image/html/iresources.shtml Seq primer: -40UP from
Gibco.
FEATURES Location/Qualifiers
source 1..348
/db_xref="taxon:9606"
/clone="IMAGE:2953990"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 61 a 105 c 124 g 58 t
ORIGIN
1 tttgcttgta tcacctgctg tttattgatt gccgcgggct gcgctctcat gggcgaccca
61 tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag agaagaggcc
121 gcgagagccc aggtgcacag aagccccggc cccacgggcc tcacgcgccc caggaggtgt
181 gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag gaggcacctg gccggaggtc
241 accccctcct tctggaccct cagcagagtt gaggcgcctg caccccaggg gagccaggct
301 gctatctaaa ggagtgtgag gtgcggtcac ttccgccact ggccggtg
//
LOCUS AW242752 362 bp mRNA linear EST 14-DEC-1999
DEFINITION xm90f07.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2691493 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW242752
VERSION AW242752.1 GI:6576599
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 362)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from
Gibco.
FEATURES Location/Qualifiers
source 1..362
/db_xref="taxon:9606"
/clone="IMAGE:2691493"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 65 a 111 c 128 g 56 t 2 others
ORIGIN
1 gcttgtatca cctgctcnna taagattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg cggtcacttt ccccacttgc ccggtgtcgg ggtctgggac
361 ac
//
LOCUS AW139622 351 bp mRNA linear EST 30-OCT-1999
DEFINITION UI-H-BI1-adr-a-03-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone
IMAGE:2717477 3', mRNA sequence.
ACCESSION AW139622
VERSION AW139622.1 GI:6144340
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 351)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The
sequence contained an oligo-dT track that was present in the
oligonucleotide that was used to prime the synthesis of first
strand cDNA and therefore this may represent a bonafide poly A
tail. cDNA Library Preparation: M.B. Soares Lab Clone
distribution: NCI-CGAP clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..351
/db_xref="taxon:9606"
/clone="IMAGE:2717477"
/lab_host="DH10B (Life Technologies)"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Sub3"
/note="Vector: pT7T3D-Pac (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; The
NCI_CGAP_Sub3 library is a subtracted library derived
from the NCI_CGAP_Sub1 library, which is a subtracted
library derived from BI. BI constitutes a mixture of 21
normalized or subtracted NCI_CGAP libraries:
NCI_CGAP_Co4 , NCI_CGAP_Pr22, NCI_CGAP_Pr28,
NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5,
NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11,
NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8,
NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23,
NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19,
NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21
libraries were pooled and a single-stranded DNA
preparation of the resulting mixture was used as a tracer
in a subtractive hybridization with a driver whose
composition is detailed below: NCI_CGAP_Kid3 pool 1
LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs
1322376-1323911, 1456008-1456775 , 1500552-1502855);
NCI_CGAP_Kid5 pool 1 LLAM 3338-3342 , 3722-3725,
3776-3778 (IMAGE CloneIDs 1323912-1325831,
1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1
LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs
1414920-1417991, 1520904-1522439); NCI_CGAP_GC4 pool 1
LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs
1257096-1258631, 1469064-1470983, 1475592-1476743);
NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068
(IMAGE CloneIDs 985608-986759, 1101192-1101959,
1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653,
2871-2872 (IMAGE CloneIDs 1057416-1061255,
1144584-1145351). Subtraction was performed as previously
described [Bonaldo, Lennon & Soares (1996): Normalization
and Subtraction: Two Approaches To Facilitate Gene
Discovery. Genome Research 6, 791-806.
TAG_LIB=NCI_CGAP_Kid3 TAG_TISSUE=kidney TAG_SEQ=AATGC"
BASE COUNT 59 a 102 c 121 g 69 t
ORIGIN
1 tttttttttt tttttttgct tgtatcacct gctctttatt gattgccgcg ggctgcgctc
61 tcatgggcga cccatgtccc caagagcggc actacataga ggggttggag gaagacggtc
121 tgagagaaga ggccgcgaga gcccaggtgc acagaagccc cgggcccacg ggcctcacgc
181 gccccaggag gtgtgggcag ggcccgccgc ggagggaggc tgcaggtgtg tcaggaggca
241 cctggccgga ggtcaccccc tccttctgga ccctcagcag agttgaggcg cctgcacccc
301 aggggagcca ggctgctatc taaaggagtg tgaggtgcgg ccctcgtgcc g
361
//
LOCUS AW771922 285 bp mRNA linear EST 04-MAY-2000
DEFINITION hn66f01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3032857 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW771922
VERSION AW771922.1 GI:7703983
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 285)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL, send email to:
info@image.llnl.gov Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..285
/db_xref="taxon:9606"
/clone="IMAGE:3032857"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 50 a 86 c 97 g 52 t
ORIGIN
1 tttttttttg cttgtatcac ctgctcttta ttgattgccg cgggctgcgc tctcatgggc
61 gacccatgtc cccaagagcg gcactacata gaggggttgg aggaagacgg tctgagagaa
121 gaggccgcga gagcccaggt gcacagaagc cccggcccca cgggcctcac gcgccccagg
181 aggtgtgggc agggcccgcc gcggagggag gctgcaggtg tgtcaagagg cacctggccg
241 gaggtcaccc cctccttctg gaccctcagc agagttgagg cgcct
//
LOCUS AW269189 264 bp mRNA linear EST 03-JAN-2000
DEFINITION xs33e06.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2771458 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION AW269189
VERSION AW269189.1 GI:6656219
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 264)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from
Gibco.
FEATURES Location/Qualifiers
source 1..264
/db_xref="taxon:9606"
/clone="IMAGE:2771458"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 49 a 85 c 96 g 34 t
ORIGIN
1 attgattgcc gcgggctgcg ctctcatggg cgacccatgt ccccaagagc ggcactacat
61 agaggggttg gaggaagacg gtctgagaga agaggccgcg agagcccagg tgcacagaag
121 ccccggcccc acgggcctca cgcgccccag gaggtgtggg cagggcccgc cgcggaggga
181 ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc ccctccttct ggaccctcag
241 cagagttgag gcgcctgcac ccca
//
LOCUS BE048679 321 bp mRNA linear EST 08-JUN-2000
DEFINITION hr52c09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3132112 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);, mRNA
sequence.
ACCESSION BE048679
VERSION BE048679.1 GI:8365732
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 321)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL, send email to:
info@image.llnl.gov Seq primer: -40UP from Gibco.
FEATURES Location/Qualifiers
source 1..321
/db_xref="taxon:9606"
/clone="IMAGE:3132112"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 59 a 96 c 116 g 50 t
ORIGIN
1 gcttgtatca cctgctgttt attgattgcc gcgggctgcg ctctcatggg cgacccatgt
61 ccccaagagc ggcactacat agaggggttg gaggaagacg gtctgagaga agaggccgcg
121 agagcccagg tgcacagaag ccccggcccc acgggcctca cgcgccccag gaggtgtggg
181 cagggcccgc cgcggaggga ggctgcaggt gtgtcaggag gcacctggcc ggaggtcacc
241 ccctccttct ggaccctcag cagagttgag gcgcctgcac cccaggggag ccaggctgct
301 atctaaagga gtgtgaggtg c
//
LOCUS BE552434 474 bp mRNA linear EST 10-AUG-2000
DEFINITION hw26e12.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3184078 3'
similar to gb:U05569 ALPHA CRYSTALLIN A CHAIN (HUMAN);contains
TAR1.t1 TAR1 repetitive element ;, mRNA sequence.
ACCESSION BE552434
VERSION BE552434.1 GI:9794126
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 474)
AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap.
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished (1997)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R.
Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento
Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA
Sequencing by: Washington University Genome Sequencing Center
Clone distribution: NCI-CGAP clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL, send email to:
info@image.llnl.gov Seq primer: -40UP from Gibco High quality
sequence stop: 452.
FEATURES Location/Qualifiers
source 1..474
/db_xref="taxon:9606"
/clone="IMAGE:3184078"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NCI_CGAP_Kid11"
/note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with
a modified polylinker; Site_1: Not I; Site_2: Eco RI;
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was
prepared, and ss circles were made in vitro. Following
HAP purification, this DNA was used as tracer in a
subtractive hybridization reaction. The driver was
PCR-amplified cDNAs from a pool of 5,000 clones made from
the same library (cloneIDs 1322376-1323911,
1456007-1456775, and 1500552-1502855). Subtraction by
Bento Soares and M. Fatima Bonaldo. "
BASE COUNT 95 a 138 c 155 g 86 t
ORIGIN
1 tttttttttt tttgcttgta tcacctgctg tttattgatt gccgcgggct gcgctctcat
61 gggcgaccca tgtccccaag agcggcacta catagagggg ttggaggaag acggtctgag
121 agaagaggcc gcgagagccc aggtgcacag aagccccggc cccacgggcc tcacgcgccc
181 caggaggtgt gggcagggcc cgccgcggag ggaggctgca ggtgtgtcag gaggcacctg
241 gccggaggtc accccctcct tctggaccct cagcagagtt gaggcgcctg caccccaggg
301 gagccaggct gctatctaaa ggagtgtgag gtgcggtcac ttccgccact ggccggtgtc
361 ggggtctggg acaccctgag accctaagct ctcctggctg ctctctcaaa ccctcattcc
421 atgtgagaag gtggaaagga aaaaagggaa gcaaaggaag acagacaccc cgcc
481
//
LOCUS BF727324 698 bp mRNA linear EST 05-JAN-2001
DEFINITION by19h12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by19h12 5', mRNA sequence.
ACCESSION BF727324
VERSION BF727324.1 GI:12043235
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 698)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 19 row: h column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..698
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by19h12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 112 a 256 c 179 g 150 t 1 others
ORIGIN
1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac
61 gtgaccatcc agcacccctg gttcaagcgc accctggggc ccttctaccc cagccggctg
121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc
181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct
241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg
301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag
361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc
421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt
481 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg
541 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc
601 cctgcagccc tggcccatca tggggggagc accctgaggg cggngtgtct gtcttccttt
661 gcttcccttt tttcctttcc accttctcac atggaatg
//
LOCUS BF727028 629 bp mRNA linear EST 05-JAN-2001
DEFINITION by15g05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by15g05 5', mRNA sequence.
ACCESSION BF727028
VERSION BF727028.1 GI:12042939
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 629)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 15 row: g column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..629
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by15g05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 105 a 234 c 164 g 126 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg
541 agaagcccac ctcggctccc tcgtcctaag caggcattgc ctcggctggc tcccctgcag
601 ccctggccca tcatgggggg agcaccctg
//
LOCUS BF726180 600 bp mRNA linear EST 05-JAN-2001
DEFINITION by02b10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by02b10 5', mRNA sequence.
ACCESSION BF726180
VERSION BF726180.1 GI:12042091
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 600)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 02 row: b column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..600
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by02b10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 93 a 209 c 182 g 116 t
ORIGIN
1 gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg aggagaagcc
61 cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg cagccctggc
121 ccatcatggg gggagcaccc tgagggcggg gtgtctgtct tcctttgctt cccttttttc
181 ctttccacct tctcacatgg aatgagggtt tgagagagca gccaggagag cttagggtct
241 cagggtgtcc cagaccccga caccggccag tggcggaagt gaccgcacct cacactcctt
301 tagatagcag cctggctccc ctggggtgca ggcgcctcaa ctctgctgag ggtccagaag
361 gagggggtga cctccggcca ggtgcctcct gacacacctg cagcctccct ccgcggcggg
421 ccctgcccac acctcctggg gcgcgtgagg cccgtggggc cggggcttct gtgcacctgg
481 gctctcgcgg cctcttctct cagaccgtct tcctccaacc cctctatgta gtgccgctct
541 tggggacatg ggtcgcccat gagagcgcag cccgcggcaa tcaataaaca gcaggtgata
601
//
LOCUS BF726330 597 bp mRNA linear EST 05-JAN-2001
DEFINITION by05b01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by05b01 5', mRNA sequence.
ACCESSION BF726330
VERSION BF726330.1 GI:12042241
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 597)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 05 row: b column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..597
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by05b01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 97 a 216 c 161 g 123 t
ORIGIN
1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag
61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt
241 ctccccggag gacttcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca
301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct
361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgat
421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc
481 cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca ttgcctcggc
541 tggctcccct gcagccctgg cccatcatgg ggggagcacc ctgagggcgg ggtgtct
601
//
LOCUS BF726422 587 bp mRNA linear EST 05-JAN-2001
DEFINITION by06d05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by06d05 5', mRNA sequence.
ACCESSION BF726422
VERSION BF726422.1 GI:12042333
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 587)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 06 row: d column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..587
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by06d05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 98 a 217 c 154 g 117 t 1 others
ORIGIN
1 gctgaacatg gacgtgacca tccagcaccc ctggttcaag cgcaccctgg ggcccttcta
61 ccccagccgg ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc
121 cttcctgtcg tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga
181 ctccggcatc tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa
241 gcacttctcc ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg
301 aaagcacaac gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta
361 ccgcctgccg tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat
421 gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc
481 catccncgtg tcgcgggagg agaagcccac ctcggctccc tcgtcctaag caggcattgc
541 ctcggctggc tcccctgcag ccctggccca tcatgggggg agcaccc
//
LOCUS BF727295 592 bp mRNA linear EST 05-JAN-2001
DEFINITION by19e10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by19e10 5', mRNA sequence.
ACCESSION BF727295
VERSION BF727295.1 GI:12043206
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 592)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 19 row: e column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..592
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by19e10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 96 a 220 c 157 g 119 t
ORIGIN
1 gccgctcgcg atggatgtga ccatccagca cccctggttc aagcgcaccc tggggccctt
61 ctaccccagc cggctgttcg accagttttt cggcgagggc ctttttgagt atgacctgct
121 gcccttcctg tcgtccacca tcagccccta ctaccgccag tccctcttcc gcaccgtgct
181 ggactccggc atctctgagg ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt
241 gaagcacttc tccccggagg acctcaccgt gaaggtgcag gacgactttg tggagatcca
301 cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt tccaccgccg
361 ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt ctgccgatgg
421 catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc acgccgagcg
481 agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct aagcaggcat
541 tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc tg
601
//
LOCUS BF726236 576 bp mRNA linear EST 05-JAN-2001
DEFINITION by03f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by03f01 5', mRNA sequence.
ACCESSION BF726236
VERSION BF726236.1 GI:12042147
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 576)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 03 row: f column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..576
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by03f01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 100 a 214 c 147 g 115 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg
541 agaagcccac ctcggctccc tcgtcctaag caggca
//
LOCUS BF727002 577 bp mRNA linear EST 05-JAN-2001
DEFINITION by15c11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by15c11 5', mRNA sequence.
ACCESSION BF727002
VERSION BF727002.1 GI:12042913
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 577)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 15 row: c column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..577
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by15c11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 95 a 215 c 150 g 116 t 1 others
ORIGIN
1 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg
61 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc
121 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct
181 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg
241 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag
301 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc
361 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt
421 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg
481 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc
541 cctgcagccc tggcccatca tggggggagc accctga
//
LOCUS BF726253 577 bp mRNA linear EST 05-JAN-2001
DEFINITION by03h05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by03h05 5', mRNA sequence.
ACCESSION BF726253
VERSION BF726253.1 GI:12042164
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 577)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 03 row: h column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..577
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by03h05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 100 a 213 c 147 g 116 t 1 others
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc catccncgtg tcgcgggagg
541 agaagcccac ctcggctccc tcgtcctaag caggcat
//
LOCUS BF726679 558 bp mRNA linear EST 05-JAN-2001
DEFINITION by10d02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by10d02 5', mRNA sequence.
ACCESSION BF726679
VERSION BF726679.1 GI:12042590
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 558)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 10 row: d column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..558
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by10d02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 92 a 209 c 142 g 114 t 1 others
ORIGIN
1 catccagcac ccctggttca agcgcaccct gnggcccttc taccccagcc ggctgttcga
61 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat
121 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt
181 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga
241 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca
301 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt
361 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc
421 caagatccag actggcctgg atgcaaccca cgccgagcga gccatccccg tgtcgcggga
481 ggagaagccc acctcggctc cctcgtccta agcaggcatt gcctcggctg gctcccctgc
541 agccctggcc catcatgg
//
LOCUS BF726363 544 bp mRNA linear EST 05-JAN-2001
DEFINITION by05e10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by05e10 5', mRNA sequence.
ACCESSION BF726363
VERSION BF726363.1 GI:12042274
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 544)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 05 row: e column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..544
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by05e10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 93 a 187 c 152 g 112 t
ORIGIN
1 gcaccgtgct ggactccggc atctctgagg ttcgatccga ccgggacaag ttcgtcatct
61 tcctcgatgt gaagcacttc tccccggagg acctcaccgt gaaggtgcag gacgactttg
121 tggagatcca cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt
181 tccaccgccg ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt
241 ctgccgatgg catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc
301 acgccgagcg agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct
361 aagcaggcat tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc
421 tgagggcggg gtgtctgtct tcctttgctt cccttttttc ctttccacct tctcacatgg
481 aatgagggtt tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga
541 cacc
//
LOCUS BF726381 536 bp mRNA linear EST 05-JAN-2001
DEFINITION by05g11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by05g11 5', mRNA sequence.
ACCESSION BF726381
VERSION BF726381.1 GI:12042292
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 536)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 05 row: g column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..536
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by05g11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 197 c 137 g 111 t
ORIGIN
1 gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg atgtgaccat
61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca
121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag
181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg
241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct
301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga
361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga
421 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa
481 gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcggg
541
//
LOCUS BF726880 527 bp mRNA linear EST 05-JAN-2001
DEFINITION by13d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by13d10 5', mRNA sequence.
ACCESSION BF726880
VERSION BF726880.1 GI:12042791
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 527)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 13 row: d column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..527
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by13d10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 194 c 135 g 107 t
ORIGIN
1 gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg acgtgaccat ccagcacccc
61 tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca gtttttcggc
121 gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag cccctactac
181 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg
241 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag
301 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc
361 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc
421 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact
481 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcggga
//
LOCUS BF726441 524 bp mRNA linear EST 05-JAN-2001
DEFINITION by06f04.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by06f04 5', mRNA sequence.
ACCESSION BF726441
VERSION BF726441.1 GI:12042352
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 524)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 06 row: f column: 04 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..524
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by06f04"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 194 c 131 g 108 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc catc
//
LOCUS BF726358 532 bp mRNA linear EST 05-JAN-2001
DEFINITION by05d12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by05d12 5', mRNA sequence.
ACCESSION BF726358
VERSION BF726358.1 GI:12042269
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 532)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 05 row: d column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..532
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by05d12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 194 c 136 g 111 t
ORIGIN
1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag
61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt
241 ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca
301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct
361 gccgtccaac gtggaccagt tggccctctc ttgctccctg tctgccgatg gcatgctgac
421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc
481 cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca tt
541
//
LOCUS BF726890 523 bp mRNA linear EST 05-JAN-2001
DEFINITION by13f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by13f01 5', mRNA sequence.
ACCESSION BF726890
VERSION BF726890.1 GI:12042801
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 523)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 13 row: f column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..523
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by13f01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 84 a 190 c 141 g 108 t
ORIGIN
1 cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca gcccctacta
61 ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc gatccgaccg
121 ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa
181 ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg acgaccacgg
241 ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg accagtcggc
301 cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca agatccagac
361 tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg agaagcccac
421 ctcggctccc tcgtcctaag caggcattgc ctcggctggc tcccctgcag ccctggccca
481 tcatgggggg agcaccctga gggcggggtg tctgtcttcc ttt
//
LOCUS BF726860 519 bp mRNA linear EST 05-JAN-2001
DEFINITION by13b07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by13b07 5', mRNA sequence.
ACCESSION BF726860
VERSION BF726860.1 GI:12042771
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 519)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 13 row: b column: 07 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..519
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by13b07"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 90 a 191 c 131 g 107 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgag
//
LOCUS BF726399 536 bp mRNA linear EST 05-JAN-2001
DEFINITION by06a09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by06a09 5', mRNA sequence.
ACCESSION BF726399
VERSION BF726399.1 GI:12042310
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 536)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 06 row: a column: 09 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..536
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by06a09"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 90 a 198 c 138 g 109 t 1 others
ORIGIN
1 ggccgctcgc catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct
61 tctaccccag ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc
121 tgcccttcct gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc
181 tggactccgg catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg
241 tgaagcactt ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc
301 acggaaagca caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgnc
361 gctaccgcct gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg
421 gcatgctgac cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc
481 gagccatccc cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagca
541
//
LOCUS BF726109 522 bp mRNA linear EST 05-JAN-2001
DEFINITION by01b12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by01b12 5', mRNA sequence.
ACCESSION BF726109
VERSION BF726109.1 GI:12042020
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 522)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 01 row: b column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..522
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by01b12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 192 c 131 g 108 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca
61 tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc ca
//
LOCUS BF726854 518 bp mRNA linear EST 05-JAN-2001
DEFINITION by13a10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by13a10 5', mRNA sequence.
ACCESSION BF726854
VERSION BF726854.1 GI:12042765
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 518)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 13 row: a column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..518
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by13a10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 88 a 192 c 132 g 106 t
ORIGIN
1 catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc ggctgttcga
61 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat
121 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt
181 tcgatccgac cgggacaagt tcgtcatctt tctcgatgtg aagcacttct ccccggagga
241 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca
301 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt
361 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc
421 caagatccag actggcctgg atgccaccca cgccgagcga gccatccccg tgtcgcggga
481 ggagaagccc acctcggctc cctcgtccta agcaggca
//
LOCUS BF727140 519 bp mRNA linear EST 05-JAN-2001
DEFINITION by17d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by17d03 5', mRNA sequence.
ACCESSION BF727140
VERSION BF727140.1 GI:12043051
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 519)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 17 row: d column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..519
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by17d03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 90 a 191 c 131 g 107 t
ORIGIN
1 gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg atgtgaccat
61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca
121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag
181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg
241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct
301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga
361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga
421 ccagtcggcc ctctctcgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa
481 gatccagact ggcctggatg ccacccacgc cgagcgagc
//
LOCUS BF727010 516 bp mRNA linear EST 05-JAN-2001
DEFINITION by15d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by15d10 5', mRNA sequence.
ACCESSION BF727010
VERSION BF727010.1 GI:12042921
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 516)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 15 row: d column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..516
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by15d10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 90 a 190 c 128 g 107 t 1 others
ORIGIN
1 ctcctgccag cctccaggtc cccgtggtac caaagctgaa catggatgtg accatccagc
61 acccctggtt caagcgcacc ctgnggccct tctaccccag ccggctgttc gaccagtttt
121 tcggcgaggg cctttttgag tatgacctgc tgcccttcct gtcgtccacc atcagcccct
181 actaccgcca gtccctcttc cgcaccgtgc tggactccgg catctctgag gttcgatccg
241 accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag gacctcaccg
301 tgaaggtgca ggacgacttt gtggagatcc acggaaagca caacgagcgc caggacgacc
361 acggctacat ttcccgtgag ttccaccgcc gctaccgcct gccgtccaac gtggaccagt
421 cggccctctc ttgctccctg tctgccgatg gcatgctgac cttctgtggc cccaagatcc
481 agactggcct ggatgccacc cacgccgagc gagcca
//
LOCUS BF726595 522 bp mRNA linear EST 05-JAN-2001
DEFINITION by09a09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by09a09 5', mRNA sequence.
ACCESSION BF726595
VERSION BF726595.1 GI:12042506
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 522)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 09 row: a column: 09 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..522
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by09a09"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 192 c 129 g 109 t 1 others
ORIGIN
1 cgctgactcc tgccagcctc caggtcctcc tggtaccaaa gctgaacatg gatgtgacca
61 tccagcaccc ctggttcaag cgcaccctgn ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg
421 accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc tgtggcccca
481 agatccagac tggcctggat gccacccacg ccgagcgagc ca
//
LOCUS BF726438 523 bp mRNA linear EST 05-JAN-2001
DEFINITION by06f01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by06f01 5', mRNA sequence.
ACCESSION BF726438
VERSION BF726438.1 GI:12042349
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 523)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 06 row: f column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..523
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by06f01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 89 a 192 c 139 g 103 t
ORIGIN
1 cgcgagcggg agcggccatg gacgtgacca tccagcaccc ctggttcaag cgcaccctgg
61 ggcccttcta ccccagccgg ctgttcgacc agtttttcgg cgagggcctt tttgagtatg
121 acctgctgcc cttcctgtcg tccaccatca gcccctacta ccgccagtcc ctcttccgca
181 ccgtgctgga ctccggcatc tctgaggttc gatccgaccg ggacaagttc gtcatcttcc
241 tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg
301 agatccacgg aaagcacaac gagcgccagg acgaccacgg ctacatttcc cgtgagttcc
361 accgccgcta ccgcctgccg tccaacgtgg accagtcggc cctctcttgc tccctgtctg
421 ccgatggcat gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg
481 ccgagcgagc catccccgtg tcgcgggagg agaagcccac ctc
//
LOCUS BF726928 521 bp mRNA linear EST 05-JAN-2001
DEFINITION by14b12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by14b12 5', mRNA sequence.
ACCESSION BF726928
VERSION BF726928.1 GI:12042839
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 521)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 14 row: b column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..521
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by14b12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 91 a 193 c 130 g 107 t
ORIGIN
1 ccccgctgac tcctgccagc ctccaggtcc ccgtggtacc aaagctgaac atggatgtga
61 ccatccagca cccctggttc aagcgcaccc tggggccctt ctaccccagc cggctgttcg
121 accagttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat
181 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt
241 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccagagga
301 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca
361 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt
421 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc
481 caagatccag actggcctgg atgccaccca cgccgagcga g
//
LOCUS BF727221 506 bp mRNA linear EST 05-JAN-2001
DEFINITION by18e03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18e03 5', mRNA sequence.
ACCESSION BF727221
VERSION BF727221.1 GI:12043132
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 506)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: e column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..506
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18e03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 88 a 183 c 128 g 106 t 1 others
ORIGIN
1 gtggtaccaa agctgaacat ggatgtgacc atccagcacc cctggttcaa gcgcaccctg
61 nggcccttct accccagccg gctgttcgac cagtttttcg gcgagggcct ttttgagtat
121 gacctgctgc ccttcctgtc gtccaccatc agcccctact accgccagtc cctcttccgc
181 accgtgctgg actccggcat ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc
241 ctcgatgtga agcacttctc cccggaggac ctcaccgtga aggtgcagga cgactttgtg
301 gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc ccgtgagttc
361 caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct
421 gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac
481 gccgagcgag ccatccccgt gtcgcg
//
LOCUS BF727170 499 bp mRNA linear EST 05-JAN-2001
DEFINITION by17g05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by17g05 5', mRNA sequence.
ACCESSION BF727170
VERSION BF727170.1 GI:12043081
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 499)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 17 row: g column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..499
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by17g05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 88 a 172 c 140 g 99 t
ORIGIN
1 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga
61 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga
121 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa
181 gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga
241 gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc
301 cctggcccat catgggggga gcaccctgag ggcggggtgt ctgtcttcct ttgcttccct
361 tttttccttt ccaccttctc acatggaatg agggtttgag agagcagcca ggagagctta
421 gggtctcagg gtgtcccaga ccccgacacc ggccagtggc ggaagtgacc gcacctcaca
481 ctcctttaga tagcagcct
//
LOCUS BF727239 500 bp mRNA linear EST 05-JAN-2001
DEFINITION by18g02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18g02 5', mRNA sequence.
ACCESSION BF727239
VERSION BF727239.1 GI:12043150
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 500)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: g column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..500
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18g02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 180 c 131 g 107 t 1 others
ORIGIN
1 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg
61 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cagaggacct caccgtgaag
121 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc
181 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc
241 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact
301 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc
361 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat
421 catgggggga gcaccctgag ggcggngtgt ctgtcttcct ttgcttccct tttttccttt
481 ccaccttctc acatggaatg
//
LOCUS BF726856 492 bp mRNA linear EST 05-JAN-2001
DEFINITION by13b01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by13b01 5', mRNA sequence.
ACCESSION BF726856
VERSION BF726856.1 GI:12042767
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 492)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 13 row: b column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..492
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by13b01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 78 a 174 c 140 g 100 t
ORIGIN
1 acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc
61 cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct
121 tctgtggccc caagatccag actggcctgg atgccaccca cgccgagcga gccatccccg
181 tgtcgcggga ggagaagccc acctcggctc cctcgtccta agcaggcatt gcctcggctg
241 gctcccctgc agccctggcc catcatgggg ggagcaccct gagggcgggg tgtctgtctt
301 cctttgcttc ccttttttcc tttccacctt ctcacatgga atgagggttt gagagagcag
361 ccaggagagc ttagggtctc agggtgtccc agaccccgac accggccagt ggcggaagtg
421 accgcacctc acactccttt agatagcagc ctggctcccc tggggtgcag gcgcctcaac
481 tctgctgagg gt
//
LOCUS BF727205 503 bp mRNA linear EST 05-JAN-2001
DEFINITION by18b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18b11 5', mRNA sequence.
ACCESSION BF727205
VERSION BF727205.1 GI:12043116
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 503)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: b column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..503
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18b11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 89 a 183 c 128 g 103 t
ORIGIN
1 catggatgtg accatccagc acccctggtt caagcgcacc ctgaggccct tctaccccag
61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt
241 ctccccagag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca
301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct
361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac
421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc
481 cgtgtcgcgg gaggagaagc cca
//
LOCUS BF727264 485 bp mRNA linear EST 05-JAN-2001
DEFINITION by19b05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by19b05 5', mRNA sequence.
ACCESSION BF727264
VERSION BF727264.1 GI:12043175
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 485)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 19 row: b column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..485
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by19b05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 180 c 122 g 101 t 1 others
ORIGIN
1 gacgtgacca tccagcaccc ctggttcaag cgcaccctgn ggcccttcta ccccagccgg
61 ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg
121 tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc
181 tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc
241 ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac
301 gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg
361 tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc
421 tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg
481 tcgcg
//
LOCUS BF727252 477 bp mRNA linear EST 05-JAN-2001
DEFINITION by18h10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18h10 5', mRNA sequence.
ACCESSION BF727252
VERSION BF727252.1 GI:12043163
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 477)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: h column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..477
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18h10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 176 c 118 g 101 t 1 others
ORIGIN
1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac
61 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg
121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc
181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct
241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg
301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag
361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc
421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttc
481
//
LOCUS BF727348 480 bp mRNA linear EST 05-JAN-2001
DEFINITION by20c08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by20c08 5', mRNA sequence.
ACCESSION BF727348
VERSION BF727348.1 GI:12043259
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 480)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 20 row: c column: 08 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..480
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by20c08"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 80 a 179 c 120 g 101 t
ORIGIN
1 ggccgctcgc cccgctgact cctgccagcc tccaggtccc cgtggtacca aagctgaaca
61 tggacgtgac catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc
121 ggctgttcga ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt
181 cgtccaccat cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca
241 tctctgaggt tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct
301 ccccggagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca
361 acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc
421 cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct
481
//
LOCUS BF727230 486 bp mRNA linear EST 05-JAN-2001
DEFINITION by18e12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18e12 5', mRNA sequence.
ACCESSION BF727230
VERSION BF727230.1 GI:12043141
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 486)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: e column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..486
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18e12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 83 a 169 c 142 g 92 t
ORIGIN
1 gcgagcggcc ccccagagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac
61 ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccgccgc
121 taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc tgccgatggc
181 atgctgacct tctgtggccc caagatccag actggcctgg atgccaccca cgccgagcga
241 gccatccccg tgtcgcggga ggagaagccc acctcggctc cctcgtccta agcaggcatt
301 gcctcggctg gctcccctgc agccctggcc catcatgggg ggagcaccct gagggcgggg
361 tgtctgtctt cctttgcttc ccttttttcc tttccacctt ctcacatgga atgagggttt
421 gagagagcag ccaggagagc ttagggtctc agggtgtccc agaccccgac accggccagt
481 ggcgga
//
LOCUS BF727402 479 bp mRNA linear EST 05-JAN-2001
DEFINITION by20h11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by20h11 5', mRNA sequence.
ACCESSION BF727402
VERSION BF727402.1 GI:12043313
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 479)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 20 row: h column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..479
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by20h11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 82 a 179 c 118 g 100 t
ORIGIN
1 gaccatccag cacccctggt tcaagcgcac cctgaggccc ttctacccca gccggctgtt
61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac
121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga
181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccaga
241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg
301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa
361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg
421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgc
481
//
LOCUS BF726931 473 bp mRNA linear EST 05-JAN-2001
DEFINITION by14c03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by14c03 5', mRNA sequence.
ACCESSION BF726931
VERSION BF726931.1 GI:12042842
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 473)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 14 row: c column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..473
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by14c03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 173 c 120 g 99 t
ORIGIN
1 ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct accccagccg
61 gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc ccttcctgtc
121 gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg actccggcat
181 ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc ctcgatgtga agcacttctc
241 cccggaggac ctcaccgtga aggtgcagga cgactttgtg gagatccacg gaaagcacaa
301 cgagcgccag gacgaccacg gctacatttc ccgtgagttc caccgccgct accgcctgcc
361 gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct gccgatggca tgctgacctt
421 ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag cca
481
//
LOCUS BF726645 489 bp mRNA linear EST 05-JAN-2001
DEFINITION by09g09.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by09g09 5', mRNA sequence.
ACCESSION BF726645
VERSION BF726645.1 GI:12042556
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 489)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 09 row: g column: 09 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..489
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by09g09"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 83 a 179 c 122 g 104 t 1 others
ORIGIN
1 ggccgctcga gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct
61 gaacatggat gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc
121 cagccggctg ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt
181 cctgtcgtcc accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc
241 cggcatctct gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca
301 cttctcccca gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa
361 gcacaacgag cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg
421 cctgccgtcc aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct
481 gaccttctg
//
LOCUS BF727399 474 bp mRNA linear EST 05-JAN-2001
DEFINITION by20h07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by20h07 5', mRNA sequence.
ACCESSION BF727399
VERSION BF727399.1 GI:12043310
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 474)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 20 row: h column: 07 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..474
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by20h07"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 173 c 119 g 100 t 1 others
ORIGIN
1 catggatgtg accatccagc acccctggtt caagcgcacc ctgnggccct tctaccccag
61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
181 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt
241 ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca
301 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct
361 gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac
421 cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc gagc
481
//
LOCUS BF726920 471 bp mRNA linear EST 05-JAN-2001
DEFINITION by14b03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by14b03 5', mRNA sequence.
ACCESSION BF726920
VERSION BF726920.1 GI:12042831
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 471)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 14 row: b column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..471
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by14b03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 80 a 171 c 117 g 103 t
ORIGIN
1 gctgactcct gccagcctcc aggtcctcgt ggtaccaaag ctgaacatgg acgtgaccat
61 ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc tgttcgacca
121 gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt ccaccatcag
181 cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg
241 atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct
301 caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga
361 cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga
421 ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct g
481
//
LOCUS BF727220 466 bp mRNA linear EST 05-JAN-2001
DEFINITION by18d12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18d12 5', mRNA sequence.
ACCESSION BF727220
VERSION BF727220.1 GI:12043131
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 466)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: d column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..466
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18d12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 80 a 174 c 126 g 86 t
ORIGIN
1 gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg actccggcat
61 ctctgaggtt cgatccgacc gggacaagtt cgtcatcttc ctcgatgtga agcacttctc
121 cccggaggac ctcaccgtga aggtgcagga cgactttgtg gagatccacg gaaagcacaa
181 cgagcgccag gacgaccacg gctacatttc ccgtgagttc caccgccgct accgcctgcc
241 gtccaacgtg gaccagtcgg ccctctcttg ctccctgtct gccgatggca tgctgacctt
301 ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag ccatccccgt
361 gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa gcaggcattg cctcggctgg
421 ctcccctgca gccctggccc atcatggggg gagcaccctg agggcg
//
LOCUS BF726480 462 bp mRNA linear EST 05-JAN-2001
DEFINITION by07b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by07b11 5', mRNA sequence.
ACCESSION BF726480
VERSION BF726480.1 GI:12042391
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 462)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 07 row: b column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..462
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by07b11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 79 a 159 c 127 g 97 t
ORIGIN
1 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag
61 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc
121 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc
181 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact
241 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc
301 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat
361 catgggggga gcaccctgag ggcggggtgt ctgtcttcct ttgcttccct tttttccttt
421 ccaccttctc acatggaatg agggtttgag agagcagcca gg
//
LOCUS BF727255 472 bp mRNA linear EST 05-JAN-2001
DEFINITION by19a03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by19a03 5', mRNA sequence.
ACCESSION BF727255
VERSION BF727255.1 GI:12043166
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 472)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 19 row: a column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..472
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by19a03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 80 a 173 c 117 g 101 t 1 others
ORIGIN
1 ggccccgctg actcctgcca gcctccaggt ccccgtggta ccaaagctga acatggatgt
61 gaccatccag cacccctggt tcaagcgcac cctgnggccc ttctacccca gccggctgtt
121 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgttgtccac
181 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga
241 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga
301 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg
361 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa
421 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga cc
481
//
LOCUS BF726623 466 bp mRNA linear EST 05-JAN-2001
DEFINITION by09d10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by09d10 5', mRNA sequence.
ACCESSION BF726623
VERSION BF726623.1 GI:12042534
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 466)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 09 row: d column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..466
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by09d10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 74 a 158 c 137 g 96 t 1 others
ORIGIN
1 caccctgagg gcggngtgtc tgtcttcctt tgcttccctt ttttcctttc caccttctca
61 catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg tgtcccagac
121 cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat agcagcctgg
181 ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg ggtgacctcc
241 ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gcgggccctg cccacacctc
301 ctggggcgcg tgaggcccgt ggggccgggg cttctgtgca cctgggctct cgcggcctct
361 tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg acatgggtcg
421 cccatgagag cacagcccgc ggcaatcaat aaacagcagg tgatac
//
LOCUS BF727464 460 bp mRNA linear EST 05-JAN-2001
DEFINITION by21h03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by21h03 5', mRNA sequence.
ACCESSION BF727464
VERSION BF727464.1 GI:12043375
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 460)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 21 row: h column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..460
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by21h03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 79 a 170 c 126 g 85 t
ORIGIN
1 tcagccccta ctaccgccag tccctcttcc gcaccgtgct ggactccggc atctctgagg
61 ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccagagg
121 acctcaccgt gaaggtgcag gacgactttg tggagatcca cggaaagcac aacgagcgcc
181 aggacgacca cggctacatt tcccgtgagt tccaccgccg ctaccgcctg ccgtccaacg
241 tggaccagtc ggccctctct tgctccctgt ctgccgatgg catgctgacc ttctgtggcc
301 ccaagatcca gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg
361 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg
421 cagccctggc ccatcatggg gggagcaccc tgagggcggg
//
LOCUS BF726725 457 bp mRNA linear EST 05-JAN-2001
DEFINITION by11a06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by11a06 5', mRNA sequence.
ACCESSION BF726725
VERSION BF726725.1 GI:12042636
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 457)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 11 row: a column: 06 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..457
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by11a06"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 79 a 166 c 114 g 98 t
ORIGIN
1 tgccagcctc caggtccccg tggtaccaaa gctgaacatg gatgtgacca tccagcaccc
61 ctggttcaag cgcaccctgg ggcccttcta ccccagccgg ctgttcgacc agtttttcgg
121 cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca gcccctacta
181 ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc gatccgaccg
241 ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc tcaccgtgaa
301 ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg acgaccacgg
361 ctacatttcc cgtgagttcc accgccgcta ccgcctgccg tccaacgtgg accagtcggc
421 cctctcttgc tccctgtctg ccgatggcat gctgacc
//
LOCUS BF726315 466 bp mRNA linear EST 05-JAN-2001
DEFINITION by04g12.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by04g12 5', mRNA sequence.
ACCESSION BF726315
VERSION BF726315.1 GI:12042226
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 466)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 04 row: g column: 12 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..466
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by04g12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 90 a 153 c 131 g 92 t
ORIGIN
1 gtcttccttt gcttcccttt tttcctttcc accttctcac atggaatgag ggtttgagag
61 agcagccagg agagcttagg gtctcagggt gtcccagacc ccgacaccgg ccagtggcgg
121 aagtgaccgc acctcacact cctttagata gcagcctggc tcccctgggg tgcaggcgcc
181 tcaactctgc tgagggtcca gaaggagggg gtgacctccg gccaggtgcc tcctgacaca
241 cctgcagcct ccctccgcgg cgggccctgc ccacacctcc tggggcgcgt gaggcccgtg
301 gggccggggc ttctgtgcac ctgggctctc gcggcctctt ctctcagacc gtcttcctcc
361 aacccctcta tgtagtgccg ctcttgggga catgggtcgc ccatgagagc acagcccgcg
421 gcaatcaata aacagcaggt gatacaagca aaaaaaaaaa aaaaag
//
LOCUS BF726515 451 bp mRNA linear EST 05-JAN-2001
DEFINITION by07g02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by07g02 5', mRNA sequence.
ACCESSION BF726515
VERSION BF726515.1 GI:12042426
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 451)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 07 row: g column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..451
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by07g02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 77 a 162 c 112 g 100 t
ORIGIN
1 cgtggtacca aagctgaaca tggatgtgac catccagcac ccctggttca agcgcaccct
61 gtggcccttc taccccagcc ggctgttcga ccagtttttc ggcgagggcc tttttgagta
121 tgacctgctg cccttcctgt cgtccaccat cagcccctac taccgccagt ccctcttccg
181 caccgtgctg gactccggca tctctgaggt tcgatccgac cgggacaagt tcgtcatctt
241 cctcgatgtg aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt
301 ggagatccac ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt
361 ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc
421 tgccgatggc atgctgacct tctgtggccc c
//
LOCUS BF727355 438 bp mRNA linear EST 05-JAN-2001
DEFINITION by20d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by20d03 5', mRNA sequence.
ACCESSION BF727355
VERSION BF727355.1 GI:12043266
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 438)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 20 row: d column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..438
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by20d03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 74 a 162 c 120 g 82 t
ORIGIN
1 cgccagtccc tcttccgcac cgtgctggac tccggcatct ctgaggttcg atccgaccgg
61 gacaagttcg tcatcttcct cgatgtgaag cacttctccc cggaggacct caccgtgaag
121 gtgcaggacg actttgtgga gatccacgga aagcacaacg agcgccagga cgaccacggc
181 tacatttccc gtgagttcca ccgccgctac cgcctgccgt ccaacgtgga ccagtcggcc
241 ctctcttgct ccctgtctgc cgatggcatg ctgaccttct gtggccccaa gatccagact
301 ggcctggatg ccacccacgc cgagcgagcc atccccgtgt cgcgggagga gaagcccacc
361 tcggctccct cgtcctaagc aggcattgcc tcggctggct cccctgcagc cctggcccat
421 catgggggga gcaccctg
//
LOCUS BF727245 432 bp mRNA linear EST 05-JAN-2001
DEFINITION by18h02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by18h02 5', mRNA sequence.
ACCESSION BF727245
VERSION BF727245.1 GI:12043156
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 432)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 18 row: h column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..432
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by18h02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 74 a 146 c 128 g 84 t
ORIGIN
1 cttttttcct ttccaccttc tcacatggaa tgagggtttg agagagcagc caggagagct
61 tagggtctca gggtgtccca gaccccgaca ccggccagtg gcggaagtga ccgcacctca
121 cactccttta gatagcagcc tggctcccct ggggtgcagg cgcctcaact ctgctgaggg
181 tccagaagga gggggtgacc tccggccagg tgcctcctga cacacctgca gcctccctcc
241 gcggcgggcc ctgcccacac ctcctggggc gcgtgaggcc cgtggggccg gggcttctgt
301 gcacctgggc tctcgcggcc tcttctctca gaccgtcttc ctccaacccc tctatgtagt
361 gccgctcttg gggacatggg tcgcccatga gagcacagcc cgcggcaatc aataaacagc
421 aggtgataca ag
//
LOCUS BF726487 428 bp mRNA linear EST 05-JAN-2001
DEFINITION by07c08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by07c08 5', mRNA sequence.
ACCESSION BF726487
VERSION BF726487.1 GI:12042398
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 428)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 07 row: c column: 08 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..428
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by07c08"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 65 a 151 c 123 g 89 t
ORIGIN
1 cgctaccgcc tgccgtccaa cgtggaccag tcggccctct cttgctccct gtctgccgat
61 ggcatgctga ccttctgtgg ccccaagatc cagactggcc tggatgccac ccacgccgag
121 cgagccatcc ccgtgtcgcg ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc
181 attgcctcgg ctggctcccc tgcagccctg gcccatcatg gggggagcac cctgagggcg
241 gggtgtctgt cttcctttgc ttcccttttt tcctttccac cttctcacat ggaatgaggg
301 tttgagagag cagccaggag agcttagggt ctcagggtgt cccagacccc gacaccggcc
361 agtggcggaa gtgaccgcac cttacactcc tttagatagc agcctggctc ccctggggtg
421 caggcgcc
//
LOCUS BF726678 412 bp mRNA linear EST 05-JAN-2001
DEFINITION by10d01.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by10d01 5', mRNA sequence.
ACCESSION BF726678
VERSION BF726678.1 GI:12042589
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 412)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 10 row: d column: 01 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..412
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by10d01"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 55 a 143 c 128 g 86 t
ORIGIN
1 tgcctcggct ggctcccctg catccctggc ccatcatggg gggagcaccc tgagggcggg
61 gtgtctgtct tcctttgctt cccttttttc ctttccacct tctcacatgg aatgagggtt
121 tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga caccggccag
181 tggcggaagt gaccgcacct cacactcctt tagatagcag cctggctccc ctggggtgca
241 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct
301 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg
361 cccgtggggc cggggcttct gtgcacctgg gctctcgcgg cctcttctct ca
421
//
LOCUS BF726250 407 bp mRNA linear EST 05-JAN-2001
DEFINITION by03h02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by03h02 5', mRNA sequence.
ACCESSION BF726250
VERSION BF726250.1 GI:12042161
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 407)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 03 row: h column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..407
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by03h02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 64 a 143 c 114 g 86 t
ORIGIN
1 ccgctaccgc ctgccgtcca acgtggacca gtcggccctc tcttgctccc tgtctgccga
61 tggcatgctg accttctgtg gccccaagat ccagactggc ctggatgcca cccacgccga
121 gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg gctccctcgt cctaagcagg
181 cattgcctcg gctggctccc ctgcagccct ggcccatcat ggggggagca ccctgagggc
241 ggggtgtctg tcttcctttg gtttcccttt tttcctttcc accttctcac atggaatgag
301 ggtttgagag agcagccagg agagcttagg gtctcagggt gtcccagacc ccgacaccgg
361 ccagtggcgg aagtgaccgc acctcacact cctttagata gcagcct
//
LOCUS BF726707 390 bp mRNA linear EST 05-JAN-2001
DEFINITION by10g06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by10g06 5', mRNA sequence.
ACCESSION BF726707
VERSION BF726707.1 GI:12042618
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 390)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 10 row: g column: 06 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..390
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by10g06"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 71 a 139 c 98 g 82 t
ORIGIN
1 ggccccgctg actcctgcca gcctccaggt ccccgtggta ccaaagctga acatggatgt
61 gaccatccag cacccctggt tcaagcgcac cctggggccc ttctacccca gccggctgtt
121 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac
181 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga
241 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga
301 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg
361 ccaggacgac cacggctaca tttcccgtga
//
LOCUS BF726516 388 bp mRNA linear EST 05-JAN-2001
DEFINITION by07g03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by07g03 5', mRNA sequence.
ACCESSION BF726516
VERSION BF726516.1 GI:12042427
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 388)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 07 row: g column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..388
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by07g03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 71 a 137 c 96 g 84 t
ORIGIN
1 cgctgactcc tgccagcctc caggtccccg tggtaccaaa gctgaacatg gacgtgacca
61 tccagcaccc ctggttcaag cgcaccctgt ggcccttcta ccccagccgg ctgttcgacc
121 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
181 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
241 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
301 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
361 acgaccacgg ctacatttcc cgtgagtt
//
LOCUS BF726527 402 bp mRNA linear EST 05-JAN-2001
DEFINITION by07h08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by07h08 5', mRNA sequence.
ACCESSION BF726527
VERSION BF726527.1 GI:12042438
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 402)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 07 row: h column: 08 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..402
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by07h08"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 68 a 141 c 102 g 91 t
ORIGIN
1 ccttctaccc cagccggctg ttcgaccagt ttttcggcga gggccttttt gagtatgacc
61 tgctgccctt cctgtcgtcc accatcagcc cctactaccg ccagtccctc ttccgcaccg
121 tgctggactc cggcatctct gaggttcgat ccgaccggga caagtttgtc atcttcctcg
181 atgtgaagca cttctccccg gaggacctca ccgtgaaggt gcaggacgac tttgtggaga
241 tccacggaaa gcacaacgag cgccaggacg accacggcta catttcccgt gagttccacc
301 gacggtaccg cctgccgtcc aacgtggacc agtcggccct ctcttgctcc ctgtctgccg
361 atgggatgct gaccttctgt gggcccaaga tccagactgg cc
//
LOCUS BF727001 406 bp mRNA linear EST 05-JAN-2001
DEFINITION by15c10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by15c10 5', mRNA sequence.
ACCESSION BF727001
VERSION BF727001.1 GI:12042912
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 406)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 15 row: c column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..406
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by15c10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 72 a 147 c 103 g 82 t 2 others
ORIGIN
1 gcgagcggcc ggccgctcgc ctcctgccag cctccaggtc cccgtggtac caaagctgaa
61 catggacgtg accatccagc acccctggtt caagcgcacc ctgnggccct tctaccccag
121 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
181 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
241 catctctgag gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt
301 ctccccggag gacctcaccg tgaaggtgca ggacgactnt gtggagatcc acggaaagca
361 caacgagcgc caggacgacc acggctacat ttcccgtgag ttccac
//
LOCUS BF726268 394 bp mRNA linear EST 05-JAN-2001
DEFINITION by04b05.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by04b05 5', mRNA sequence.
ACCESSION BF726268
VERSION BF726268.1 GI:12042179
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 394)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 04 row: b column: 05 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..394
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by04b05"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 72 a 138 c 101 g 83 t
ORIGIN
1 gcgagcggcc gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggatg
61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt
121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca
181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg
241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg
301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc
361 gccaggacga ccacggctac atttcccgtg agtt
//
LOCUS BF726942 369 bp mRNA linear EST 05-JAN-2001
DEFINITION by14d08.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by14d08 5', mRNA sequence.
ACCESSION BF726942
VERSION BF726942.1 GI:12042853
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 369)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 14 row: d column: 08 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..369
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by14d08"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 60 a 127 c 107 g 75 t
ORIGIN
1 tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac gccgagcgag
61 ccatccccgt gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa gcaggcattg
121 cctcggctgg ctcccctgca gccctggccc atcatggggg gagcaccctg agggcggggt
181 gtctgtcttc ctttgcttcc cttttttcct ttccaccttc tcacatggaa tgagggtttg
241 agagagcagc caggagagct tagggtctca gggtgtccca gaccccgaca ccggccagtg
301 gcggaagtga ccgcacctca cactccttta gatagcagcc tggctcccct ggggtgcagg
361 cgcctcaac
//
LOCUS BF726599 338 bp mRNA linear EST 05-JAN-2001
DEFINITION by09b02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by09b02 5', mRNA sequence.
ACCESSION BF726599
VERSION BF726599.1 GI:12042510
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 338)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 09 row: b column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..338
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by09b02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 61 a 118 c 83 g 74 t 2 others
ORIGIN
1 gtgaccatcc agcacccctg gttcaagcgc accctgnggc ccttctaccc cagccggctg
61 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc
121 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct
181 gaggttcgat ccgaccggga caagttcgtc atctttctcg atgtgaagca cttctccccg
241 gaggacctca ccgtgaaggt gcaggacgac tntgtggaga tccacggaaa gcacaacgag
301 cgccaggacg accacggcta catttcccgt gagttcca
//
LOCUS BF726132 331 bp mRNA linear EST 05-JAN-2001
DEFINITION by01e10.x1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by01e10 3', mRNA sequence.
ACCESSION BF726132
VERSION BF726132.1 GI:12042043
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 331)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 01 row: e column: 10 Seq primer: -21M13 forward primer
(ABI).
FEATURES Location/Qualifiers
source 1..331
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by01e10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 58 a 95 c 113 g 65 t
ORIGIN
1 cttttttttt ttttttgctt gtatcacctg ctgtttattg attgccgcgg gctgtgctct
61 catgggcgac ccatgtcccc aagagcggca ctacatagag gggttggagg aagacggtct
121 gagagaagag gccgcgagag cccaggtgca cagaagcccc ggccccacgg gcctcacgcg
181 ccccaggagg tgtgggcagg gcccgccgcg gagggaggct gcaggtgtgt caggaggcac
241 ctggccggag gtcaccccct ccttctggac cctcagcaga gttgaggcgc ctgcacccca
301 ggggagccag gctgctatct aaaggagtgt g
//
LOCUS BF726108 337 bp mRNA linear EST 05-JAN-2001
DEFINITION by01b12.x1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by01b12 3', mRNA sequence.
ACCESSION BF726108
VERSION BF726108.1 GI:12042019
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 337)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 01 row: b column: 12 Seq primer: -21M13 forward primer
(ABI).
FEATURES Location/Qualifiers
source 1..337
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by01b12"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 58 a 95 c 113 g 71 t
ORIGIN
1 cttttttttt tttttttttt ttgcttgtat cacctgctgt ttattgattg ccgcgggctg
61 tgctctcatg ggcgacccat gtccccaaga gcggcactac atagaggggt tggaggaaga
121 cggtctgaga gaagaggccg cgagagccca ggtgcacaga agccccggcc ccacgggcct
181 cacgcgcccc aggaggtgtg ggcagggccc gccgcggagg gaggctgcag gtgtgtcagg
241 aggcacctgg ccggaggtca ccccctcctt ctggaccctc agcagagttg aggcgcctgc
301 accccagggg agccaggctg ctatctaaag gagtgtg
//
LOCUS BF726318 308 bp mRNA linear EST 05-JAN-2001
DEFINITION by04h03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by04h03 5', mRNA sequence.
ACCESSION BF726318
VERSION BF726318.1 GI:12042229
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 308)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 04 row: h column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..308
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by04h03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 50 a 115 c 72 g 71 t
ORIGIN
1 cgcgatctag cccagaggcc ccgctgactc ctgccagcct ccaggtcccc gtggtaccaa
61 agctgaacat ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct
121 accccagccg gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc
181 ccttcctgtc gtccaccatc agcccctact accgccagtc cctcttccgc accgtgctgg
241 actccggcat ctctgaggtt cgatccgacc tggacaagtt cgtcatcttc ctcgatgtga
301 agcacttc
//
LOCUS BF727103 350 bp mRNA linear EST 05-JAN-2001
DEFINITION by16g06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by16g06 5', mRNA sequence.
ACCESSION BF727103
VERSION BF727103.1 GI:12043014
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 350)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 16 row: g column: 06 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..350
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by16g06"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 109 a 101 c 89 g 50 t 1 others
ORIGIN
1 ggtgcaggcg cctcaactct gctgagggtc cagaaggagg gggtgacctc cggccaggtg
61 cctcctgaca cacctgcagc ctccctccgc ggcgggccct gcccacacct cctggggcgc
121 gtgaggcccg tggggccggg gcttctgtgc acctgggctc tcgcggcctc ttctctcaga
181 ccgtcttcct ccaacccctc tatgtagtgc cgctcttggg gacatgggtc gcccatgaga
241 gcgcagcccg cggcaatcaa taaacagcag gtgatacang ccaaaaaaaa aaaaaaaaaa
301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
//
LOCUS BF727421 312 bp mRNA linear EST 05-JAN-2001
DEFINITION by21b11.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by21b11 5', mRNA sequence.
ACCESSION BF727421
VERSION BF727421.1 GI:12043332
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 312)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 21 row: b column: 11 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..312
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by21b11"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 78 a 99 c 86 g 49 t
ORIGIN
1 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct
61 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg
121 cccgtggggc cggggcttct gtgcacctgg gctctcgcgg cctcttctct cagaccgtct
181 tcctccaacc cctctatgta gtgccgctct tggggacatg ggtcgcccat gagagcgcag
241 cccgcggcaa tcaataaaca gcaggtgata caagcaaaaa aaaaaaaaaa aaaaaaaaaa
301 aaaaaaaaaa ag
//
LOCUS BF726810 289 bp mRNA linear EST 05-JAN-2001
DEFINITION by12d03.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by12d03 5', mRNA sequence.
ACCESSION BF726810
VERSION BF726810.1 GI:12042721
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 289)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 12 row: d column: 03 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..289
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by12d03"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 81 a 90 c 72 g 44 t 2 others
ORIGIN
1 gtgacctccg gccaggtgcc tcctgacaca cctgcagcct ccctccgcgg cgggccctgc
61 ccacacctcc tggggcgcgt gaggcccgtg gggccggggc ttctgtgcac ctgggctctc
121 gcggcctctt ctctcagacc gtcttcctcc aacccctcta tgtagtgccg ctcttgggga
181 catgggtcgc ccatgagagc gcagcccgcg gcaatcaata aacagcaggt gatacangcn
241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagg
//
LOCUS BF726796 218 bp mRNA linear EST 05-JAN-2001
DEFINITION by12b02.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by12b02 5', mRNA sequence.
ACCESSION BF726796
VERSION BF726796.1 GI:12042707
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 218)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 12 row: b column: 02 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..218
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by12b02"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 34 a 85 c 49 g 50 t
ORIGIN
1 cccagaggcc ccgctgactc ctgccagcct ccaggtcccc gtggtaccaa agctgaacat
61 ggatgtgacc atccagcacc cctggttcaa gcgcaccctg gggcccttct accccagccg
121 gctgttcgac cagtttttcg gcgagggcct ttttgagtat gacctgctgc ccttcctgtc
181 gtccaccatc agcccctact accgccagtc cctttttc
//
LOCUS BF726222 559 bp mRNA linear EST 05-JAN-2001
DEFINITION by03c10.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by03c10 5', mRNA sequence.
ACCESSION BF726222
VERSION BF726222.1 GI:12042133
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 559)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 03 row: c column: 10 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..559
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by03c10"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 87 a 188 c 161 g 123 t
ORIGIN
1 catggatgtg accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag
61 ccggctgttc gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct
121 gtcgtccacc atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg
181 catctctgag gtaagacgtg gcccgtggtg ctggcctctc ctcgctgctc agagggtggt
241 ggcctcggtg ggtggagagc gatggactct ggtcttgctc cgtcaggcag gtggcctcgt
301 cccacttcat ccccttgcag aggctgggcg agagcctgtg tccccactgc agccacgtgg
361 cagagcttcc cctggcactg gggagagggt ggacaaggga gcagcctgaa tccacctttg
421 ctttcctcca tcagctcatg acccatgtgt gctttgtaag gcaccagcca catactggaa
481 accccaagag cagcccatcc aggcatgcgt ggtggcgaat gccagctccc gggttcctct
541 ggtctcctga gtcccggag
//
LOCUS BF726205 155 bp mRNA linear EST 05-JAN-2001
DEFINITION by02h06.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by02h06 5', mRNA sequence.
ACCESSION BF726205
VERSION BF726205.1 GI:12042116
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 155)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 02 row: h column: 06 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..155
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by02h06"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 59 a 41 c 31 g 24 t
ORIGIN
1 cgcggcctct tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg
61 acatgggtcg cccatgagag cgcagcccgc ggcaatcaat aaacagcagg tgatacaagc
121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaag
//
LOCUS BF726322 474 bp mRNA linear EST 05-JAN-2001
DEFINITION by04h07.y1 Human Lens cDNA (Un-normalized, unamplified): BY Homo
sapiens cDNA clone by04h07 5', mRNA sequence.
ACCESSION BF726322
VERSION BF726322.1 GI:12042233
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 474)
AUTHORS Wistow,G.J., Bernstein,S., Behal,A. and Smith,D.
TITLE NEIBANK: EST analysis and bioinformatics for ocular genomics
JOURNAL Invest. Ophthalmol. Vis. Sci. 41 (2000) In press
COMMENT Contact: Wistow G Section on Molecular Structure and Function
National Eye Institute 6/331, NIH, Bethesda, MD 20892-2740, USA
Tel: 301 402 3452 Fax: 301 496 0078 Email: graeme@helix.nih.gov
Plate: 04 row: h column: 07 Seq primer: M13RP1 reverse primer
(ABI).
FEATURES Location/Qualifiers
source 1..474
/db_xref="taxon:9606"
/tissue_type="Lens"
/clone="by04h07"
/lab_host="EMDH10B"
/dev_stage="Adult"
/organism="Homo sapiens"
/clone_lib="Human Lens cDNA (Un-normalized, unamplified):
BY"
/note="Organ: Eye; Vector: pCMVSPORT6; Two human lenses
from different adults (both approximately 40 years old)
together yielded 20ug of total RNA and 150ng mRNA for
cDNA library synthesis. A directionally cloned cDNA
library in the pCMVSPORT6 vector was constructed at Life
Technologies , essentially following the protocols of the
SuperScript Plasmid System full details of which are
contained in the manufacturer's Instruction manual
(http://www.lifetech.com/). First strand synthesis was
carried out using a Not I primer-adapter
[5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3']. Not I/blunt
end inserts were cloned into the Not I/EcoR V sites in
the vector. EST analysis was performed on the unamplified
library at the NIH Intramural Sequencing Center (NISC)."
BASE COUNT 238 a 80 c 86 g 70 t
ORIGIN
1 cgcggcctct tctctcagac cgtcttcctc caacccctct atgtagtgcc gctcttgggg
61 acatgggtcg cccatgagag cacagcccgc ggcaatcaat aaacagcagg tgatacaaaa
121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaggggg ggccctttta aaaaaacccc
301 cgggggggcc aaatttttcc cccccccctt ttttttgaaa aaagggcccc ctttgggggg
361 ggtatataaa accagggcca ggccccggtt taaaaaaccc ttggggggga aaaaattttt
421 tgggggaatt tttggaaaaa accccttttt tgggggggga aaaaaatggg aaaa
481
//
LOCUS BI762427 927 bp mRNA linear EST 25-SEP-2001
DEFINITION 603048814F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5189115 5',
mRNA sequence.
ACCESSION BI762427
VERSION BI762427.1 GI:15754005
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 927)
AUTHORS NIH-MGC http://mgc.nci.nih.gov/.
TITLE National Institutes of Health, Mammalian Gene Collection (MGC)
JOURNAL Unpublished (1999)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc. cDNA Library
Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The
I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics,
Inc. Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov Plate: LLAM11472 row: k column: 04 High
quality sequence stop: 809.
FEATURES Location/Qualifiers
source 1..927
/db_xref="taxon:9606"
/clone="IMAGE:5189115"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NIH_MGC_116"
/note="Organ: pooled colon, kidney, stomach; Vector:
pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA
source anonymous pool of 3 colons, age 26 yo male, 49 yo
female, 71 yo male colon; 46 yo male kidney, and pool of
2 stomachs, 62 yo male and 70 yo female. Library is
oligo-dT primed and directionally cloned (EcoRV site is
destroyed upon cloning). Average insert size 1.4 kb,
insert size range 1-3 kb. Library is normalized and
enriched for full-length clones and was constructed by C.
Gruber (Invitrogen). Research Genetics tracking code
023. Note: this is a NIH_MGC Library."
BASE COUNT 168 a 310 c 278 g 171 t
ORIGIN
1 gcaaacaacc agccggccac cagtagcatt cagcccctca gctggccctg agggttggag
61 acaggtcgag ggtcatgcct gtctgtccag gagacagtca caggcccccg aaagctctgc
121 cccacttggt gtgtgggaga agaggccggc aggtgaccga agcatctctg ttctgataac
181 cgggacccgc cctgtctctg ccaaccccag cagggacggc accctctggg cagctccaca
241 tggcacgttg gatttcaggt tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg
301 aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt ggagatccac
361 ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt ccaccggcgc
421 taccgctgcg tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat
481 gctgaccttc tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc
541 catccccgtg tcgcgggagg agaagcccac ctcggctcct cgtcctaagc aggcattgcc
601 tcggctggtc ccctgcagcc cctggcccat catgggggga gcaccctgag ggcggggtgt
661 ctgtcttcct ttgcttccct tttttccttt ccaccttctc acatggaatg agggttgaga
721 gagcagcagg agagcttaag gtctcagggt gtcccagacc cgacacggca gggcggaagt
781 gaccgcacct cacactcctt tagatagcag ctggtcccct ggggtgcagg gcctcaactc
841 tgctgagggt ccggaagagg gggtgacctc cggcagggcc tccgacacac cgcggccccc
901 gcgggggggc ctgccacacc tctgggc
//
LOCUS BI762060 811 bp mRNA linear EST 25-SEP-2001
DEFINITION 603049231F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5189551 5',
mRNA sequence.
ACCESSION BI762060
VERSION BI762060.1 GI:15753638
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 811)
AUTHORS NIH-MGC http://mgc.nci.nih.gov/.
TITLE National Institutes of Health, Mammalian Gene Collection (MGC)
JOURNAL Unpublished (1999)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc. cDNA Library
Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The
I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics,
Inc. Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov Plate: LLAM11473 row: m column: 08 High
quality sequence stop: 746.
FEATURES Location/Qualifiers
source 1..811
/db_xref="taxon:9606"
/clone="IMAGE:5189551"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NIH_MGC_116"
/note="Organ: pooled colon, kidney, stomach; Vector:
pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA
source anonymous pool of 3 colons, age 26 yo male, 49 yo
female, 71 yo male colon; 46 yo male kidney, and pool of
2 stomachs, 62 yo male and 70 yo female. Library is
oligo-dT primed and directionally cloned (EcoRV site is
destroyed upon cloning). Average insert size 1.4 kb,
insert size range 1-3 kb. Library is normalized and
enriched for full-length clones and was constructed by C.
Gruber (Invitrogen). Research Genetics tracking code
023. Note: this is a NIH_MGC Library."
BASE COUNT 147 a 260 c 238 g 166 t
ORIGIN
1 cagctggcct gagggtttgg agacaggtcg agggtcatgc ctgtctgtcc aggagacagt
61 cacaggcccc cgaaagctct gccccacttg gtgtgtggga gaagaggccg gcaggtgacc
121 gaagcatctc tgttctgata accgggaccc gccctgtctc tgccaacccc agcagggacg
181 gcaccctctg ggcagctcca catggcacgt ttggatttca ggttcgatcc gaccgggaca
241 agttcgtcat cttcctcgat gtgaagcact tctccccgga ggacctcacc gtgaaggtgc
301 aggacgactt tgtggagatc cacggaaagc acaacgagcg ccaggacgac cacggctaca
361 tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag tcggccctct
421 cttgctccct gtctgccgat ggcattgctg accttctgtg gccccaagat ccagactggc
481 ctggatgcca cccacgccga gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg
541 gctccctcgt cctaagcagg cattgcctcg gctggctccc ctgcaagccc tgtgccatca
601 ttggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc
661 caccttctca cattggaatg aggggttgag agagcagcca ggagagctta gggtctcagg
721 ggtgtccaga cccgacacgg ccagtggcgg aagtgaacgg acctcacatc ctttagatag
781 cagcttggtc ccttggggtt caaggcgccc a
//
LOCUS BI765128 862 bp mRNA linear EST 25-SEP-2001
DEFINITION 603051343F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5191307 5',
mRNA sequence.
ACCESSION BI765128
VERSION BI765128.1 GI:15756706
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 862)
AUTHORS NIH-MGC http://mgc.nci.nih.gov/.
TITLE National Institutes of Health, Mammalian Gene Collection (MGC)
JOURNAL Unpublished (1999)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc. cDNA Library
Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The
I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics,
Inc. Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov Plate: LLAM11478 row: f column: 12 High
quality sequence stop: 456.
FEATURES Location/Qualifiers
source 1..862
/db_xref="taxon:9606"
/clone="IMAGE:5191307"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NIH_MGC_116"
/note="Organ: pooled colon, kidney, stomach; Vector:
pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA
source anonymous pool of 3 colons, age 26 yo male, 49 yo
female, 71 yo male colon; 46 yo male kidney, and pool of
2 stomachs, 62 yo male and 70 yo female. Library is
oligo-dT primed and directionally cloned (EcoRV site is
destroyed upon cloning). Average insert size 1.4 kb,
insert size range 1-3 kb. Library is normalized and
enriched for full-length clones and was constructed by C.
Gruber (Invitrogen). Research Genetics tracking code
023. Note: this is a NIH_MGC Library."
BASE COUNT 209 a 282 c 271 g 100 t
ORIGIN
1 cctgagggcg gggtgtctgt cttcctttgc ttcccttttt tcctttccac cttctcacag
61 tggaatgagg gttgagagag cagccaggag agcttagggt ctcagggtgt cccagacccc
121 gacaccggcc agtggcggaa gtgaccgcac ctcacactcc tttagatagc agcctggctc
181 ccctggggtg caggcgcctc aactctgctg agggtccaga aggagggggt gacctccggc
241 caggtgcctc ctgacacacc tgcagcctcc ctccgcggcg ggccctgccc acacctcctg
301 gggcgcgtga ggcccgtggg gccggggctt ctgtgcacct gggctctcgc ggcctcttct
361 ctcagaccgt cttcctccaa cccctctatg tagtgccgct cttggggaca tgggtcgccc
421 atgagagcgc agccgcggaa tcaataaaca gcaggtgact acaaggcaaa ggcaccacga
481 gacacaaaga agacagacaa caaacagaag aagaaggagc aaccacgcag cagagagccg
541 ggcagaggcg gggcccccag acagcaccgc cccccggggg acacaagtaa gaacgatggc
601 acgagaagca cgcggcagac accgcgagga acaaaatggg gagcagggag cgagaagcca
661 tagacacaga ccagagacgc cgcagagcga gccgccccgg gcgacgcccg cgcccgcacg
721 gcggcaagac aggggcacag acgagaagac gagacgagag acgggaacac acgggcgacc
781 gcgcccccgg atccgaacac cgaccaacga aggcgcgcag cagcgacgaa gacgaaaaca
841 aaagccccag cgccagacac ca
//
LOCUS BI761339 753 bp mRNA linear EST 25-SEP-2001
DEFINITION 603043909F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5184266 5',
mRNA sequence.
ACCESSION BI761339
VERSION BI761339.1 GI:15752917
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 753)
AUTHORS NIH-MGC http://mgc.nci.nih.gov/.
TITLE National Institutes of Health, Mammalian Gene Collection (MGC)
JOURNAL Unpublished (1999)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc. cDNA Library
Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The
I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics,
Inc. Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov Plate: LLAM11460 row: a column: 03 High
quality sequence stop: 723.
FEATURES Location/Qualifiers
source 1..753
/db_xref="taxon:9606"
/clone="IMAGE:5184266"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NIH_MGC_116"
/note="Organ: pooled colon, kidney, stomach; Vector:
pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA
source anonymous pool of 3 colons, age 26 yo male, 49 yo
female, 71 yo male colon; 46 yo male kidney, and pool of
2 stomachs, 62 yo male and 70 yo female. Library is
oligo-dT primed and directionally cloned (EcoRV site is
destroyed upon cloning). Average insert size 1.4 kb,
insert size range 1-3 kb. Library is normalized and
enriched for full-length clones and was constructed by C.
Gruber (Invitrogen). Research Genetics tracking code
023. Note: this is a NIH_MGC Library."
BASE COUNT 138 a 255 c 207 g 153 t
ORIGIN
1 cttatctata accagccacc tgaccatagc caaacaacca gccggcccac cagtagcatt
61 cagcccctca gctggccctg agggtttgga gacaggtcga gggtcatgcc tgtctgtcca
121 ggagacagtc acaggccccc gaaagctctg ccccacttgg tgtgtgggag aagaggccgg
181 caggtgaccg aagcatctct gttctgataa ccgggacccg ccctgtctct gccaacccca
241 gcagggacgg caccctctgg gcagctccac atggcacgtt tggatttcag gttcgatccg
301 accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag gacctcaccg
361 tgaaggtgca ggacgacttt gtggagatcc acgggaaagc acgaacgagc gccaggacga
421 ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca acgtggacca
481 gtcggtcctc tcttgctccc tgtctgccga tggcatgctg accttctgtg gccccaagat
541 ccagactggc ctggatgcca tccacgccga gcgagccatc cccgtgtcgc gggagggaga
601 agcccagctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc
661 tggcccatca tggggggagc acccttgatg ggcggggtgt ctgtcttcct ttgcttcccg
721 ttttgacctt tccaccttct cacattggaa ctg
//
LOCUS BM924704 1171 bp mRNA linear EST 12-MAR-2002
DEFINITION AGENCOURT_6653675 NIH_MGC_116 Homo sapiens cDNA clone
IMAGE:5761132 5', mRNA sequence.
ACCESSION BM924704
VERSION BM924704.1 GI:19375083
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1171)
AUTHORS NIH-MGC http://mgc.nci.nih.gov/.
TITLE National Institutes of Health, Mammalian Gene Collection (MGC)
JOURNAL Unpublished (1999)
COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc. cDNA Library
Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The
I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Agencourt
Bioscience Corporation Clone distribution: MGC clone distribution
information can be found through the I.M.A.G.E. Consortium/LLNL
at: http://image.llnl.gov Plate: LLAM12809 row: e column: 05 High
quality sequence start: 8 High quality sequence stop: 760.
FEATURES Location/Qualifiers
source 1..1171
/db_xref="taxon:9606"
/clone="IMAGE:5761132"
/lab_host="DH10B"
/organism="Homo sapiens"
/clone_lib="NIH_MGC_116"
/note="Organ: pooled colon, kidney, stomach; Vector:
pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA
source anonymous pool of 3 colons, age 26 yo male, 49 yo
female, 71 yo male colon; 46 yo male kidney, and pool of
2 stomachs, 62 yo male and 70 yo female. Library is
oligo-dT primed and directionally cloned (EcoRV site is
destroyed upon cloning). Average insert size 1.4 kb,
insert size range 1-3 kb. Library is normalized and
enriched for full-length clones and was constructed by C.
Gruber (Invitrogen). Research Genetics tracking code
023. Note: this is a NIH_MGC Library."
BASE COUNT 230 a 394 c 323 g 222 t 2 others
ORIGIN
1 gtccggaatt ctccggatgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc
61 cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg
121 agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt
181 ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct
241 gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt
301 cgcgggagga gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct
361 cccctgcagc cctggcccat catgggggga gcaccctgag ggcggggtgt ctgtcttcct
421 ttgcttccct tttttccttt ccaccttctc acatggaatg agggtttgag agagcagcca
481 ggagagctta gggtctcagg gtgtcccaga ccccgacacc ggccagtggc ggaagtgacc
541 gcacctcaca ctcctttaga tagcagcctg gctcccctgg ggtgcaggcg cctcaactct
601 gctgagggtc cagaaggagg gggtgacctc cggccaggtg cctcctgaca cacctgcagc
661 ctccctccgc ggcgggccct gcccacacct cctggggcgc gtgaggcccg tggggccggg
721 gcttctgtgc acctgggctc tcgcggcctc ttctctcaga ccgtcttcct ccaacccctc
781 tatgtagtgc cgctcttggg gacatgggtc gcccatgaga gcgcagcccg cggcaatcaa
841 taaacagcag gttgatacaa ggcaaanaga cccaacaaca aacgaaactg cgttcagctc
901 acgtccgatc ccaagcgaat ctactgcccc actcctttat agcancaaag gaggggcggc
961 cccctttaaa aaatcccccc agaggggggc ccagacttta acgggaaacc ccggttttct
1021 ttggaacaaa agggggcccc tcataaggag agcgcgtata ttaaaacaag ggaacgcggc
1081 cccgccgttc tttatacaaa ccccgcggca tggggggaaa accatccttt gcccttgcga
1141 gacactcttt cgcggagaaa gacgcccctc c
//
LOCUS BM679127 810 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-ahy-o-03-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-o-03-0-UI 3', mRNA sequence.
ACCESSION BM679127
VERSION BM679127.1 GI:18989023
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 810)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..810
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-o-03-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 152 a 231 c 276 g 149 t 2 others
ORIGIN
1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg
541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg
601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg
661 cagacaggga gcaagagagg gccgactggt ccacgttgga cggcaggcgg tancggcggt
721 ggaactcacg ggaaatgtan ccgtggtcgt cctggcgctc gttgtgcttt ccgtggatct
781 ccacaaagtc gtcctgcact tcacggtgag
//
LOCUS BM671973 775 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-ahz-g-22-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahz-g-22-0-UI 3', mRNA sequence.
ACCESSION BM671973
VERSION BM671973.1 GI:18981871
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 775)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..775
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahz-g-22-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 144 a 220 c 268 g 141 t 2 others
ORIGIN
1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg
541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg
601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg
661 cagacaggga gcaagagagg gccgactggt ccacgttgga cggcaggcgg tancggcggt
721 ggaactcacg ggaaatgtan ccgtggtcgt cctggcgctc gttgtgcttt ccgtg
781
//
LOCUS BM722336 724 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahx-i-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahx-i-06-0-UI 5', mRNA sequence.
ACCESSION BM722336
VERSION BM722336.1 GI:19042842
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 724)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..724
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahx-i-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 120 a 259 c 189 g 155 t 1 others
ORIGIN
1 ccccgctgac tcctgccagc ctccaggtcc ccgtggtacc aaagctgaac atggacgtga
61 ccatccagca cccctggttc aagcgcaccc tggggccctt ctaccccagc cggctgttcg
121 accagttttt cggcgagggc ctttttgagt atgacctgct gcccttcctg tcgtccacca
181 tcagccccta ctaccgccag tccctcttcc gcaccgtgct ggactccggc atctctgagg
241 ttcgatccga ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccggagg
301 acctcaccgt gaaggtgcag gacgactttg tggagatcca cggaaagcac aacgagcgcc
361 aggacgacca cggctacatt tcccgtgagt tccaccgccg ctaccgcctg ccgtccaacg
421 tggaccagtc ggccctctct tgctcnctgt ctgccgatgg catgctgacc ttctgtggcc
481 ccaagatcca gactggcctg gatgccaccc acgccgagcg agccatcccc gtgtcgcggg
541 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg
601 cagccctggc ccatcatggg gggagcaccc tgagggcggg gtgtctgtct tcctttgctt
661 cccttttttc ctttccacct tctcacatgg aatgagggtt tgagagagca gccaggagag
721 ctta
//
LOCUS BM721044 703 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-aia-i-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-aia-i-06-0-UI 5', mRNA sequence.
ACCESSION BM721044
VERSION BM721044.1 GI:19040715
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 703)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..703
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-aia-i-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 115 a 247 c 206 g 133 t 2 others
ORIGIN
1 ccgggacaag ttcgtcatct tcctcgatgt gaagcacttc tccccnggag gacctcaccg
61 tgaaggtgca ggacgacttt ggtggagatc cacggaaagc acaacgagcg ccaggacgac
121 cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa cgtggaccag
181 tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg ccccaagatc
241 cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgcg ggaggagaag
301 cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg ctggctcccc tgcagccctg
361 gcccatcatg gggggagcac cctgagggcg gggtgtctgt cttcctttgc ttcccttttt
421 tcctttccac cttctcacat ggaatgaggg tttgagagag cagccaggag agcttagggt
481 ctcagggtgt cccagacccc gacaccggcc agtggcggaa gtgaccgcac ctcacactcc
541 tttagatagc agcctggctc ccctggggtg caggcgcctc aactctgctg agggtccaga
601 aggagggggt gacctccggc caggtgcctc ctgacacacc tgcagcctcc ctccgcggcg
661 ggccctgccc acacctcctg nggcgcgtga ggcccgtggg gcc
//
LOCUS BM671946 702 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-ahz-c-10-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahz-c-10-0-UI 3', mRNA sequence.
ACCESSION BM671946
VERSION BM671946.1 GI:18981844
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 702)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..702
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahz-c-10-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 134 a 202 c 241 g 124 t 1 others
ORIGIN
1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg
541 cttaggacga gggagccgag gtgggcttct cctcccgcga cacggggatg gctcgctcgg
601 cgtgggtggc atccaggcca gtctggatct tggggccaca gaaggtcagc atgccatcgg
661 cagacaggga gcangagagg gccgactggt ccacgttgga cg
//
LOCUS BM696617 697 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-e-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-e-20-0-UI 5', mRNA sequence.
ACCESSION BM696617
VERSION BM696617.1 GI:19009875
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 697)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..697
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-e-20-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 105 a 250 c 203 g 137 t 2 others
ORIGIN
1 cggaaagcac aacgagcgcc aggacgacca cggctacatt tcccgtgagt tccaccgccg
61 ctaccgcctg ccgtccaacg tggaccagtc ggccctctct tgctccctgt ctgccgatgg
121 catgctgacc ttctgtggcc ccaagatcca gactggcctg gatgccaccc acgccgagcg
181 agccatcccc gtgtcgcggg aggagaagcc cacctcggct ccctcgtcct aagcaggcat
241 tgcctcggct ggctcccctg cagccctggc ccatcatggg gggagcaccc tgagggcggg
301 gtgtctgtct tcctttgctt ccctgttttc ctttccacct tctcacatgg aatgagggtt
361 tgagagagca gccaggagag cttagggtct cagggtgtcc cagaccccga caccggccag
421 tggcggaagt gaccgcacct cacactcctt tagatagcag cctggctccc ctggggtgca
481 ggcgcctcaa ctctgctgag ggtccagaag gagggggtga cctccggcca ggtgcctcct
541 gacacacctg cagcctccct ccgcggcggg ccctgcccac acctcctggg gcgcgtgagg
601 cccgtggggc cggggcttct gtgcacctgn gctctcgcgg cctcttctct cagnacgtct
661 tcctcaaccc ctctatgtag tgcgctcttg gggacat
//
LOCUS BM696995 664 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agl-h-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agl-h-20-0-UI 5', mRNA sequence.
ACCESSION BM696995
VERSION BM696995.1 GI:19010253
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 664)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..664
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agl-h-20-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 103 a 235 c 198 g 126 t 2 others
ORIGIN
1 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct
61 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc
121 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg
181 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct
241 cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc ctggcccatc
301 atggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc
361 caccttctca catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg
421 tgtcccagac cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat
481 agcagcctgg ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg
541 ggtgacctcc ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gcgggccctg
601 cccacacctc ctgnggcgcg tgaggcccgt ggggccgggg cttctgtgca cctgngctct
661 cgcg
//
LOCUS BM706237 667 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-c-17-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-c-17-0-UI 5', mRNA sequence.
ACCESSION BM706237
VERSION BM706237.1 GI:19019495
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 667)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..667
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-c-17-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 108 a 236 c 198 g 123 t 2 others
ORIGIN
1 tctccccgga ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc
61 acaacgagcg ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc
121 tgccgtccaa cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga
181 ccttctgtgg ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc
241 ccgtgtcgcg ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg
301 ctggctcccc tgcagccctg gcccatcatg gggggagcac cctgagggcg gggtgtctgt
361 cttcctttgc ttcccttttt tcctttccac cttctcacat ggaatgaggg tttgagagag
421 cagccaggag agcttagggt ctcagggtgt cccagacccc gacaccggcc agtggcggaa
481 gtgaccgcac ctcacactcc tttanatanc agcctggctc ccctggggtg caggcgcctc
541 aactctgctg agggtccaaa aggagggggt gacctccggc caggtgcctc ctgacacacc
601 tgcagcctcc ctccgcggcg ggccctgccc acacctcctg gggcgcgtga ggcccgtggg
661 gccgggg
//
LOCUS BM706012 653 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agg-i-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agg-i-06-0-UI 5', mRNA sequence.
ACCESSION BM706012
VERSION BM706012.1 GI:19019270
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 653)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..653
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agg-i-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 101 a 231 c 196 g 125 t
ORIGIN
1 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc
61 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg
121 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga
181 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc
241 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg
301 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc
361 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca
421 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc
481 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc
541 tccggccagg tgcctcctga cacacctgca gcctccctcc gcggggggcc ctgcccacac
601 ctcctggggc gcgtgaggcc cgtggggccg gggcttctgt gcacctgggc tct
661
//
LOCUS BM697160 661 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-i-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-i-06-0-UI 5', mRNA sequence.
ACCESSION BM697160
VERSION BM697160.1 GI:19010418
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 661)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..661
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-i-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 107 a 235 c 174 g 144 t 1 others
ORIGIN
1 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt
61 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca
121 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg
181 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg
241 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc
301 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca
361 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg
421 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc
481 gggaggagaa gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc
541 ctgcagccct ggcccatcat ggggggagca ccctgagggc ggngtgtctg tcttcctttg
601 cttccctttt ttcctttcac cttctcacat ggaatgaggg tttgagagag cagccaggga
661 g
//
LOCUS BM722793 650 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahy-n-05-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-n-05-0-UI 5', mRNA sequence.
ACCESSION BM722793
VERSION BM722793.1 GI:19043627
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 650)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..650
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-n-05-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 105 a 227 c 195 g 121 t 2 others
ORIGIN
1 cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc gccaggacga
61 ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca acgtggacca
121 gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg gccccaagat
181 ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc gggaggagaa
241 gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc ctgcaaccct
301 ggcccatcat ggggggagca ccctgagggc ggggtgtctg tcttcctttg cttccctttt
361 ttcctttcca ccttctcaca tggaatgagg gtttgagaga gcagccagga gagcttaggg
421 tctcagggtg tcccagaccc cgacaccggc cagtggcgga agtgaccgca cctcacactc
481 ctttagatag cagcctggct cccctggggt gcaggcgcct caactctgct gagggtccag
541 aaggaggggg tgacctccgg ncaggtgcct cctgacacac ctgcagcctc cctccgcggc
601 gggccctgcc cacacctcct gnggcgcgtg aggcccgtgg ggccggggct
//
LOCUS BM686748 663 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-CQ0-adq-b-11-0-UI.r1 UI-E-CQ0 Homo sapiens cDNA clone
UI-E-CQ0-adq-b-11-0-UI 5', mRNA sequence.
ACCESSION BM686748
VERSION BM686748.1 GI:19000006
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 663)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..663
/db_xref="taxon:9606"
/tissue_type="optic nerve"
/clone="UI-E-CQ0-adq-b-11-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-CQ0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-CQ0 is a cDNA library containing the following
tissue(s): optic nerve. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CCATTAAGTG. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 107 a 233 c 176 g 145 t 2 others
ORIGIN
1 gcacgagggg acgtgaccat ccagcacccc tggttcaagc gcaccctggg gcccttctac
61 cccagccggc tgttcgacca gtttttcggc gagggccttt ttgagtatga cctgctgccc
121 ttcctgtcgt ccaccatcag cccctactac cgccagtccc tcttccgcac cgtgctggac
181 tccggcatct ctgaggttcg atccgaccgg gacaagttcg tcatcttcct cgatgtgaag
241 cacttctccc cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga
301 aagcacaacg agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac
361 cgcctgccgt ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg
421 ctgaccttct gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc
481 atccccgtgt cgcgggagga gaagccacct cggctccctc gtcctaagca ggcattgcct
541 cggctggctc ncctgcagcc ctggcccatc atggggggag caccctgagg gcggngtgtc
601 tgtcttcctt tgctttcctt ttttcctttc caccttctca catggaatga gggtttgaga
661 gag
//
LOCUS BM679139 674 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-ahy-a-10-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-a-10-0-UI 3', mRNA sequence.
ACCESSION BM679139
VERSION BM679139.1 GI:18989035
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 674)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..674
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-a-10-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 129 a 194 c 228 g 122 t 1 others
ORIGIN
1 tttttttttt ttttttttgc gggttgcttg tatcacctgc tgtttattga ttgccgcggg
61 ctgcgctctc atgggcgacc catgtcccca agagcggcac tacataaagg ggttggagga
121 agacggtctg agagaagagg ccgcgagagc ccaggtgcac agaagccccg gccccacggg
181 cctcacgcgc cccaggaggt gtgggcaggg cccgccgcgg agggaggctg caggtgtgtc
241 aggaggcacc tggccggagg tcaccccctc cttctggacc ctcagcagag ttgaggcgcc
301 tgcaccccag gggagccagg ctgctatcta aaggagtgtg aggtgcggtc acttccgcca
361 ctggccggtg tcggggtctg ggacaccctg agaccctaag ctctcctggc tgctctctca
421 aaccctcatt ccatgtgaga aggtggaaag gaaaaaaggg aagcaaagga agacagacac
481 cccgccctca gggtgctccc cccatgatgg gccagggctg caggggagcc agccgaggca
541 atgcctgctt aagacgaggg agccgaggtg ggcttctcct cncgcgacac ggggatggct
601 cgctcggcgt gggtggcatc caggccagtc tggatcttgg ggccacagaa ggtcagcatg
661 ccatcggcag acag
//
LOCUS BM706270 659 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-k-09-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-k-09-0-UI 5', mRNA sequence.
ACCESSION BM706270
VERSION BM706270.1 GI:19019528
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 659)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..659
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-k-09-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 108 a 241 c 175 g 134 t 1 others
ORIGIN
1 cagaggcccc gctgactcct gccagcctcc aggtccccgt ggtaccaaag ctgaacatgg
61 atgtgaccat ccagcacccc tggttcaagc gcaccctggg gcccttctac cccagccggc
121 tgttcgacca gtttttcggc gagggccttt ttgagtatga cctgctgccc ttcctgtcgt
181 ccaccatcag cccctactac cgccagtccc tcttccgcac cgtgctggac tccggcatct
241 ctgaggttcg atccgaccgg gacaagttcg tcatcttcct cgatgtgaag cacttctccc
301 cggaggacct caccgtgaag gtgcaggacg actttgtgga gatccacgga aagcacaacg
361 agcgccagga cgaccacggc tacatttccc gtgagttcca ccgccgctac cgcctgccgt
421 ccaacgtgga ccagtcggcc ctctcttgct ccctgtctgc cgatggcatg ctgaccttct
481 gtggccccaa gatccagact ggcctggatg ccacccacgc cgagcgagcc atccccgtgt
541 cgcgggagga gaagcccacc tcggctccct cgtcctaagc aggcattgcc tcggctggct
601 ccctgcagcc cttggccatc atggggggag caccctgagg gcggngtgtc tgtcttcct
661
//
LOCUS BM696872 638 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agl-o-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agl-o-20-0-UI 5', mRNA sequence.
ACCESSION BM696872
VERSION BM696872.1 GI:19010130
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 638)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..638
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agl-o-20-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 109 a 221 c 181 g 126 t 1 others
ORIGIN
1 cgtcatcttc ctcgatgtga agcacttctc cccggaggac ctcaccgtga aggtgcagga
61 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc
121 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg
181 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga
241 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc
301 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg
361 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc
421 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtcnca
481 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc
541 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc
601 tccggccagg tgcctcctga cacacctgca gcctccct
//
LOCUS BM706251 631 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-g-05-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-g-05-0-UI 5', mRNA sequence.
ACCESSION BM706251
VERSION BM706251.1 GI:19019509
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 631)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..631
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-g-05-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 106 a 225 c 170 g 130 t
ORIGIN
1 tgacctgctg cccttcctgt cgtccaccat cagcccctac taccgccagt ccctcttccg
61 caccgtgctg gactccggca tctctgaggt tcgatccgac cgggacaagt tcgtcatctt
121 cctcgatgtg aagcacttct ccccggagga cctcaccgtg aaggtgcagg acgactttgt
181 ggagatccac ggaaagcaca acgagcgcca ggacgaccac ggctacattt cccgtgagtt
241 ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg gccctctctt gctccctgtc
301 tgccgatggc atgctgacct tctgtggccc caagatccag actggcctgg atgccaccca
361 cgccgagcga gccatccccg tgtcgcggga ggagaagccc acctcggctc cctcgtccta
421 agcaggcatt gcctcggctg gctcccctgc agccctggcc catcatgggg ggagcaccct
481 gagggcgggg tgtctgtctt cctttgcttc ccttttttcc tttccacctt ctcacatgga
541 atgagggttt gagagagcag ccaggagagc ttagggtctc agggtgtccc agaccccgac
601 accggccagt ggcggaagtg accgcacctc a
//
LOCUS BM697066 635 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-g-03-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-g-03-0-UI 5', mRNA sequence.
ACCESSION BM697066
VERSION BM697066.1 GI:19010324
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 635)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..635
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-g-03-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 99 a 233 c 160 g 141 t 2 others
ORIGIN
1 gatgtgacca tccagcaccc ctggttcaag cgcaccctgg ggcccttcta ccccagccgg
61 ctgttcgacc agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg
121 tccaccatca gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc
181 tctgaggttc gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc
241 ccggaggacc tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac
301 gagcgccagg acgaccacgg ctacatttcc cgtgagttcc accgccgcta ccgcctgccg
361 tccaacgtgg accagtcggc cctctcttgc tccctgtctg ccgatggcat gctgaccttc
421 tgtggcccca agatccagac tggcctggat gccacccacg ccgagcgagc catccccgtg
481 tcgcgggagg agaagcccac ctcggctccc tcgtcctaag caggcattgc ctcggctggc
541 tcccctgcag ccctggccca tcatggnggg agcaccctga gggcggngtg tctgtcttcc
601 tttgcttccc ttttttcctt tccaccttct cacat
//
LOCUS BM696651 630 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-k-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-k-24-0-UI 5', mRNA sequence.
ACCESSION BM696651
VERSION BM696651.1 GI:19009909
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 630)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..630
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-k-24-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 106 a 233 c 166 g 125 t
ORIGIN
1 aggccccgct gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggatg
61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt
121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca
181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg
241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg
301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc
361 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca
421 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg
481 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc
541 gggaggagaa gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc
601 ctgcagccct gggccatcat ggggggagca
//
LOCUS BM696679 615 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-b-21-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-b-21-0-UI 5', mRNA sequence.
ACCESSION BM696679
VERSION BM696679.1 GI:19009937
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 615)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..615
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-b-21-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 99 a 220 c 179 g 117 t
ORIGIN
1 cgactttgtg gagatccacg gaaagcacaa cgagcgccag gacgaccacg gctacatttc
61 ccgtgagttc caccgccgct accgcctgcc gtccaacgtg gaccagtcgg ccctctcttg
121 ctccctgtct gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga
181 tgccacccac gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc
241 ctcgtcctaa gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg
301 gagcaccctg agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc
361 tcacatggaa tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca
421 gaccccgaca ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc
481 tggctcccct ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc
541 tccggccagg tgcctcctga cacacctgca gcctccctcc gcggcgggcc ctgcccacac
601 ctcctggggc gcgtg
//
LOCUS BM721028 642 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-aia-e-06-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-aia-e-06-0-UI 5', mRNA sequence.
ACCESSION BM721028
VERSION BM721028.1 GI:19040678
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 642)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..642
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-aia-e-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 102 a 229 c 167 g 143 t 1 others
ORIGIN
1 accatccagc acccctggtt caagcgcacc ctgggggccc ttctacccca gccggctgtt
61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac
121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga
181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga
241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg
301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa
361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggcatgctga ccttctgtgg
421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagcatccc cgtgtcgcgg
481 gaggagaagc cacctcggct ccctcgtcct aagcaggcat tgcctcggct ggctcccctg
541 cagccctggc ccatcatggg gggagaccct gagggcgggg tgtctgtctt cctttgcttc
601 ncttttttcc tttccacctt ctcacatgga atgagggttt ga
//
LOCUS BM697176 618 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-k-18-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-k-18-0-UI 5', mRNA sequence.
ACCESSION BM697176
VERSION BM697176.1 GI:19010434
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 618)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..618
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-k-18-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 106 a 209 c 174 g 126 t 3 others
ORIGIN
1 ccggcatctc tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc
61 acttctcccc ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa
121 agcacaacga gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc
181 gcctgccgtc caacgtggac cagtcggccc tctcttgctc cctgtctgcc gatggcatgc
241 tgaccttctg tggccccaag atccagactg gcctggatgc cacccacgcc gagcgagcca
301 tccccgtgtc gcgggaggag aagcccacct cggctccctc gtcctaagca ggcattgcct
361 cggctggctc ccctgcagcc ctggcccatc atggggggag caccctgagg gcggngtgtc
421 tgtcttcctt tgcttcnctt ttttcctttc caccttctca catggaatga gggtttgaga
481 gagcagccag gagagcttag ggtctcaggg tgtcncagac cccgacaccg gccagtggcg
541 gaagtgaccg cacctcacac tcctttagat agcagcctgg ctcccctggg gtgcaggcgc
601 ctcaactctg ctgagggt
//
LOCUS BM679458 622 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-aia-e-06-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-aia-e-06-0-UI 3', mRNA sequence.
ACCESSION BM679458
VERSION BM679458.1 GI:18989354
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 622)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..622
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-aia-e-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 116 a 182 c 213 g 111 t
ORIGIN
1 tttttttttt ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca
61 tgggcgaccc atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga
121 gagaagaggc cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc
181 ccaggaggtg tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct
241 ggccggaggt caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg
301 ggagccaggc tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt
361 cggggtctgg gacaccctga gaccctaagc tctcctggct gctctctcaa accctcattc
421 catgtgagaa ggtggaaagg aaaaaaggga agcaaaggaa gacagacacc ccgccctcag
481 ggtgctcccc ccatgatggg ccagggctgc aggggagcca gccgaggcaa tgcctgctta
541 ggacgaggga gccgaggtgg gcttctcctc tcgcgacacg gggatggctc gctcggcgtg
601 ggtggcatcc aggccagtct gg
//
LOCUS BM678406 616 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-EO0-ahw-c-15-0-UI.s1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahw-c-15-0-UI 3', mRNA sequence.
ACCESSION BM678406
VERSION BM678406.1 GI:18988302
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 616)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..616
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahw-c-15-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI). TAG_LIB=UI-E-EO0 TAG_TISSUE=human fetal eye
TAG_SEQ=CGCGTATACC"
BASE COUNT 116 a 182 c 210 g 108 t
ORIGIN
1 tttttttttt ttttgcttgt atcacctgct gtttattgat tgccgcgggc tgcgctctca
61 tgggcgaccc atgtccccaa gagcggcact acatagaggg gttggaggaa gacggtctga
121 gagaagaggc cgcgagagcc caggtgcaca gaagccccgg ccccacgggc ctcacgcgcc
181 ccaggaggtg tgggcagggc ccgccgcgga gggaggctgc aggtgtgtca ggaggcacct
241 ggccggaggt caccccctcc ttctggaccc tcagcagagt tgaggcgcct gcaccccagg
301 ggagccaggc tgctatctaa aggagtgtga ggtgcggtca cttccgccac tggccggtgt
361 cggggtctgg gacaccctga gaccctaagc tctcctggct gctctctcaa accctcattc
421 catgtgagaa ggtggaaagg aaaaaaggga agcaaaggaa gacagacacc ccgccctcag
481 ggtgctcccc ccatgatggg ccagggctgc aggggagcca gccgaggcaa tgcctgctta
541 ggacgaggga gccgaggtgg gcttctcctc ccgcgacacg gggatggctc gctcggcgtg
601 ggtggcatcc aggcca
//
LOCUS BM696976 599 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agl-f-04-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agl-f-04-0-UI 5', mRNA sequence.
ACCESSION BM696976
VERSION BM696976.1 GI:19010234
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 599)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..599
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agl-f-04-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 98 a 208 c 161 g 131 t 1 others
ORIGIN
1 ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat cagcccctac
61 taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt tcgatccgac
121 cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga cctcaccgtg
181 aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca ggacgaccac
241 ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt ggaccagtcg
301 gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc caagatccag
361 actggcctgg atgccaccca cgccgagcga gccatccccg tgtcgcggga ggagaagccc
421 acctcggctc cctcgtccta agcaggcatt gcctcggctg gctcccctgc agccctggcc
481 catcatgggg ggagcaccct gagggcggng tgtctgtctt cctttgcttc ccttttttcc
541 tttccacctt ctcacatgga atgagggttt gagagagcag ccaggagagc ttagggtct
601
//
LOCUS BM696670 607 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-o-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-o-16-0-UI 5', mRNA sequence.
ACCESSION BM696670
VERSION BM696670.1 GI:19009928
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 607)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..607
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-o-16-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 100 a 211 c 160 g 135 t 1 others
ORIGIN
1 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca
61 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg
121 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg
181 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc
241 gccaggacga ccacggctac atttcccgtg aagttccacc gccgctaccg cctgccgtcc
301 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt
361 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg
421 cgggaggaga agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc
481 cctgcagccc tggcccatca tggggggagc accctgaggg cggngtgtct gtcttccttt
541 gcttcccttt tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg
601 agagctt
//
LOCUS BM697101 593 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-m-13-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-m-13-0-UI 5', mRNA sequence.
ACCESSION BM697101
VERSION BM697101.1 GI:19010359
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 593)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..593
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-m-13-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 93 a 218 c 159 g 123 t
ORIGIN
1 cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag
61 tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc
121 ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga
181 tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc
241 accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac
301 gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac
361 cagtcggccc tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag
421 atccagactg gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag
481 aagcccacct cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc
541 ctggcccatc atggggggag caccctgagg gcggggtgtc tgtcttcctt tgc
601
//
LOCUS BM705926 592 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-f-18-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-f-18-0-UI 5', mRNA sequence.
ACCESSION BM705926
VERSION BM705926.1 GI:19019184
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 592)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..592
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-f-18-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 100 a 221 c 152 g 119 t
ORIGIN
1 gccccgctga ctcctgccag cctccaggtc cccgtggtac caaagctgaa catggacgtg
61 accatccagc acccctggtt caagcgcacc ctggggccct tctaccccag ccggctgttc
121 gaccagtttt tcggcgaggg cctttttgag tatgacctgc tgcccttcct gtcgtccacc
181 atcagcccct actaccgcca gtccctcttc cgcaccgtgc tggactccgg catctctgag
241 gttcgatccg accgggacaa gttcgtcatc ttcctcgatg tgaagcactt ctccccggag
301 gacctcaccg tgaaggtgca ggacgacttt gtggagatcc acggaaagca caacgagcgc
361 caggacgacc acggctacat ttcccgtgag ttccaccgcc gctaccgcct gccgtccaac
421 gtggaccagt cggccctctc ttgctccctg tctgccgatg gcatgctgac cttctgtggc
481 cccaagatcc agactggcct ggatgccacc cacgccgagc gagccatccc cgtgtcgcgg
541 gaggagaagc ccacctcggc tccctcgtcc taagcaggca ttgcctcggc tg
601
//
LOCUS BM696581 596 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-o-15-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-o-15-0-UI 5', mRNA sequence.
ACCESSION BM696581
VERSION BM696581.1 GI:19009839
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 596)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..596
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-o-15-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 102 a 220 c 154 g 120 t
ORIGIN
1 agaggccccg ctgactcctg ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga
61 tgtgaccatc cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct
121 gttcgaccag tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc
181 caccatcagc ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc
241 tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc
301 ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga
361 gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc
421 caacgtggac cagtcggccc tctcttgctc cctgtctgcc gatggcatgc tgaccttctg
481 tggccccaag atccagactg gcctggatgc cacccacgcc gagcgagcca tccccgtgtc
541 gcgggaggag aagcccacct cggctccctc gtcctaagca ggcattgcct cggctg
601
//
LOCUS BM705919 598 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-d-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-d-20-0-UI 5', mRNA sequence.
ACCESSION BM705919
VERSION BM705919.1 GI:19019177
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 598)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..598
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-d-20-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 90 a 208 c 181 g 117 t 2 others
ORIGIN
1 tggcctggat gccacccacg ccgagcgagc catccccgtg tcgcgggagg agaagcccac
61 ctcggctccc tcgtcctaag caggctttgc ctcggctggc tcccctgcag ccctggccca
121 tcatgggggg agcaccctga gggcggggtg tctgtcttcc tttgcttccc ttttttcctt
181 tccaccttct cacatggaat gagggtttga gagagcagcc aggagagctt agggtctcag
241 ggtgtcccag accccgacac cggccagtgg cggaagtgac cgcacctcac actcctttag
301 atagcagcct ggctcccctg gggtgcaggc gcctcaactc tgctgagggt ccagaaggag
361 ggggtgacct ccggccaggt gcctcctgac acacctgcag cctccctccg cggcgggccc
421 tgcccacacc tcctggggcg cgtgaggccc gtggggccgg ggcttctgtg cacctgggct
481 ctcgcggnct cttctctcag accgtcttcc tccancccct ctatgtagtg ccgctcttgg
541 ggacatgggt cgcccatgag agcgcagccc gcggcaatca ataaacagca ggtgatac
601
//
LOCUS BM697434 585 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-p-20-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-p-20-0-UI 5', mRNA sequence.
ACCESSION BM697434
VERSION BM697434.1 GI:19010692
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 585)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..585
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-p-20-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 102 a 199 c 170 g 114 t
ORIGIN
1 tgaagcactt ctccccggag gacctcaccg tgaaggtgca ggacgacttt gtggagatcc
61 acggaaagca caacgagcgc caggacgacc acggctacat ttcccgtgag ttccaccgcc
121 gctaccgcct gccgtccaac gtggaccagt cggccctctc ttgctccctg tctgccgatg
181 gcatgctgac cttctgtggc cccaagatcc agactggcct ggatgccacc cacgccgagc
241 gagccatccc cgtgtcgcgg gaggagaagc ccacctcggc tccctcgtcc taagcaggca
301 ttgcctcggc tggctcccct gcagccctgg cccatcatgg ggggagcacc ctgagggcgg
361 ggtgtctgtc ttcctttgct tccctttttt cctttccacc ttctcacatg gaatgagggt
421 ttgagagagc agccaggaga gcttagggtc tcagggtgtc ccagaccccg acaccggcca
481 gtggcggaag tgaccgcacc tcacactcct ttagatagca gcctggctcc cctggggtgc
541 aggcgcctca actctgctga gggtccagaa ggagggggtg acctc
//
LOCUS BM721893 578 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahw-c-15-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahw-c-15-0-UI 5', mRNA sequence.
ACCESSION BM721893
VERSION BM721893.1 GI:19042101
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 578)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..578
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahw-c-15-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 99 a 216 c 148 g 115 t
ORIGIN
1 gaggccccgc tgactcctgc cagcctccag gtccccgtgg taccaaagct gaacatggac
61 gtgaccatcc agcacccctg gttcaagcgc accctggggc ccttctaccc cagccggctg
121 ttcgaccagt ttttcggcga gggccttttt gagtatgacc tgctgccctt cctgtcgtcc
181 accatcagcc cctactaccg ccagtccctc ttccgcaccg tgctggactc cggcatctct
241 gaggttcgat ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg
301 gaggacctca ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag
361 cgccaggacg accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc
421 aacgtggacc agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt
481 ggccccaaga tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg
541 cgggaggaga agcccacctc ggctccctcg tcctaagc
//
LOCUS BM706029 585 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agg-m-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agg-m-06-0-UI 5', mRNA sequence.
ACCESSION BM706029
VERSION BM706029.1 GI:19019287
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 585)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..585
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agg-m-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 84 a 206 c 177 g 117 t 1 others
ORIGIN
1 gccgatggca tgctgacctt ctgtggcccc aagatccaga ctggcctgga tgccacccac
61 gccgagcgag ccatccccgt gtcgcgggag gagaagccca cctcggctcc ctcgtcctaa
121 gcaggcattg cctcggctgg ctcccctgca gccctggccc atcatggggg gagcaccctg
181 agggcggggt gtctgtcttc ctttgcttcc cttttttcct ttccaccttc tcacatggaa
241 tgagggtttg agagagcagc caggagagct tagggtctca gggtgtccca gaccccgaca
301 ccggccagtg gcggaagtga ccgcacctca cactccttta gatagcagcc tggctcccct
361 ggggtgcagg cgcctcaact ctgctgaggg tccagaagga gggggtgacc tccggccagg
421 tgcctcctga cacacctgca gcctccctcc gcggcgggcc ctgcccacac ctcctggggc
481 gcgtgaggcc cgtggggccg gggcttctgt gcacctgggc tctcgcggnc tcttctctca
541 gaccgtcttc ctccaacccc tctatgtagt ggcgctcttg gggac
//
LOCUS BM696799 588 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-p-02-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-p-02-0-UI 5', mRNA sequence.
ACCESSION BM696799
VERSION BM696799.1 GI:19010057
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 588)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..588
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-p-02-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 101 a 218 c 150 g 118 t 1 others
ORIGIN
1 aggccccgct gactcctgcc agcctccagg tccccgtggt accaaagctg aacatggacg
61 tgaccatcca gcacccctgg ttcaagcgca ccctggggcc cttctacccc agccggctgt
121 tcgaccagtt tttcggcgag ggcctttttg agtatgacct gctgcccttc ctgtcgtcca
181 ccatcagccc ctactaccgc cagtccctct tccgcaccgt gctggactcc ggcatctctg
241 aggttcgatc cgaccgggac aagttcgtca tcttcctcga tgtgaagcac ttctccccgg
301 aggacctcac cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc
361 gccaggacga ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca
421 acgtggacca gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg
481 gccccaagat ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc
541 gggaggagaa gccacctcgg ntccctcgtc ctaagcaggc attgcctc
//
LOCUS BM722650 586 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahy-a-10-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-a-10-0-UI 5', mRNA sequence.
ACCESSION BM722650
VERSION BM722650.1 GI:19043377
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 586)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..586
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-a-10-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 100 a 219 c 148 g 119 t
ORIGIN
1 cccgctgact cctgccagcc tccaggtccc cgtggtacca aagctgaaca tggatgtgac
61 catccagcac ccctggttca agcgcaccct ggggcccttc taccccagcc ggctgttcga
121 ccagtttttc ggcgagggcc tttttgagta tgacctgctg cccttcctgt cgtccaccat
181 cagcccctac taccgccagt ccctcttccg caccgtgctg gactccggca tctctgaggt
241 tcgatccgac cgggacaagt tcgtcatctt cctcgatgtg aagcacttct ccccggagga
301 cctcaccgtg aaggtgcagg acgactttgt ggagatccac ggaaagcaca acgagcgcca
361 ggacgaccac ggctacattt cccgtgagtt ccaccgccgc taccgcctgc cgtccaacgt
421 ggaccagtcg gccctctctt gctccctgtc tgccgatggc atgctgacct tctgtggccc
481 caagatccag actggcctgg atgccacccc acgccgagcg agccatcccc gtgtcgcggg
541 aggagaagcc cacctcggct ccctcgtcct aagcaggcat tgcctc
//
LOCUS BM697368 569 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-d-06-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-d-06-0-UI 5', mRNA sequence.
ACCESSION BM697368
VERSION BM697368.1 GI:19010626
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 569)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..569
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-d-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 95 a 211 c 148 g 114 t 1 others
ORIGIN
1 gaccatccag cacccctggt tcaagcgcac cctggggccc ttctacccca gccggctgtt
61 cgaccagttt ttcggcgagg gcctttttga gtatgacctg ctgcccttcc tgtcgtccac
121 catcagcccc tactaccgcc agtccctctt ccgcaccgtg ctggactccg gcatctctga
181 ggttcgatcc gaccgggaca agttcgtcat cttcctcgat gtgaagcact tctccccgga
241 ggacctcacc gtgaaggtgc aggacgactt tgtggagatc cacggaaagc acaacgagcg
301 ccaggacgac cacggctaca tttcccgtga gttccaccgc cgctaccgcc tgccgtccaa
361 cgtggaccag tcggccctct cttgctccct gtctgccgat ggnatgctga ccttctgtgg
421 ccccaagatc cagactggcc tggatgccac ccacgccgag cgagccatcc ccgtgtcgcg
481 ggaggagaag cccacctcgg ctccctcgtc ctaagcaggc attgcctcgg ctgactcccc
541 tgcagccctg gcccatcatg gggggagca
//
LOCUS BM664316 583 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-DW0-agh-b-24-0-UI.s1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-b-24-0-UI 3', mRNA sequence.
ACCESSION BM664316
VERSION BM664316.1 GI:18970340
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 583)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..583
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-b-24-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI).
TAG_LIB=UI-E-DW0 TAG_TISSUE=human lens
TAG_SEQ=CGATTAGCGA"
BASE COUNT 111 a 172 c 193 g 106 t 1 others
ORIGIN
1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcanaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg
541 cttaggacga gggagccgag gtgggcttct cctcccgcga cac
//
LOCUS BM722196 564 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahy-o-03-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-o-03-0-UI 5', mRNA sequence.
ACCESSION BM722196
VERSION BM722196.1 GI:19042608
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 564)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..564
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-o-03-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 99 a 194 c 156 g 113 t 2 others
ORIGIN
1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca
61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg
121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc
181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga
241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga
301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc
361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt
421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg
481 gtctcagggt gtcncagacc ccgacaccgg ncagtggcgg aagtgaccgc acctcacact
541 cctttagata gcagcctggc tccc
//
LOCUS BM706139 580 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agg-b-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agg-b-16-0-UI 5', mRNA sequence.
ACCESSION BM706139
VERSION BM706139.1 GI:19019397
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 580)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..580
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agg-b-16-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 101 a 213 c 149 g 116 t 1 others
ORIGIN
1 agaggccccg ctgactcctg ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga
61 tgtgaccatc cagcacccct ggttcaagcg caccctgggg cccttctacc ccagccggct
121 gttcgaccag tttttcggcg agggcctttt tgagtatgac ctgctgccct tcctgtcgtc
181 caccatcagc ccctactacc gccagtccct cttccgcacc gtgctggact ccggcatctc
241 tgaggttcga tccgaccggg acaagttcgt catcttcctc gatgtgaagc acttctcccc
301 ggaggacctc accgtgaagg tgcaggacga ctttgtggag atccacggaa agcacaacga
361 gcgccaggac gaccacggct acatttcccg tgagttccac cgccgctacc gcctgccgtc
421 caacgtggac cagtcggccc tctcttgctc nctgtctgcc gatggcatgc tgaccttctg
481 tggccccaag atccagactg ggctggatgc cacccacgcc gagcgagcca tccccgtgtc
541 gcgggaggag aagcccaact cggctcccct cgtcctaagc
//
LOCUS BM705929 560 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-f-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-f-24-0-UI 5', mRNA sequence.
ACCESSION BM705929
VERSION BM705929.1 GI:19019187
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 560)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..560
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-f-24-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 99 a 192 c 156 g 112 t 1 others
ORIGIN
1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca
61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg
121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc
181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga
241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga
301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc
361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt
421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg
481 gtctcagggt gtcncagacc ccgacaccgg ccagtggcgg aagtgaccgc acctcacact
541 cctttagata gcagcctggc
//
LOCUS BM722779 559 bp mRNA linear EST 01-MAR-2002
DEFINITION UI-E-EO0-ahy-l-01-0-UI.r1 UI-E-EO0 Homo sapiens cDNA clone
UI-E-EO0-ahy-l-01-0-UI 5', mRNA sequence.
ACCESSION BM722779
VERSION BM722779.1 GI:19043602
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 559)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..559
/db_xref="taxon:9606"
/tissue_type="fetal eye"
/clone="UI-E-EO0-ahy-l-01-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-EO0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EO0 is a cDNA library containing the following
tissue(s): fetal eye. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is CGCGTATACC. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 97 a 206 c 142 g 113 t 1 others
ORIGIN
1 ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga tgtgaccatc cagcacccct
61 ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag tttttcggcg
121 agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc ccctactacc
181 gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga tccgaccggg
241 acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc accgtgaagg
301 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct
361 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc
421 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg
481 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct
541 cggntccctc gtcctaagc
//
LOCUS BM696667 558 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agk-o-10-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-o-10-0-UI 5', mRNA sequence.
ACCESSION BM696667
VERSION BM696667.1 GI:19009925
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 558)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..558
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-o-10-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 97 a 206 c 143 g 112 t
ORIGIN
1 ccagcctcca ggtccccgtg gtaccaaagc tgaacatgga cgtgaccatc cagcacccct
61 ggttcaagcg caccctgggg cccttctacc ccagccggct gttcgaccag tttttcggcg
121 agggcctttt tgagtatgac ctgctgccct tcctgtcgtc caccatcagc ccctactacc
181 gccagtccct cttccgcacc gtgctggact ccggcatctc tgaggttcga tccgaccggg
241 acaagttcgt catcttcctc gatgtgaagc acttctcccc ggaggacctc accgtgaagg
301 tgcaggacga ctttgtggag atccacggaa agcacaacga gcgccaggac gaccacggct
361 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcgggcc
421 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg
481 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagccacctc
541 ggctccctcg tcctaagc
//
LOCUS BM705911 549 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agh-b-24-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agh-b-24-0-UI 5', mRNA sequence.
ACCESSION BM705911
VERSION BM705911.1 GI:19019169
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 549)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..549
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agh-b-24-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 97 a 188 c 152 g 111 t 1 others
ORIGIN
1 ccgaccggga caagttcgtc atcttcctcg atgtgaagca cttctccccg gaggacctca
61 ccgtgaaggt gcaggacgac tttgtggaga tccacggaaa gcacaacgag cgccaggacg
121 accacggcta catttcccgt gagttccacc gccgctaccg cctgccgtcc aacgtggacc
181 agtcggccct ctcttgctcc ctgtctgccg atggcatgct gaccttctgt ggccccaaga
241 tccagactgg cctggatgcc acccacgccg agcgagccat ccccgtgtcg cgggaggaga
301 agcccacctc ggctccctcg tcctaagcag gcattgcctc ggctggctcc cctgcagccc
361 tggcccatca tggggggagc accctgaggg cggggtgtct gtcttccttt gcttcccttt
421 tttcctttcc accttctcac atggaatgag ggtttgagag agcagccagg agagcttagg
481 gtctcagggt gtcncagacc ccgacaccgg ccagtggcgg aagtgaccgc acctcacact
541 cctttagat
//
LOCUS BM667923 574 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-DW0-agk-o-15-0-UI.s1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agk-o-15-0-UI 3', mRNA sequence.
ACCESSION BM667923
VERSION BM667923.1 GI:18975754
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 574)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..574
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agk-o-15-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI).
TAG_LIB=UI-E-DW0 TAG_TISSUE=human lens
TAG_SEQ=CGATTAGCGA"
BASE COUNT 110 a 166 c 190 g 106 t 2 others
ORIGIN
1 tttttttttt ttttttttgc ntgtatcacc tgctgtttat tgattgccgc gggctgtgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct ccccccatga tgggccaggg ctgcagggga gccagccgag gcaatgcctg
541 cttangacga gggagccgag gtgggcttct cctc
//
LOCUS BM698588 556 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DX0-agr-e-16-0-UI.r1 UI-E-DX0 Homo sapiens cDNA clone
UI-E-DX0-agr-e-16-0-UI 5', mRNA sequence.
ACCESSION BM698588
VERSION BM698588.1 GI:19011846
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 556)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..556
/db_xref="taxon:9606"
/tissue_type="fetal eyes"
/clone="UI-E-DX0-agr-e-16-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="fetal"
/organism="Homo sapiens"
/clone_lib="UI-E-DX0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DX0 is a cDNA library containing the following
tissue(s): fetal eyes. The library was constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis
of first-strand cDNA contains a library tag sequence that
is located between the Not I site and the (dT)18 tail.
The sequence tag for this library is AGAATCAAGA. This
library was created for the program, Gene Discovery in
the Visual System, supported by National Eye Institute
(NEI)."
BASE COUNT 94 a 193 c 160 g 108 t 1 others
ORIGIN
1 ccacggaaag cacaacgagc gccaggacga ccacggctac atttcccgtg agttccaccg
61 ccgctaccgc ctgccgtcca acgtggacca gtcggccctc tcttgctccc tgtctgccga
121 tggcatgctg accttctgtg gcctcaagat ccagactggc ctggatgcca cccacgccga
181 gcgagccatc cccgtgtcgc gggaggagaa gcccacctcg gctccctcgt cctaagcagg
241 cattgcctcg gctggctccc ctgcagccct ggcccatcat ggggggagca ccctgagggc
301 ggggtgtctg tcttcctttg cttccctttt ttcctttcca ccttctcaca tggaatgagg
361 gtttgagaga gcagccagga gagcttaggg tcttagggtg tcncagaccc cgacaccggc
421 cagtggcgga agtgaccgca cctcacactc ctttagatag cagcctggct cccctggggt
481 gcaggcgcct caactctgct gagggtccag aaggaggggg tgacctccgg ccaggtgcct
541 cctgacacac ctgcag
//
LOCUS BM697422 547 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-n-16-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-n-16-0-UI 5', mRNA sequence.
ACCESSION BM697422
VERSION BM697422.1 GI:19010680
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 547)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..547
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-n-16-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 84 a 198 c 156 g 108 t 1 others
ORIGIN
1 acatttcccg tgagttccac cgccgctacc gcctgccgtc caacgtggac cagtcggccc
61 tctcttgctc cctgtctgcc gatggcatgc tgaccttctg tggccccaag atccagactg
121 gcctggatgc cacccacgcc gagcgagcca tccccgtgtc gcgggaggag aagcccacct
181 cggctccctc gtcctaagca ggcattgcct cggctggctc ccctgcagcc ctggcccatc
241 atggggggag caccctgagg gcggggtgtc tgtcttcctt tgcttccctt ttttcctttc
301 caccttctca catggaatga gggtttgaga gagcagccag gagagcttag ggtctcaggg
361 tgtcccagac cccgacaccg gccagtggcg gaagtgaccg cacctcacac tcctttagat
421 agcagcctgg ctcccctggg gtgcaggcgc ctcaactctg ctgagggtcc agaaggaggg
481 ggtgacctcc ggccaggtgc ctcctgacac acctgcagcc tccctccgcg gngggccctg
541 cccacac
//
LOCUS BM668935 571 bp mRNA linear EST 27-FEB-2002
DEFINITION UI-E-DW0-agg-i-06-0-UI.s2 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agg-i-06-0-UI 3', mRNA sequence.
ACCESSION BM668935
VERSION BM668935.1 GI:18978832
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 571)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Forward POLYA=Yes.
FEATURES Location/Qualifiers
source 1..571
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agg-i-06-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI).
TAG_LIB=UI-E-DW0 TAG_TISSUE=human lens
TAG_SEQ=CGATTAGCGA"
BASE COUNT 111 a 165 c 189 g 105 t 1 others
ORIGIN
1 tttttttttt ttttttttgc ttgtatcacc tgctgtttat tgattgccgc gggctgcgct
61 ctcatgggcg acccatgtcc ccaagagcgg cactacatag aggggttgga ggaagacggt
121 ctgagagaag aggccgcgag agcccaggtg cacagaagcc ccggccccac gggcctcacg
181 cgccccagga ggtgtgggca gggcccgccg cggagggagg ctgcaggtgt gtcaggaggc
241 acctggccgg aggtcacccc ctccttctgg accctcagca gagttgaggc gcctgcaccc
301 caggggagcc aggctgctat ctaaaggagt gtgaggtgcg gtcacttccg ccactggccg
361 gtgtcggggt ctgggacacc ctgagaccct aagctctcct ggctgctctc tcaaaccctc
421 attccatgtg agaaggtgga aaggaaaaaa gggaagcaaa ggaagacaga caccccgccc
481 tcagggtgct cccccatgat gggccaaggc tgcaggggag ccanccgagg caatgcctgc
541 ttaggacgag ggagccgagg tgggcttctc c
//
LOCUS BM697425 536 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agm-n-22-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agm-n-22-0-UI 5', mRNA sequence.
ACCESSION BM697425
VERSION BM697425.1 GI:19010683
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 536)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..536
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agm-n-22-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 91 a 183 c 155 g 107 t
ORIGIN
1 cgtgaaggtg caggacgact ttgtggagat ccacggaaag cacaacgagc gccaggacga
61 ccacggctac atttcccgtg agttccaccg ccgctaccgc ctgccgtcca acgtggacca
121 gtcggccctc tcttgctccc tgtctgccga tggcatgctg accttctgtg gccccaagat
181 ccagactggc ctggatgcca cccacgccga gcgagccatc cccgtgtcgc gggaggagaa
241 gcccacctcg gctccctcgt cctaagcagg cattgcctcg gctggctccc ctgcagccct
301 ggcccatcat ggggggagca ccctgagggc ggggtgtctg tcttcctttg cttccctttt
361 ttcctttcca ccttctcaca tggaatgagg gtttgagaga gcagccagga gagcttaggg
421 tctcagggtg tcccagaccc cgacaccggc cagtggcgga agtgaccgca cctcacactc
481 ctttagatag cagcctggct cccctggggt gcaggcgcct caactctgct gagggt
541
//
LOCUS BM705774 534 bp mRNA linear EST 28-FEB-2002
DEFINITION UI-E-DW0-agi-h-12-0-UI.r1 UI-E-DW0 Homo sapiens cDNA clone
UI-E-DW0-agi-h-12-0-UI 5', mRNA sequence.
ACCESSION BM705774
VERSION BM705774.1 GI:19019032
KEYWORDS EST.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 534)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
MEDLINE 97044477
COMMENT Contact: Soares, MB Program for Rat Gene Discovery and Mapping
University of Iowa 451 Eckstein Medical Research Building Iowa
City, IA 52242, USA Tel: 319 335 8250 Fax: 319 335 9565 Email:
msoares@blue.weeg.uiowa.edu Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa Clone
Distribution: Researchers may obtain clones from Research Genetics
(www.resgen.com). Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..534
/db_xref="taxon:9606"
/tissue_type="lens"
/clone="UI-E-DW0-agi-h-12-0-UI"
/lab_host="DH10B (Life Technologies) (T1 phage
resistant)"
/dev_stage="adult"
/organism="Homo sapiens"
/clone_lib="UI-E-DW0"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-DW0 is a cDNA library containing the following
tissue(s): lens. The library was constructed according to
Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an
oligo-dT primer containing a Not I site. Double stranded
cDNA was ligated to an EcoR I adaptor, digested with Not
I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CGATTAGCGA. This library
was created for the program, Gene Discovery in the Visual
System, supported by National Eye Institute (NEI)."
BASE COUNT 85 a 191 c 144 g 113 t 1 others
ORIGIN
1 agtttttcgg cgagggcctt tttgagtatg acctgctgcc cttcctgtcg tccaccatca
61 gcccctacta ccgccagtcc ctcttccgca ccgtgctgga ctccggcatc tctgaggttc
121 gatccgaccg ggacaagttc gtcatcttcc tcgatgtgaa gcacttctcc ccggaggacc
181 tcaccgtgaa ggtgcaggac gactttgtgg agatccacgg aaagcacaac gagcgccagg
241 acgaccacgg ctacatttc